Application | Comment | Organism |
---|---|---|
analysis | comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major | Phlebotomus papatasi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
mannose | relative inhibition of 67.8%, potent inhibitor, three concentrations of 10, 50, and 100 mM tested | Phlebotomus papatasi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phlebotomus papatasi | - |
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid) | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
whole body | whole fly homogenates | Phlebotomus papatasi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown, enzyme activity of female colonies of the Jordan Valley autumn (arid) differs significantly from those of colonies of Neot Hakikar (oasis) and Kfar Adumim starved (arid) | Phlebotomus papatasi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-D-mannopyranoside + H2O | activity measured in whole fly homogenates, substrate concentration of 60 mM in citrate buffer, female oasis flies have 90% less alpha-mannosidase than females from the arid desert, flies of arid regions tend to produce more alpha-mannosidase activity than those originating in sugar-rich environments | Phlebotomus papatasi | 4-nitrophenol + alpha-D-mannopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-mannosidase | - |
Phlebotomus papatasi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | - |
assay at, pH-optimum estimated for | Phlebotomus papatasi |