Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 54% residual activity | Bacillus subtilis | |
EDTA | 1 mM, 37% residual activity | Bacillus subtilis | |
Mg2+ | 1 mM, 53% residual activity | Bacillus subtilis | |
Mn2+ | 1 mM, 22% residual activity | Bacillus subtilis | |
N-bromosuccinimide | 1 mM, 3% residual activity | Bacillus subtilis | |
Ni2+ | 1 mM, 61% residual activity | Bacillus subtilis | |
sodium dodecylsulfate | 1 mM, 55% residual activity | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | apparent Km values of the mannanase for locust bean gum, guar gum and konjac powder are 7.6, 10.5 and 27.4 mg/ml, respectively | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 126% of initial activity | Bacillus subtilis | |
Fe2+ | 1 mM, 124% of initial activity | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39600 | - |
x * 39600, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
induced by growth on konjac powder as carbon source at 50°C | - |
Bacillus subtilis WY34 | - |
induced by growth on konjac powder as carbon source at 50°C | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate content of 13.1% | Bacillus subtilis |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8302 | - |
pH 6.0, 50°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | poor substrate | Bacillus subtilis | ? | - |
? | |
carboxymethyl cellulose + H2O | poor substrate | Bacillus subtilis WY34 | ? | - |
? | |
locust bean gum + H2O | - |
Bacillus subtilis | ? | - |
? | |
locust bean gum + H2O | - |
Bacillus subtilis WY34 | ? | - |
? | |
mannan + H2O | source of substrate: copra | Bacillus subtilis | mannotetraose + mannobiose + mannotriose | - |
? | |
mannan + H2O | source of substrate: copra | Bacillus subtilis WY34 | mannotetraose + mannobiose + mannotriose | - |
? | |
starch + H2O | poor substrate | Bacillus subtilis | ? | - |
? | |
starch + H2O | poor substrate | Bacillus subtilis WY34 | ? | - |
? | |
xylan + H2O | poor substrate | Bacillus subtilis | ? | - |
? | |
xylan + H2O | poor substrate | Bacillus subtilis WY34 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39600, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
at pH 6.0, stable up to | Bacillus subtilis |
75 | - |
30 min, 90% loss of activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.1 | - |
Bacillus subtilis |