Information on EC 3.2.1.54 - cyclomaltodextrinase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.2.1.54
-
RECOMMENDED NAME
GeneOntology No.
cyclomaltodextrinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cyclomaltodextrin + H2O = linear maltodextrin
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
hydrolysis of O-glycosyl bond
additional information
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
-
-
starch degradation III
-
-
starch degradation IV
-
-
starch degradation
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SYSTEMATIC NAME
IUBMB Comments
cyclomaltodextrin dextrin-hydrolase (decyclizing)
Also hydrolyses linear maltodextrin.
CAS REGISTRY NUMBER
COMMENTARY hide
37288-41-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 7324
-
-
Manually annotated by BRENDA team
strain 7324
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
7364
TREMBL
Manually annotated by BRENDA team
7364
TREMBL
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
H-17, transformed with Bacillus caldolyticus C2 DNA
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
ssp. lactis
-
-
Manually annotated by BRENDA team
ssp. lactis
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain A11
-
-
Manually annotated by BRENDA team
strain A11
-
-
Manually annotated by BRENDA team
strain R-47
-
-
Manually annotated by BRENDA team
strain R-47
-
-
Manually annotated by BRENDA team
strain 39E
-
-
Manually annotated by BRENDA team
strain B1001
-
-
Manually annotated by BRENDA team
isolated from a Paralvinella sp. polychaete worm collected from an active deep-sea hydrothermal vent sulfide chimney, gene CL1_0884 or tccd
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Env Cda13A can be classified into the neopullulanase subfamily consisting of neopullulanases, cyclomaltodextrinases and maltogenic amylases
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-
Manually annotated by BRENDA team
strain K-11151, contains a bifunctional enzyme with combined activities of alpha-amylase and CD-/pullulan-degrading enzyme
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Manually annotated by BRENDA team
strain K-11151, contains a bifunctional enzyme with combined activities of alpha-amylase and CD-/pullulan-degrading enzyme
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-
Manually annotated by BRENDA team
additional information
DNA isolated from Bos taurus rumen fluid
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
the characteristic of the enzyme hydrolyzing beta-cyclodextrin at a relatively low temperature and nonneutral pH should play an important role in the survival of Thermococcus kodakarensis KOD1 under low temperature conditions (65°C)
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-O-alpha-maltosylglucose + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-alpha-D-maltohexaoside + H2O
4-nitrophenol + maltohexaose
show the reaction diagram
4-nitrophenyl-alpha-D-maltopentaoside + H2O
4-nitrophenol + alpha-D-maltopentaoside
show the reaction diagram
-
-
-
-
?
4-O-alpha-D-glucosylsucrose + H2O
?
show the reaction diagram
-
-
-
-
?
6-O-alpha-D-glucosyl-alpha-cyclodextrin + H2O
isomaltooligosaccharides
show the reaction diagram
branched cyclodextrin
-
-
?
6-O-alpha-D-glucosyl-beta-cyclodextrin + H2O
isomaltooligosaccharides
show the reaction diagram
branched cyclodextrin
-
-
?
6-O-alpha-D-maltosyl-beta-cyclodextrin + H2O
isomaltooligosaccharides
show the reaction diagram
branched cyclodextrin
-
-
?
acarbose + H2O
?
show the reaction diagram
acarbose + H2O
acarviosine-glucose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
acarbose + H2O
D-glucose + maltose
show the reaction diagram
-
-
-
?
alpha-cyclodextrin
maltotriose + maltose + glucose
show the reaction diagram
alpha-cyclodextrin + H2O
?
show the reaction diagram
alpha-cyclodextrin + H2O
D-glucose + maltose
show the reaction diagram
alpha-cyclodextrin + H2O
glucose + maltose + maltotriose
show the reaction diagram
alpha-cyclodextrin + H2O
isomaltooligosaccharides
show the reaction diagram
alpha-cyclodextrin + H2O
linear maltodextrins
show the reaction diagram
alpha-cyclodextrin + H2O
maltooligosaccharides
show the reaction diagram
alpha-cyclodextrin + H2O
maltose + D-glucose
show the reaction diagram
alpha-cyclodextrin + H2O
maltose + glucose
show the reaction diagram
alpha-cyclomaltodextrin + H2O
alpha-D-glucose + maltose
show the reaction diagram
amylopectin + H2O
?
show the reaction diagram
-
-
-
-
?
amylopectin + H2O
maltose + ?
show the reaction diagram
amylose + H2O
?
show the reaction diagram
amylose + H2O
alpha-D-glucose + maltose
show the reaction diagram
amylose + H2O
maltose + glucose
show the reaction diagram
-
CDase I-5 shows high preference towards amylose
major hydrolysis products
-
?
beta-cyclodextrin + H2O
?
show the reaction diagram
beta-cyclodextrin + H2O
D-glucose
show the reaction diagram
beta-cyclodextrin is the most active substrate
-
-
?
beta-cyclodextrin + H2O
D-glucose + maltose
show the reaction diagram
beta-cyclodextrin + H2O
glucose + maltose + long chain linear oligomers
show the reaction diagram
beta-cyclodextrin + H2O
glucose + maltose + maltotriose + maltotetraose
show the reaction diagram
beta-cyclodextrin + H2O
maltooligosaccharides
show the reaction diagram
beta-cyclodextrin + H2O
maltose + D-glucose
show the reaction diagram
beta-cyclodextrin + H2O
maltose + glucose
show the reaction diagram
beta-cyclodextrin + H2O
maltose + maltotriose + maltotetraose + maltopentaose + maltoheptaose + glucose
show the reaction diagram
beta-cyclodextrin + H2O
panose + maltoheptaose
show the reaction diagram
-
cyclomaltodextrinase activity
-
-
?
beta-cyclomaltodextrin + H2O
alpha-D-glucose + maltose
show the reaction diagram
branched cyclodextrins + H2O
glucose + maltose
show the reaction diagram
-
-
-
?
cyclodextrin + H2O
glucose
show the reaction diagram
cyclodextrin + H2O
panose + maltoheptaose
show the reaction diagram
cyclomaltodextrin + H2O
alpha-D-glucose + maltose
show the reaction diagram
-
composed of 6 to 8 D-glucopyranosyl residues
-
-
?
cyclomaltodextrin + H2O
linear maltodextrin
show the reaction diagram
cyclomaltodextrin + H2O
maltodextrin
show the reaction diagram
cyclomaltodextrin + H2O
maltooligosaccharide
show the reaction diagram
cyclomaltodextrins + H2O
linear maltodextrins
show the reaction diagram
cyclomaltoheptaose + H2O
?
show the reaction diagram
cyclomaltohexaose + H2O
?
show the reaction diagram
-
-
-
-
?
dextrin + H2O
?
show the reaction diagram
G1-alpha-cyclodextrin + H2O
D-glucose + maltose
show the reaction diagram
-
24% of the activity with beta-cyclodextrin
main products
-
?
G1-beta-cyclodextrin + H2O
D-glucose + maltose
show the reaction diagram
-
32% of the activity with beta-cyclodextrin
main products
-
?
G2-alpha-cyclodextrin + H2O
D-glucose + maltose
show the reaction diagram
-
19% of the activity with beta-cyclodextrin
main products
-
?
G2-beta-cyclodextrin + H2O
D-glucose + maltose
show the reaction diagram
-
30% of the activity with beta-cyclodextrin
main products
-
?
gamma-cyclodextrin + H2O
?
show the reaction diagram
gamma-cyclodextrin + H2O
D-glucose
show the reaction diagram
hydrolyzing activity is about 20% compared to the activity with beta-cyclodextrin
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-
?
gamma-cyclodextrin + H2O
D-glucose + maltose
show the reaction diagram
gamma-cyclodextrin + H2O
glucose + maltose + maltotriose + maltotetraose + maltopentaose + maltohexaose + maltoheptaose + maltooctaose
show the reaction diagram
gamma-cyclodextrin + H2O
maltooctaose + ?
show the reaction diagram
gamma-cyclodextrin + H2O
maltooligosaccharides
show the reaction diagram
gamma-cyclodextrin + H2O
maltose + D-glucose
show the reaction diagram
gamma-cyclodextrin + H2O
maltose + glucose
show the reaction diagram
gamma-cyclomaltodextrin + H2O
alpha-D-glucose + maltose
show the reaction diagram
glucosyl-alpha-cyclodextrin + H2O
6,3-O-alpha-D-glucosylmaltohexaose
show the reaction diagram
glucosyl-beta-cyclodextrin + H2O
6,4-O-alpha-D-glucosylmaltoheptaose
show the reaction diagram
glycogen + H2O
?
show the reaction diagram
-
relative rate of hydrolysis 0,1%
-
-
?
hydroxyethyl-beta-cyclodextrin + H2O
?
show the reaction diagram
-
-
-
-
?
hydroxypropyl-beta-cyclodextrin + H2O
?
show the reaction diagram
-
-
-
-
?
isopanose + H2O
?
show the reaction diagram
linear maltodextrins + H2O
alpha-D-glucose + maltose
show the reaction diagram
linear maltoheptaose + H2O
alpha-D-glucose + maltose
show the reaction diagram
-
-
-
-
?
linear maltohexaose + H2O
alpha-D-glucose + maltose
show the reaction diagram
-
-
-
-
?
linear maltooctaose + H2O
alpha-D-glucose + maltose
show the reaction diagram
-
-
-
-
?
maltodextrin + H2O
maltose
show the reaction diagram
-
-
-
-
?
maltodextrins + H2O
maltose + glucose
show the reaction diagram
maltoheptaose + H2O
?
show the reaction diagram
maltoheptaose + H2O
D-glucose + maltose
show the reaction diagram
maltoheptaose + H2O
maltose + D-glucose
show the reaction diagram
maltohexaose + H2O
?
show the reaction diagram
maltohexaose + H2O
D-glucose + maltose
show the reaction diagram
maltohexaose + H2O
maltose + D-glucose
show the reaction diagram
-
14% of the activity with gamma-cyclodextrin
-
-
?
maltohexaose + H2O
maltotetraose + maltose
show the reaction diagram
maltooctaose + H2O
maltohexaose + maltose
show the reaction diagram
maltooligosaccharides + H2O
D-glucose
show the reaction diagram
maltooligosaccharides + H2O
maltotriose + maltotetraose
show the reaction diagram
-
alpha-amylase activity
main products
-
?
maltopentaose + H2O
?
show the reaction diagram
maltopentaose + H2O
D-glucose + maltose
show the reaction diagram
maltopentaose + H2O
maltose + D-glucose
show the reaction diagram
-
11% of the activity with gamma-cyclodextrin
-
-
?
maltopentaose + H2O
maltose + maltotriose
show the reaction diagram
maltosyl-alpha-cyclodextrin + H2O
6,3-O-alpha-D-maltosylmaltohexaose
show the reaction diagram
maltosyl-beta-cyclodextrin
6,4-O-alpha-D-maltosylmaltoheptaose
show the reaction diagram
maltotetraose + H2O
2 maltose
show the reaction diagram
maltotetraose + H2O
?
show the reaction diagram
maltotetraose + H2O
D-glucose + maltose
show the reaction diagram
maltotetraose + H2O
maltose + D-glucose
show the reaction diagram
maltotriitol + H2O
?
show the reaction diagram
-
-
-
-
?
maltotriose + H2O
?
show the reaction diagram
maltotriose + H2O
D-glucose + maltose
show the reaction diagram
maltotriose + H2O
maltose + D-glucose
show the reaction diagram
methyl-beta-cyclodextrin + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-D-maltopentaoside + H2O
p-nitrophenol + maltopentaose
show the reaction diagram
-
-
-
-
?
phenyl alpha-maltoside + H2O
?
show the reaction diagram
-
-
-
-
?
pullulan + H2O
?
show the reaction diagram
pullulan + H2O
branched pullulan tetrasaccharide
show the reaction diagram
-
substrate is a linear polysaccharide composed of maltotriose units linked by alpha-1,6-bonds, low activity
main products
-
?
pullulan + H2O
D-glucose
show the reaction diagram
hydrolyzing activity is about 20% compared to the activity with beta-cyclodextrin
-
-
?
pullulan + H2O
glucose + panose + isopanose + large saccharides containing alpha 1,6-glucosidic bonds
show the reaction diagram
pullulan + H2O
maltotriose
show the reaction diagram
pullulan + H2O
panose
show the reaction diagram
pullulan + H2O
panose + beta-cyclodextrin
show the reaction diagram
-
-
-
-
?
pullulan + H2O
panose + maltoheptaose
show the reaction diagram
-
cyclomaltodextrinase activity
-
-
?
pullulan + H2O
panose + maltose
show the reaction diagram
pullulan + H2O
panose + maltose + glucose
show the reaction diagram
soluble starch + H2O
?
show the reaction diagram
starch + H2O
alpha-D-glucose + maltose
show the reaction diagram
starch + H2O
D-glucose
show the reaction diagram
starch + H2O
glucose + maltose
show the reaction diagram
starch + H2O
maltooligosaccharides
show the reaction diagram
starch + H2O
maltotriose + maltotetraose
show the reaction diagram
-
alpha-amylase activity, preferred substrate
main products
-
?
amylopectin + H2O
D-glucose
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-cyclodextrin + H2O
isomaltooligosaccharides
show the reaction diagram
cyclomaltodextrins + H2O
linear maltodextrins
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Al3+
-
activates 46.8% at 5 mM
Ba2+
-
relative activity 101.5%
Cu2+
-
inhibitory at 1 mM
K+
activates lsightly at 5 mM
Na+
activates lsightly at 5 mM
Ni2+
-
inhibitory at 1 mM
Zn2+
-
inhibitory at 1 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
-
5 mM, 37% inhibition
1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide
-
5 mM, complete inhibition
acarbose
AgNO3
-
10 mM, complete inhibition
Al3+
82% inhibition at 5 mM
BaCl2
-
10 mM, 12% inhibition
Ca2+
inhibits slightly at 5 mM
Cd2+
97% inhibition at 5 mM
Co2+
29% inhibition at 5 mM
Cr3+
-
relative activity 58.4%
CuSO4
-
10 mM, complete inhibition
diethyldicarbonate
Diethylpyrocarbonate
-
1 mM, complete inhibition
DMSO
61%inhibition at 10%
dodecylbenzenesulfonate
-
0.1%
EDTA
-
at 20 mM
ethanol
complete inhibition at 10%
FeSO4
-
10 mM, 68% inhibition
HgCl2
-
10 mM, complete inhibition
iodoacetamide
Li+
-
relative activity 75.5%
methanol
99% inhibition at 10%
MgCl2
MnCl2
-
10 mM, 33% inhibition
N-bromosuccinimide
p-chloromercuribenzoate
Phenylglyoxal
ZnCl2
EDTA
additional information
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-deoxy-D-glucose
-
0.01 M, relative activity 140%
dithiothreitol
-
recombinant enzyme
DMSO
-
11% activation at 10% w/v
EGTA
-
increases activity about 2.3fold, relative activity 236.3%
ethanol
-
13% activation at 10% w/v
glucosamine
-
0.01 M, relative activity 133%
glucose
-
0.01 M, relative activity 143%
glucose 6-phosphate
-
0.005 M, relative activity 111%
maltose
-
0.01 M, relative activity 140%
miglitol
-
1 mg/ml, relative activity 107%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
21
2-O-alpha-maltosylglucose
-
-
17
4-O-alpha-D-glucosylsucrose
-
-
11.1
acarbose
-
pH 4.5, 90°C; pH 4.5, 90°C, recombinant enzyme