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Information on EC 3.2.1.187 - (Ara-f)3-Hyp beta-L-arabinobiosidase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Bifidobacterium longum
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(Ara-f)3-Hyp beta-L-arabinobiosidase
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4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O = 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
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4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose hydrolase
The enzyme, which was identified in the bacterium Bifidobacterium longum JCM1217, is specific for (Ara-f)3-Hyp, a sugar chain found in hydroxyproline-rich glyoproteins such as extensin and lectin. The enzyme was not able to accept (Ara-f)2-Hyp or (Ara-f)4-Hyp as substrates. In the presence of 1-alkanols, the enzyme demonstrates transglycosylation activity, retaining the anomeric configuration of the arabinofuranose residue.
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beta-L-arabinofuranosidase
beta-L-arabinobiosidase
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beta-L-arabinobiosidase
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beta-L-arabinobiosidase
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beta-L-arabinofuranosidase
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beta-L-arabinofuranosidase
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hypBA2
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gene name
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brenda
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brenda
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metabolism
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the enzyme plays a key role in Bifidobacterium longum for beta-L-arabinooligosaccharides usage
metabolism
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the enzyme plays a key role in Bifidobacterium longum for beta-L-arabinooligosaccharides usage
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4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O
4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
debranched arabinan + H2O
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potato lectin + H2O
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additional information
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4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O
4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
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?
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O
4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
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specific substrate
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?
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O
4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
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specific substrate
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4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O
4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
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arabinan + H2O
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arabinan + H2O
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carrot extension + H2O
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carrot extension + H2O
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debranched arabinan + H2O
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debranched arabinan + H2O
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additional information
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no activity with 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, arabinoxylan, rhamnogalacturonan, 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline and 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(1->2)-(beta-L-arabinofuranosyl)-(1->2)-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline
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additional information
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no activity with 4-nitrophenyl alpha-L-arabinofuranoside, 4-nitrophenyl alpha-L-arabinopyranoside, 4-nitrophenyl beta-L-arabinopyranoside, arabinoxylan, rhamnogalacturonan, 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline and 4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(1->2)-(beta-L-arabinofuranosyl)-(1->2)-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline
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4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O
4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O
4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
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4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O
4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
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specific substrate
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4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O
4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
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specific substrate
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4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O
4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
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Ca2+
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1 mM Ca2+ increases the enzymatic activity by 43.8%
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EDTA
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1 mM EDTA decreases the enzymatic activity to 1.4%
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0.0107
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline
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in 50 mM sodium acetate (pH 6.0), at 37°C
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2.7
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline
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in 50 mM sodium acetate (pH 6.0), at 37°C
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Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
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156797
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x * 156797, calculated from amino acid sequence
157000
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x * 157000, SDS-PAGE
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x * 156797, calculated from amino acid sequence; x * 157000, SDS-PAGE
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x * 156797, calculated from amino acid sequence; x * 157000, SDS-PAGE
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TALON metal affinity resin column chromatography
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expressed in Escherichia coli BL21(lambdaDE3) cells
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HYBA2_BIFL2
Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
1943
210438
Swiss-Prot
A0A100J0V5_9ACTN
1086
118833
TrEMBL
A0A0P0GUJ4_9BACE
1107
127453
TrEMBL
A0A1J5SU04_9ZZZZ
521
59472
TrEMBL
A0A1Q2Z5J8_9ACTN
1086
118822
TrEMBL
A0A142Y315_9PLAN
856
96849
TrEMBL
A0A1E7RVH6_BUTME
1816
194602
TrEMBL
A0A0P0FQG4_BACT4
1109
127331
TrEMBL
A0A1V6GB99_9BACT
733
78553
TrEMBL
A0A143ZUW0_9FIRM
1913
211715
TrEMBL
A0A1V5NE29_9BACT
661
71787
TrEMBL
A0A1B2U3Z5_FLAJO
522
61220
TrEMBL
A0A1V6EM15_9BACT
527
61595
TrEMBL
A0A1E3AWW9_9FIRM
688
80168
TrEMBL
A0A1V6CWH8_9BACT
1104
126432
TrEMBL
A0A120A4V0_9BACE
1106
126865
TrEMBL
A0A163R0V7_9CELL
1177
121332
TrEMBL
A0A0M2HAP4_9MICO
1572
167618
TrEMBL
A0A1V6GVF0_9BACT
1191
129089
TrEMBL
A0A1V6GKA2_9BACT
421
46398
TrEMBL
A0A143XBR7_9ACTN
756
79021
TrEMBL
A0A161XDE3_9CELL
1522
162479
TrEMBL
A0A163SCX6_9CELL
655
67372
TrEMBL
A0A0M2HB15_9MICO
658
68143
TrEMBL
A0A1E3AAY6_9FIRM
688
80207
TrEMBL
Q8A8Z7_BACTN
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
1109
127331
TrEMBL
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Fujita, K.; Sakamoto, S.; Ono, Y.; Wakao, M.; Suda, Y.; Kitahara, K.; Suganuma, T.
Molecular cloning and characterization of a beta-L-arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family
J. Biol. Chem.
286
5143-5150
2011
Bifidobacterium longum, Bifidobacterium longum JCM 1217
brenda
Fujita, K.; Takashi, Y.; Obuchi, E.; Kitahara, K.; Suganuma, T.
Characterization of a novel beta-L-arabinofuranosidase in Bifidobacterium longum: functional elucidation of a DUF1680 family member
J. Biol. Chem.
289
5240-5249
2014
Bifidobacterium longum, Bifidobacterium longum JCM 1217
brenda
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