Cloned (Comment) | Organism |
---|---|
CDase I-5 gene, pBR322, expression in Escherichia coli MC1061 | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.83 | - |
beta-cyclodextrin | - |
Bacillus sp. (in: Bacteria) | |
0.92 | - |
gamma-cyclodextrin | - |
Bacillus sp. (in: Bacteria) | |
1.23 | - |
alpha-cyclodextrin | - |
Bacillus sp. (in: Bacteria) | |
2.11 | - |
pullulan | - |
Bacillus sp. (in: Bacteria) | |
3.01 | - |
soluble starch | - |
Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
MW deduced from the nucleotide sequence 64884 | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
I-5 | - |
Bacillus sp. (in: Bacteria) I-5 | - |
I-5 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acarbose + H2O | pseudotetrasaccharide inhibitor of glucosidases and alpha-amylases, binding the active sites of the enzyme | Bacillus sp. (in: Bacteria) | ? | - |
? | |
acarbose + H2O | pseudotetrasaccharide inhibitor of glucosidases and alpha-amylases, binding the active sites of the enzyme | Bacillus sp. (in: Bacteria) I-5 | ? | - |
? | |
alpha-cyclomaltodextrin + H2O | - |
Bacillus sp. (in: Bacteria) | alpha-D-glucose + maltose | - |
? | |
alpha-cyclomaltodextrin + H2O | - |
Bacillus sp. (in: Bacteria) I-5 | alpha-D-glucose + maltose | - |
? | |
beta-cyclodextrin + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
beta-cyclodextrin + H2O | - |
Bacillus sp. (in: Bacteria) I-5 | ? | - |
? | |
soluble starch + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
soluble starch + H2O | - |
Bacillus sp. (in: Bacteria) I-5 | ? | - |
? |