Information on EC 3.2.1.24 - alpha-mannosidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
3.2.1.24
-
RECOMMENDED NAME
GeneOntology No.
alpha-mannosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
transglycosylation
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
d-mannose degradation
-
-
Other glycan degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-D-mannoside mannohydrolase
Also hydrolyses alpha-D-lyxosides and heptopyranosides with the same configuration at C-2, C-3 and C-4 as mannose.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-42-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
Pekin-type drake
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
-
-
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Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
alpha-mannosidase from Erythrina indica has N-terminal amino acid sequence as DTQEN
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
monkey
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-
Manually annotated by BRENDA team
alfalfa
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-
Manually annotated by BRENDA team
Neosartorya fischeri
-
-
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Manually annotated by BRENDA team
rabbit
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid)
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-
Manually annotated by BRENDA team
extremophilic organism belonging to the euryarchaeal phylum
UniProt
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
black cherry
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
sea squirt
-
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Manually annotated by BRENDA team
expressed in Pichia pastoris
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-alpha-D-mannobiose + H2O
alpha-D-mannose
show the reaction diagram
-
-
-
?
1,3-alpha-D-mannobiose + H2O
alpha-D-mannose
show the reaction diagram
-
-
-
?
1,6-alpha-D-mannobiose + H2O
alpha-D-mannose
show the reaction diagram
-
-
-
?
1-naphthyl-alpha-D-mannopyranoside + H2O
1-naphthol + alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
2 Man9GlcNAc + 3 H2O
Man8GlcNAc + Man7GlcNAc + 3 alpha-D-mannopyranose
show the reaction diagram
-
-
products from unbound fraction of cobalt-chelating Sepharose
?
2,4-dinitrophenyl alpha-D-mannopyranoside + H2O
2,4-dinitrophenol + alpha-D-mannopyranose
show the reaction diagram
2,4-dinitrophenyl alpha-D-mannoside + H2O
2,4-dinitrophenol + alpha-D-mannose
show the reaction diagram
-
-
-
-
?
2,4-dinitrophenyl-alpha-D-mannopyranoside + H2O
2,4-dinitrophenol + alpha-D-mannopyranose
show the reaction diagram
-
-
-
-
?
2,5-dinitrophenyl-alpha-D-mannopyranoside + H2O
2,4-dinitrophenol + D-mannose
show the reaction diagram
-
-
-
-
?
2-acetamido-4-O-alpha-D-mannopyranosyl-2-deoxy-D-glucose + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
2-deoxy-2-fluoro-alpha-D-mannosyl fluoride + H2O
?
show the reaction diagram
-
substrate for wild-type enzyme and very slow substrate of mutant enzyme D341N
-
-
?
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
alpha-D-mannopyranose
show the reaction diagram
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
alpha-D-mannopyranose + D-mannopyranose
show the reaction diagram
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
D-mannopyranose
show the reaction diagram
2-O-alpha-D-mannotriose + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
highly specific for alpha-1,2-mannosidic linkages
-
?
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O
alpha-D-mannopyranose
show the reaction diagram
the enzyme is only active with the alpha-1,3-linked disaccharide
-
-
?
3-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
2 alpha-D-mannopyranose
show the reaction diagram
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
4-methylumbelliferol + alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranose
show the reaction diagram
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannose
show the reaction diagram
Neosartorya fischeri
-
pH 6.5, 50C, 15 min, reaction stopped with Na2CO3
-
-
?
4-methylumbelliferyl-alpha-D-mannopyranoside
?
show the reaction diagram
-
-
-
?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranose
show the reaction diagram
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + D-mannose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl alpha-D-mannopyranoside + H2O
4-nitrophenol + alpha-D-mannopyranose
show the reaction diagram
4-nitrophenyl alpha-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
show the reaction diagram
4-nitrophenyl alpha-D-mannoside + H2O
4-nitrophenol + alpha-D-mannose
show the reaction diagram
4-nitrophenyl-alpha-D-mannopyranoside + H2O
4-nitrophenol + alpha-D-mannopyranose
show the reaction diagram
4-nitrophenyl-alpha-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
show the reaction diagram
Neosartorya fischeri
-
-
-
-
?
4-nitrophenyl-alpha-D-mannopyranoside + H2O
4-nitrophenyl + alpha-D-mannose
show the reaction diagram
Neosartorya fischeri
-
-
-
-
?
4-nitrophenyl-alpha-D-mannoside + H2O
4-nitrophenol + alpha-D-mannose
show the reaction diagram
4-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
2 alpha-D-mannopyranose
show the reaction diagram
-
specific cleavage of alpha-1,4- or alpha-1,6-linkages
-
?
5-fluoro-beta-L-gulosyl fluoride + H2O
?
show the reaction diagram
-
acts as a slow substrate for the D341 mutant enzyme, with deglycosylation as the rate-limiting step. Inactivation is only observed when assayed at low temperatures such that deglycosylation is slow relative to the assay time
-
-
?
6-bromo-2-naphthyl-alpha-D-mannopyranoside + H2O
6-bromo-2-naphthol + alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
6-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
alpha-D-mannopyranose
show the reaction diagram
alpha-(1-2)-mannobiose + H2O
alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
alpha-(1-2)-mannobiose + H2O
alpha-D-mannopyranose + D-mannopyranose
show the reaction diagram
-
-
-
?
alpha-(1-3)-mannobiose + H2O
alpha-D-mannopyranose
show the reaction diagram
-
highest activity and affinity
-
?
alpha-(1-3)-mannobiose + H2O
alpha-D-mannopyranose + D-mannopyranose
show the reaction diagram
-
highest activity and affinity
-
?
alpha-(1-6)-mannobiose
alpha-D-mannopyranose + D-mannopyranose
show the reaction diagram
-
low activity
-
?
alpha-(1-6)-mannobiose + H2O
alpha-D-mannopyranose
show the reaction diagram
-
low activity
-
?
alpha-conglutin + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
lupin seed storage glycoprotein, not active to beta- or gamma-conglutin
-
?
alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + 6 H2O
6 alpha-D-mannopyranose + alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc
show the reaction diagram
alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + H2O
alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc + alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)-beta-D-Man
show the reaction diagram
-
cleaves asparagine-linked high mannose oligosaccharides to form the core Man3GlcNAc2
-
?
alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc + H2O
alpha-D-mannose + ?
show the reaction diagram
alpha-D-Man-(1->2)-D-Man + H2O
2 alpha-D-mannose
show the reaction diagram
alpha-D-Man-(1->3)-D-Man + H2O
2 alpha-D-mannose
show the reaction diagram
alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-D-GlcNAc + H2O
alpha-D-mannose + ?
show the reaction diagram
-
-
-
?
alpha-D-Man-(1->4)-D-Man + H2O
2 alpha-D-mannose
show the reaction diagram
alpha-D-Man-(1->6)-D-Man + H2O
2 alpha-D-mannose
show the reaction diagram
alpha-D-mannopyranoside + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
?
alpha-D-mannosidosis fibroblasts + H2O
Man7-2GlcNAc + ?
show the reaction diagram
-
-
-
?
alpha-D-mannosidosis fibroblasts + H2O
Manbeta(1-4)GlcNAc + ?
show the reaction diagram
-
-
?
alpha3,alpha6-D-mannopentaose + H2O
alpha-D-mannose
show the reaction diagram
-
-
-
?
beta-glucuronidase peptide + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
GDP-mannosylpyranose + H2O
alpha-D-mannopyranose + GDP
show the reaction diagram
GDP-mannosylpyranose + H2O
alpha-D-mannosylpyranose + GDP
show the reaction diagram
Glc3Man9GlcNAc2 + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
from oligosaccharide-lipid
-
?
GlcNAcMan5GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
glycopeptide + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
glycoprotein + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
high mannose N-glycan + H2O
?
show the reaction diagram
-
-
-
?
Man-alpha(1-3)Man + H2O
alpha-D-mannose
show the reaction diagram
Neosartorya fischeri
-
pH 6.5, 50C, 20 min
-
-
?
Man-alpha(1-6)Man + H2O
alpha-D-mannose
show the reaction diagram
Neosartorya fischeri
-
pH 6.5, 50C, 20 min
-
-
?
Man-alpha-(1-2)-Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]-Manalpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]-Man-beta(1-4)GlcNAc + H2O
?
show the reaction diagram
-
-
-
-
?
Man-alpha-(1-2)-Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]-Manalpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]-Man-beta(1-4)GlcNAc + H2O
Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha(1-3)]-Man-beta-(1-4)-GlcNAc + alpha-mannose
show the reaction diagram
-
-
end product in presence of Co2+. Before treatment with Co(II) the enzyme is able to cleave a single Manalpha1-2 residue from the substrate to give Man-alpha(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]Man-beta-(1-4)-GlcNAc as the end product
-
?
Man-glycopeptide + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
low activity
-
?
Man3GlcNAc2 + H2O
Man2GlcNAc2 + alpha-D-mannopyranose
show the reaction diagram
-
Man-1,6-linkage only hydrolysed after its Man-1,3-residue is removed
-
?
Man5-9GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
low activity for Man5GlcNAc
-
?
Man5GlcNAc + 2 H2O
Man3GlcNAc + 2 alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
Man5GlcNAc2 + H2O
Man-GlcNAc2 + mannose
show the reaction diagram
-
-
-
-
?
Man5GlcNAc2 + H2O
Man3GlcNAc2 + alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
Man5GlcNAc2 + H2O
Man3GlcNAc2 + mannose
show the reaction diagram
-
-
-
-
?
Man5GlcNAc2 + H2O
Man4GlcNAc2 + alpha-D-mannopyranose
show the reaction diagram
-
cleaves Man-alpha-(1-3)-linkage 10times faster than the Man-1,6 and the Man-1,2-linkages
mixture of three isomeric oligosaccharides
?
Man6 + H2O
alpha-D-mannose + ?
show the reaction diagram
-
derived from ovalbumin, alpha-1,2: 1,3: 1,6-linkages, more than 10fold higher specific activity for isoform EII than for EI
-
?
Man6-glycopeptide + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Man6GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
Man6GlcNAc2 + H2O
Man-GlcNAc2 + mannose
show the reaction diagram
-
-
-
-
?
Man6GlcNAc2 + H2O
Man5GlcNAc2 + mannose
show the reaction diagram
-
slow reaction of the Golgi alpha-mannosidase in vitro
-
-
?
Man7GlcNAc + 2 H2O
Man5GlcNAc + 2 alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
Man7GlcNAc2 + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
from oligosaccharide-lipid, very low activity
-
?
Man7GlcNAc2 + H2O
Man-GlcNAc2 + mannose
show the reaction diagram
-
-
-
-
?
Man8GlcNAc + 3 H2O
Man5GlcNAc + 3 alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
Man8GlcNAc2 + 3 H2O
Man5GlcNAc2 + 3 alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
Man8GlcNAc2 + 3 H2O
Man5GlcNAc2 + 3 D-mannose
show the reaction diagram
Man8GlcNAc2 + H2O
Man-GlcNAc2 + mannose
show the reaction diagram
-
-
-
-
?
Man9-5GlcNAc + 4 H2O
Man5-3GlcNAc + 4 alpha-D-mannopyranose
show the reaction diagram
-
different product composition for alpha-mannosidases IA, IB and II
-
?
Man9-glycopeptide + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Man9GlcNAc + 4 H2O
Man5GlcNAc + 4 alpha-D-mannopyranose
show the reaction diagram
Man9GlcNAc + H2O
Man8GlcNAc + alpha-D-mannopyranose
show the reaction diagram
Man9GlcNAc + H2O
Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc + alpha-D-mannopyranose
show the reaction diagram
-
Co2+ activated alpha-mannosidase
end products
?
Man9GlcNAc2 + H2O
Man-GlcNAc2 + mannose
show the reaction diagram
-
-
-
-
?
Man9GlcNAc2 + H2O
Man5-8GlcNAc2 + alpha-D-mannopyranose
show the reaction diagram
Man9GlcNAc2 + H2O
Man6GlcNAc2 + mannose
show the reaction diagram
-
fast reaction of the Golgi alpha-mannosidase in vitro
-
-
?
Man9GlcNAc2 + H2O
Man8GlcNAc2 + mannose
show the reaction diagram
Manalpha(1-2)Man + H2O
D-mannose
show the reaction diagram
Neosartorya fischeri
-
-
-
-
-
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNac-betaGlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manbeta(1-6)]ManbetaGlcNAc-beta-GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-6)Manalpha(1-4)-anhydromannitol + H2O
Manalpha(1-2)Manalpha(1-6)Manalpha(1-4)-anhydromannitol + Manalpha(1-6)Manalpha(1-4)-anhydromannitol + Manalpha(1-4)anhydromannitol + anhydromannitol + alpha-D-mannose
show the reaction diagram
-
-
equal amounts, but only traces of Manalpha(1-4)-anhydromannitol
?
Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Manalpha(1-3)GlcNAc + H2O
alpha-D-mannopyranose + 2-acetylamino-2-deoxy-D-glucose
show the reaction diagram
-
-
-
?
Manalpha(1-3)GlcNAc + H2O
alpha-D-mannopyranose + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
Manalpha(1-3)Man + H2O
D-mannose
show the reaction diagram
Neosartorya fischeri
-
-
-
-
-
Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Manalpha(1-4)Manbeta(1-4)Man + H2O
alpha-D-mannopyranose + Manbeta(1-4)Man
show the reaction diagram
-
-
-
?
Manalpha(1-6)GlcNAc + H2O
alpha-mannopyranose + 2-acetylamino-2-deoxy-D-glucose
show the reaction diagram
-
-
-
?
Manalpha(1-6)GlcNAc + H2O
alpha-mannopyranose + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
Manalpha(1-6)Man + H2O
D-mannose
show the reaction diagram
Neosartorya fischeri
-
-
-
-
-
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-)]Manbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
4 times faster hydrolysis than Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Manalpha1-2Man + H2O
alpha-mannose
show the reaction diagram
-
-
-
-
?
Manalpha1-3Man + H2O
alpha-mannose
show the reaction diagram
-
-
-
-
?
Manalpha1-6Man + H2O
alpha-mannose
show the reaction diagram
-
-
-
-
?
mannan + H2O
alpha-D-mannose + ?
show the reaction diagram
mannopyranosyl-oligosaccharides + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
mannosyl rhamnose + H2O
alpha-D-mannopyranose + rhamnose
show the reaction diagram
-
part of the repeating oligosaccharide unit of the O-antigenic lipopolysaccharide in Salmonella typhimurium
-
?
mannosyl(5)-N-acetyl-glucosamine-pyridylamino + H2O
mannosyl(4)-N-acetyl-glucosamine-pyridylamino + mannosyl(3)-N-acetyl-glucosamine-pyridylamino
show the reaction diagram
-
occurrence of mannosyl(4)-N-acetyl-glucosamine-pyridylamino (32%) and mannosyl(3)-N-acetyl-glucosamine-pyridylamino (8.1%) are observed only in the presence of Co2+
-
?
mannosyl(9)-N-acetyl-glucosamine + H2O
mannosyl(5)-N-acetyl-glucosamine + alpha-D-mannose
show the reaction diagram
in presence of Co2+
-
-
?
mannosyl(9)-N-acetyl-glucosamine + H2O
mannosyl(8)-N-acetyl-glucosamine + alpha-D-mannose
show the reaction diagram
absence of Co2+
-
-
?
mannotetraose + H2O
alpha-D-mannopyranose + mannotriose
show the reaction diagram
mannotriose + H2O
alpha-D-mannopyranose + mannobiose
show the reaction diagram
-
-
-
?
methyl-2,4,6-tri-O-methyl-D-mannopyranoside + H2O
2,4,6-tri-O-methyl-D-mannopyranose + methanol
show the reaction diagram
-
-
-
?
methyl-2-O-alpha-D-mannopyranosyl-D-mannopyranoside + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
very low activity
-
?
methyl-4-O-alpha-D-mannopyranosyl-D-mannopyranoside + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
methyl-alpha-D-mannnopyranose + H2O
alpha-D-mannopyranose + methanol
show the reaction diagram
-
-
-
?
methyl-alpha-D-mannopyranoside + H2O
alpha-D-mannopyranose + methanol
show the reaction diagram
methyl-O-1,4-alpha-D-mannobiose + H2O
alpha-D-mannose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-rhamnopyranoside + ethyl 1-thio-alpha-D-rhamnopyranoside
p-nitrophenol + ethyl alpha-D-rhamnopyranosyl-(1-2)-1-thio-alpha-D-rhamnopyranoside
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-mannoside + H2O
p-nitrophenol + alpha-mannose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
4-nitrophenol + alpha-D-mannose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
p-nitrophenol + alpha-D-mannopyranose
show the reaction diagram
p-nitrophenyl-alpha-D-mannopyranoside + H2O
p-nitrophenol + alpha-D-mannose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-mannoside + H2O
p-nitrophenol + alpha-D-mannose
show the reaction diagram
-
-
-
-
?
phenyl-alpha-D-mannopyranoside + H2O
alpha-D-mannopyranose + phenol
show the reaction diagram
-
-
-
?
pyridylamino-glycopeptide + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
from ovalbumin
-
?
pyridylamino-Man5GlcNAc + H2O
pyridylamino-Man2GlcNA + alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
pyridylamino-Man5GlcNAc + H2O
pyridylamino-Man4GlcNAc + pyridylamino-Man3-2GlcNAc + alpha-D-mannose
show the reaction diagram
-
lesser degree of pyridylamino-Man3-2GlcNAc, stepwise cleavage to Man2GlcNAc structures
?
pyridylamino-Man9GlcNAc2 + H2O
pyridylamino-Man3-8GlcNAc + Man2GlcNAc + alpha-D-mannopyranose
show the reaction diagram
pyridylamino-Man9GlcNAc2 + H2O
pyridylamino-Man8GlcNAc2 + alpha-D-mannopyranose
show the reaction diagram
-
high amounts of substrate hydrolysed at neutral pH, very little cleavage at pH 3.0
hydrolysis products within 20 min
?
ribonuclease B + H2O
oligosaccharide + alpha-D-mannopyranose + ?
show the reaction diagram
RNase-B-Man(n)-glycoform + H2O
RNase-B-Man(n-1)-glycoform + alpha-D-mannose
show the reaction diagram
-
-
-
?
TAKA-amylase A + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
thyroglobulin + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
thyroglobulin peptide A + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 Man9GlcNAc + 3 H2O
Man8GlcNAc + Man7GlcNAc + 3 alpha-D-mannopyranose
show the reaction diagram
-
-
products from unbound fraction of cobalt-chelating Sepharose
?
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
alpha-D-mannopyranose
show the reaction diagram
2-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
D-mannopyranose
show the reaction diagram
2-O-alpha-D-mannotriose + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
highly specific for alpha-1,2-mannosidic linkages
-
?
3-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
2 alpha-D-mannopyranose
show the reaction diagram
4-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
2 alpha-D-mannopyranose
show the reaction diagram
-
specific cleavage of alpha-1,4- or alpha-1,6-linkages
-
?
6-O-alpha-D-mannopyranosyl-D-mannopyranose + H2O
alpha-D-mannopyranose
show the reaction diagram
alpha-(1-2)-mannobiose + H2O
alpha-D-mannopyranose + D-mannopyranose
show the reaction diagram
-
-
-
?
alpha-(1-3)-mannobiose + H2O
alpha-D-mannopyranose + D-mannopyranose
show the reaction diagram
-
highest activity and affinity
-
?
alpha-(1-6)-mannobiose
alpha-D-mannopyranose + D-mannopyranose
show the reaction diagram
-
low activity
-
?
alpha-conglutin + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
lupin seed storage glycoprotein, not active to beta- or gamma-conglutin
-
?
alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + 6 H2O
6 alpha-D-mannopyranose + alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc
show the reaction diagram
alpha-D-Man-(1-2)-alpha-D-Man-(1-2)-alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)-[alpha-D-Man-(1-2)-alpha-D-Man-(1-3)]-alpha-D-Man-(1-6)]-beta-D-Man-(1-4)-beta-D-GlcNAc + H2O
alpha-D-Man-(1-6)-[alpha-D-Man-(1-3)]-beta-D-Man-(1-4)-beta-D-GlcNAc + alpha-D-Man-(1-3)-[alpha-D-Man-(1-6)]-alpha-D-Man-(1-6)-beta-D-Man
show the reaction diagram
-
cleaves asparagine-linked high mannose oligosaccharides to form the core Man3GlcNAc2
-
?
alpha-D-mannopyranoside + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
O09159
-
-
?
alpha-D-mannosidosis fibroblasts + H2O
Man7-2GlcNAc + ?
show the reaction diagram
-
-
-
?
alpha-D-mannosidosis fibroblasts + H2O
Manbeta(1-4)GlcNAc + ?
show the reaction diagram
O09159
-
-
?
beta-glucuronidase peptide + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
GDP-mannosylpyranose + H2O
alpha-D-mannopyranose + GDP
show the reaction diagram
Glc3Man9GlcNAc2 + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
from oligosaccharide-lipid
-
?
GlcNAcMan5GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
glycopeptide + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
glycoprotein + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
high mannose N-glycan + H2O
?
show the reaction diagram
Q99YP5
-
-
-
?
Man-alpha-(1-2)-Man-alpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-3)]-Manalpha-(1-6)-[Man-alpha-(1-2)-Man-alpha-(1-2)-Man-alpha-(1-3)]-Man-beta(1-4)GlcNAc + H2O
?
show the reaction diagram
-
-
-
-
?
Man-glycopeptide + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
low activity
-
?
Man3GlcNAc2 + H2O
Man2GlcNAc2 + alpha-D-mannopyranose
show the reaction diagram
-
Man-1,6-linkage only hydrolysed after its Man-1,3-residue is removed
-
?
Man5-9GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
low activity for Man5GlcNAc
-
?
Man5GlcNAc + 2 H2O
Man3GlcNAc + 2 alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
Man5GlcNAc2 + H2O
Man3GlcNAc2 + alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
Man5GlcNAc2 + H2O
Man4GlcNAc2 + alpha-D-mannopyranose
show the reaction diagram
-
cleaves Man-alpha-(1-3)-linkage 10times faster than the Man-1,6 and the Man-1,2-linkages
mixture of three isomeric oligosaccharides
?
Man6 + H2O
alpha-D-mannose + ?
show the reaction diagram
-
derived from ovalbumin, alpha-1,2: 1,3: 1,6-linkages, more than 10fold higher specific activity for isoform EII than for EI
-
?
Man6-glycopeptide + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Man6GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
Man7GlcNAc + 2 H2O
Man5GlcNAc + 2 alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
Man7GlcNAc2 + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
from oligosaccharide-lipid, very low activity
-
?
Man8GlcNAc + 3 H2O
Man5GlcNAc + 3 alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
Man8GlcNAc2 + 3 H2O
Man5GlcNAc2 + 3 alpha-D-mannopyranose
show the reaction diagram
-
-
-
?
Man8GlcNAc2 + 3 H2O
Man5GlcNAc2 + 3 D-mannose
show the reaction diagram
-
Golgi alpha-mannosidase in vivo
-
-
?
Man9-5GlcNAc + 4 H2O
Man5-3GlcNAc + 4 alpha-D-mannopyranose
show the reaction diagram
-
different product composition for alpha-mannosidases IA, IB and II
-
?
Man9-glycopeptide + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Man9GlcNAc + 4 H2O
Man5GlcNAc + 4 alpha-D-mannopyranose
show the reaction diagram
Man9GlcNAc + H2O
Man8GlcNAc + alpha-D-mannopyranose
show the reaction diagram
-
highly specific soluble catalytic domain of a larger membrane-bound form, does not act on synthetic substrates
releases only one specific mannopyranose residue
?
Man9GlcNAc + H2O
Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAc + alpha-D-mannopyranose
show the reaction diagram
-
Co2+ activated alpha-mannosidase
end products
?
Man9GlcNAc2 + H2O
Man5-8GlcNAc2 + alpha-D-mannopyranose
show the reaction diagram
Man9GlcNAc2 + H2O
Man8GlcNAc2 + mannose
show the reaction diagram
-
endoplasmic reticulum alpha-mannosidase in vivo
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNac-betaGlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manbeta(1-6)]ManbetaGlcNAc-beta-GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Manalpha(1-2)Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Manalpha(1-3)GlcNAc + H2O
alpha-D-mannopyranose + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
Manalpha(1-3)[Man(1-6)]ManbetaGlcNAc-betaGlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
Manalpha(1-4)Manbeta(1-4)Man + H2O
alpha-D-mannopyranose + Manbeta(1-4)Man
show the reaction diagram
-
-
-
?
Manalpha(1-6)GlcNAc + H2O
alpha-mannopyranose + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-)]Manbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
4 times faster hydrolysis than Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
?
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
mannan + H2O
alpha-D-mannose + ?
show the reaction diagram
mannopyranosyl-oligosaccharides + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
mannosyl rhamnose + H2O
alpha-D-mannopyranose + rhamnose
show the reaction diagram
-
part of the repeating oligosaccharide unit of the O-antigenic lipopolysaccharide in Salmonella typhimurium
-
?
mannotetraose + H2O
alpha-D-mannopyranose + mannotriose
show the reaction diagram
mannotriose + H2O
alpha-D-mannopyranose + mannobiose
show the reaction diagram
-
-
-
?
ribonuclease B + H2O
oligosaccharide + alpha-D-mannopyranose + ?
show the reaction diagram
TAKA-amylase A + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
thyroglobulin + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
-
-
-
?
thyroglobulin peptide A + H2O
alpha-D-mannopyranose + ?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cr2+
-
weak activation
Fe2+
4.5fold activation at a concentration of 10 mM
Ni2+
-
0.1 mM, enhances activity to 119% of control
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,6R,7R,8S)-7,8-dihydroxy-5-thia-1-thioniabicyclo[4.3.0]nonane chloride
-
synthetic inhibitor, selective and potent inhibition at 1 mM, 97% inhibition of the activity of the liver lysosomal fraction at pH 4.0, 100% at pH 6.5
(1S,2R,3R,8R,8aR)-3-[(arylmethoxy)methyl]octahydroindolizine-1,2,8-triol
-
-
(1S,2R,8R,8aR)-octahydroindolizine-1,2,8-triol
(2R,3R,4S)-2-(aminomethyl)pyrrolidine-3,4-diol
(2R,3R,4S)-2-([(1R)-2,3-dihydro-1H-inden-1-ylamino]methyl)pyrrolidine-3,4-diol
-
IC50 is 0.017 mM
(2R,3R,4S)-2-([[(1R)-1-hydroxyethyl]amino]methyl)pyrrolidine-3,4-diol
(2R,3R,4S)-2-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidine-3,4-diol
(2R,3R,4S)-2-([[(1S)-1-hydroxyethyl]amino]methyl)pyrrolidine-3,4-diol
(2R,3R,4S)-2-hydroxymethyl-pyrrolidine-3,4-diol
(2R,3R,4S)-2-phenylaminomethyl-pyrrolidine-3,4-diol
(2R,3R,4S)-2-[(1-phenyl-ethylamino)-methyl]-pyrrolidine-3,4-diol
(2R,3R,4S)-2-[(benzylamino)methyl]pyrrolidine-3,4-diol
-
IC50 is 0.06 mM
(2R,3R,4S)-2-[(cyclopentylamino)methyl]pyrrolidine-3,4-diol
(2R,3R,4S)-2-[([(1R)-2-benzyloxy-1-phenylethyl]amino)methyl]pyrrolidine-3,4-diol
-
92% inhibition at 1 mM, IC50 is 0.058 mM
(2R,3R,4S)-2-[([(1R)-2-hydroxy-1-phenylethyl]amino)methyl]pyrrolidine-3,4-diol
(2R,3R,4S)-2-[([(1R)-2-methoxy-1-phenylethyl]amino)methyl]pyrrolidine-3,4-diol
-
77% inhibition at 1 mM
(2R,3R,4S)-2-[([(1R,2S)-2-hydroxy-1,2-diphenylethyl]amino)methyl]pyrrolidine-3,4-diol
-
88% inhibition at 1 mM, IC50 is 0.11 mM
(2R,3R,4S)-2-[([(1S)-2-hydroxy-1-phenylethyl]amino)methyl]pyrrolidine-3,4-diol
(2R,3R,4S)-2-[([(1S,2R)-2-hydroxy-1,2-diphenylethyl]amino)methyl]pyrrolidine-3,4-diol
-
84% inhibition at 1 mM, IC50 is 0.128 mM
(2R,3R,4S)-2-[2-(phenylamino)ethyl]pyrrolidine-3,4-diol
-
1 mM, 33% inhibition
(2R,3R,4S)-2-[[(1-benzyl-piperidin-4-ylmethyl)-amino]-methyl]-pyrrolidine-3,4-diol
(2R,3R,4S)-2-[[(2H-Imidazol-1-ylmethyl)-amino]-methyl]-pyrrolidine-3,4-diol
(2R,3R,4S)-2-[[(furan-2-ylmethyl)-amino]-methyl]-pyrrolidine-3,4-diol
(2R,3R,4S)-2-[[(thiophen-2-ylmethyl)-amino]-methyl]-pyrrolidine-3,4-diol
(2R,3R,4S,5R)-2-[(benzylamino)methyl]-5-(hydroxymethyl)pyrrolidine-3,4-diol
-
IC50 is 0.0062 mM
(2R,3S,4R,5R)-2-(hydroxymethyl)-5-[([(1R)-2-hydroxy-1,2-diphenylethyl]amino)methyl]pyrrolidine-3,4-diol
-
98% inhibition at 1 mM, IC50 is 0.0042 mM
(2S)-2-[([(2R,3S,4S)-3,4-dihydroxypyrrolidin-2-yl]methyl)amino]-2-phenylethyl 2-fluorobenzoate
(2S)-2-[([(2R,3S,4S)-3,4-dihydroxypyrrolidin-2-yl]methyl)amino]-2-phenylethyl 3-bromobenzoate
(2S)-2-[([(2R,3S,4S)-3,4-dihydroxypyrrolidin-2-yl]methyl)amino]-2-phenylethyl 4-bromobenzoate
(2S)-2-[([(2R,3S,4S)-3,4-dihydroxypyrrolidin-2-yl]methyl)amino]-2-phenylethyl 4-fluorobenzoate
(3R,4R,5R)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)-1-methylpyrrolidin-2-one
(3R,4R,5R)-3,4-dihydroxy-5-([[(1R)-2-hydroxy-1-phenylethyl]amino]methyl)pyrrolidin-2-one
(3S,4R)-pyrrolidine-3,4-diol
(6-butoxy-3-oxo-3,6-dihydro-2H-pyran-2-yl)methyl 2,2-dimethylpropanoate
-
-
(6-methoxy-3-oxo-3,6-dihydro-2H-pyran-2-yl)methyl 2,2-dimethylpropanoate
-
-
1,6-dideoxy-1,6-episulfinyl-beta-D-mannose
-
XLM, sulfoxide derivative, thiolevomannosan analog, more potent than kifunensine and deoxymannojirimycin, docking studies with the crystal structure of human alpha-1,2-mannosidase complexed with kifunensine (PDB: 1FO3) as a template
1,6-dideoxy-1,6-episulfonyl-beta-D-mannose
-
NLM, sulfone derivative, thiolevomannosan analog, more potent than kifunensine and deoxymannojirimycin, docking studies with the crystal structure of human alpha-1,2-mannosidase complexed with kifunensine (PDB: 1FO3) as a template
1,6-dideoxy-1,6-epithio-beta-D-mannose
-
thiolevomannosan TLM, docking studies with the crystal structure of human alpha-1,2-mannosidase complexed with kifunensine (PDB: 1FO3) as a template
1-cyclohexyl-3-(2-morpholinyl-4-ethyl)carbodiimide
-
inhibitory at 25 mM, pseudo-first-order kinetic
1-deoxy-mannojirimycin
-
complete inhibition
1-deoxymannojirimicin
1-deoxymannojirimycin
2-(2,2-dihydroxy-ethyl)-pyrrolidine-3,4-diol
2-amino-2-deoxy-D-glucose
-
competitive inhibition, Ki = 2.8 mM
2-deoxy-2-fluoro-alpha-D-mannosyl fluoride
-
for mutant enzyme D341N, no inhibition of wild-type enzyme
3-bromo-N-[(2S)-2-[([(2R,3S,4R)-3,4-dihydroxypyrrolidin-2-yl]methyl)amino]-2-phenylethyl]benzamide
5-fluoro-beta-L-gulosyl fluoride
-
reversible, acts as a slow substrate for the D341 mutant enzyme, with deglycosylation as the rate-limiting step. Inactivation is only observed when assayed at low temperatures such that deglycosylation is slow relative to the assay time
8,8a-di-epi-swainsonine
-
-
8a-epi-swainsonine
-
-
alpha-conglutin
-
11% inhibition at saturation
-
alpha-D-1,2-mannopyranosyl-mannopyranose
-
50% inhibition at 4 mM
alpha-D-1,3-mannopyranosyl-mannopyranose
-
50% inhibition at 18 mM
-
alpha-D-1,6-mannopyranosyl-mannopyranose
-
50% inhibition at 100 mM
alpha-D-mannopyranose
cysteine
-
31% inhibition at 0.02 mM
D-glucose
-
67% inhibition at 30 mM
D-glucuronic acid
-
slight inhibition at 10 mM
D-mannono-1,4-lactone
D-Mannono-1,5-lactone
D-mannosylamine
deoxymannojirimycin
diisopropylfluorophosphate
-
42% inhibition at 10 mM
gluco-hydroxyiminolactam
-
-
glucoimidazole
-
-
iodoacetamide
-
slight inhibition at 1 mM
iodoacetic acid
kifunensine
Li+
-
88% inhibition at 20 mM
Mannan
-
33% inhibition at 50 mM
mannoimidazole
-
-
mannose
-
relative inhibition of 67.8%, potent inhibitor, three concentrations of 10, 50, and 100 mM tested
methyl-alpha-D-glucoside
-
23% inhibition at 10 mM
methyl-alpha-D-lyxopyranosyl-(1'-2)-alpha-D-mannopyranoside
-
-
methyl-alpha-D-lyxopyranosyl-(1-2)-alpha-D-mannopyranoside
-
potent
methyl-alpha-D-mannopyranoside
N-bromosuccinimide
-
N-bromosuccinimide modified inactivation, effect of Trp modification on enzyme activity
N-octyl-6-epi-valienamine
-
-
NaN3
-
slight inhibition at 0.1 mM
p-chloromercuribenzenesulfonic acid
p-nitrophenyl-alpha-D-mannopyranoside
-
substrate inhibition greater than 2 mM
SDS
-
85% inhibition at 0.05%, w/v
small interfering RNA
siRNA designed to specifically inhibit Man2C1 gene expression, alpha-mannosidase activity is compromised in siRNA-treated cells. Determination of alteration of siRNA treatment, glycans recovered from membrane glycoproteins are fluorescence-labelled and analysed by HPLC
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Sr2+
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inhibition at 1 mM
swainsonine
Tris
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inhibitory for alpha-mannosidases IA and IB
ZnCl2
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92% inhibition by 1 mM, 56% inhibition by 0.01 mM
[6-(3-methylbutoxy)-3-oxo-3,6-dihydro-2H-pyran-2-yl]acetonitrile
-
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[6-(3-methylbutoxy)-3-oxo-3,6-dihydro-2H-pyran-2-yl]methyl 2,2-dimethylpropanoate
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[[(3S,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)piperidin-2-ylidene]amino] N-(4-chlorophenyl)carbamate
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additional information