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Literature summary for 3.2.1.81 extracted from

  • Lakshmikanth, M.; Manohar, S.; Souche, Y.; Lalitha, J.
    Extracellular beta-agarase LSL-1 producing neoagarobiose from a newly isolated agar-liquefying soil bacterium, Acinetobacter sp., AG LSL-1 (2006), World J. Microbiol. Biotechnol., 22, 1087-1094.
No PubMed abstract available
Search for more literature for 3.2.1.81

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Acinetobacter sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 PAGE Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
agarose + H2O Acinetobacter sp. an endo type glycosidase, which specifically cleaves beta-1,4 linkages found in agarose neoagarohexaose + neoagarotetraose + neoagarobiose neoagarobiose is the final hydrolysis product ?
agarose + H2O Acinetobacter sp. AG LSL-1 an endo type glycosidase, which specifically cleaves beta-1,4 linkages found in agarose neoagarohexaose + neoagarotetraose + neoagarobiose neoagarobiose is the final hydrolysis product ?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
-
-
Acinetobacter sp.
-
 enzyme is inducible by agar and not repressed by other simple sugars. NaCl is not required for growth or production of agarase. Enzyme belongs to group III beta-agarase family
-
Acinetobacter sp. AG LSL-1
-
-
-
Acinetobacter sp. AG LSL-1
-
 enzyme is inducible by agar and not repressed by other simple sugars. NaCl is not required for growth or production of agarase. Enzyme belongs to group III beta-agarase family
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O
-
 Acinetobacter sp. neoagarobiose
-
 ?
agarose + H2O
-
 Acinetobacter sp. AG LSL-1 neoagarobiose
-
 ?
agarose + H2O an endo type glycosidase, which specifically cleaves beta-1,4 linkages found in agarose Acinetobacter sp. neoagarohexaose + neoagarotetraose + neoagarobiose neoagarobiose is the final hydrolysis product ?
agarose + H2O an endo type glycosidase, which specifically cleaves beta-1,4 linkages found in agarose Acinetobacter sp. AG LSL-1 neoagarohexaose + neoagarotetraose + neoagarobiose neoagarobiose is the final hydrolysis product ?
additional information no substrate: kappa-carrageenan, alginate, cellulose, carboxymethyl cellulose Acinetobacter sp. ?
-
 ?
additional information alpha-1,3-glycosidase activity is not detected Acinetobacter sp. ?
-
 ?
additional information no substrate: kappa-carrageenan, alginate, cellulose, carboxymethyl cellulose Acinetobacter sp. AG LSL-1 ?
-
 ?
additional information alpha-1,3-glycosidase activity is not detected Acinetobacter sp. AG LSL-1 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE Acinetobacter sp.

Synonyms

Synonyms Comment Organism
agarase
-
 Acinetobacter sp.
LSL-1
-
 Acinetobacter sp.

Expression

Organism Comment Expression
Acinetobacter sp. the enzyme is inducible by agar up