Literature summary for 3.2.1.187 extracted from

Nakamura, M.; Yasukawa, Y.; Furusawa, A.; Fuchiwaki, T.; Honda, T.; Okamura, Y.; Fujita, K.; Iwai, H.
Functional characterization of unique enzymes in Xanthomonas euvesicatoria related to degradation of arabinofurano-oligosaccharides on hydroxyproline-rich glycoproteins (2018), PLoS ONE, 13, e0201982 .

Search for more literature for 3.2.1.187

Cloned(Commentary)

Cloned (Comment)	Organism
gene XCV2729, belongs to operon xehypBA2-AA containing genes XCV2724, XCV2728, and XCV2729, DNA and amino acid sequence determination and analysis, and genetic structure. The promoter region of the operon (xehypBA2 and xehypAA) contains the plant-inducible promoter box for binding the regulator protein HrpX involved in pathogenicity, recombinant expression of wild-type and mutant enzymes without the signal peptide and fused with the trigger factor chaperone in Escherichia coli strain BL21(DE3), recombinant expression of the operon in transgenic Solanum lycopersicum strain Micro-Tom	Xanthomonas euvesicatoria

Cloned (Comment)

Organism

gene XCV2729, belongs to operon xehypBA2-AA containing genes XCV2724, XCV2728, and XCV2729, DNA and amino acid sequence determination and analysis, and genetic structure. The promoter region of the operon (xehypBA2 and xehypAA) contains the plant-inducible promoter box for binding the regulator protein HrpX involved in pathogenicity, recombinant expression of wild-type and mutant enzymes without the signal peptide and fused with the trigger factor chaperone in Escherichia coli strain BL21(DE3), recombinant expression of the operon in transgenic Solanum lycopersicum strain Micro-Tom

Xanthomonas euvesicatoria

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a single-gene deletion mutant, DELTAxehypBA2 and a triple-gene deletion mutant DELTAxehypBA1-BA2-AA. The mutants remain pathogenic, and mutations of the operon or the single genes XCV2724, XCV2728, and XCV2729 have no effect on nonhost resistance, either, indicating that these three enzymes are not involved in either pathogenicity or nonhost resistance reactions	Xanthomonas euvesicatoria

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	the enzyme contains a 42 amino-acid signal peptide	Xanthomonas euvesicatoria	-	-

Organism

Organism	UniProt	Comment	Textmining
Xanthomonas euvesicatoria	Q3BS03	-	-
Xanthomonas euvesicatoria 85-10	Q3BS03	-	-
Xanthomonas euvesicatoria UPB139	Q3BS03	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O	-	Xanthomonas euvesicatoria	4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose	-	?
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O	-	Xanthomonas euvesicatoria 85-10	4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose	-	?
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O	-	Xanthomonas euvesicatoria UPB139	4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose	-	?
additional information	substrate specificity, overview. Enzyme XeHypBA2 releases the disaccharide Araf-beta1,2-Araf from Araf-beta1,2-Araf-beta1,2-Araf-beta-Hyp (Ara3-Hyp)	Xanthomonas euvesicatoria	?	-	?
additional information	substrate specificity, overview. Enzyme XeHypBA2 releases the disaccharide Araf-beta1,2-Araf from Araf-beta1,2-Araf-beta1,2-Araf-beta-Hyp (Ara3-Hyp)	Xanthomonas euvesicatoria 85-10	?	-	?
additional information	substrate specificity, overview. Enzyme XeHypBA2 releases the disaccharide Araf-beta1,2-Araf from Araf-beta1,2-Araf-beta1,2-Araf-beta-Hyp (Ara3-Hyp)	Xanthomonas euvesicatoria UPB139	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 152000, recombinant untagged enzyme, SDS-PAGE	Xanthomonas euvesicatoria

Synonyms

Synonyms	Comment	Organism
GH 121 beta-L-arabinofuranosidase	-	Xanthomonas euvesicatoria
GH121 beta-L-arabinobiosidase	-	Xanthomonas euvesicatoria
xcv2729	-	Xanthomonas euvesicatoria
XeHypBA2	-	Xanthomonas euvesicatoria

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Xanthomonas euvesicatoria

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	assay at	Xanthomonas euvesicatoria

Expression

Organism	Comment	Expression
Xanthomonas euvesicatoria	the operon is upregulated by transcription factor HrpX	up

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the glycosyl hydrolase family 121, GH121	Xanthomonas euvesicatoria
malfunction	single deletion mutants of genes XCV2724, XCV2728, and XCV2729 and the triple deletion mutant remain pathogenic, and mutations of the operon or the single genes have no effect on nonhost resistance, indicating that the enzyme is not involved in either pathogenicity or nonhost resistance reactions	Xanthomonas euvesicatoria
physiological function	enzymes XCV2724, XCV2728, and XCV2729 in Xanthomonas euvesicatoria degrade the arabinofuranooligosaccharides present on hydroxyproline (Hyp)-rich glycoproteins (HRGPs) such as extensin and solanaceous lectins in plant cell walls. These enzymes work coordinately to degrade the oligosaccharides. The main oligosaccharide structure of Ara4-Hyp on the HRGPs is degraded to Ara3-Hyp, then to Ara-Hyp, and finally to Ara monosaccharides by the action of these three enzymes. Structure of arabino-oligosaccharides on extensin and solanaceous lectins in plant cell walls, overview. HRGPs containing oligosaccharide substrates have been reported to contribute to plant defense. The enzyme are not involved in either pathogenicity or nonhost resistance reactions	Xanthomonas euvesicatoria