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Literature summary for 3.2.1.18 extracted from
- Active 1918 pandemic flu viral neuraminidase has distinct N-glycan profile and is resistant to trypsin digestion (2009), Biochem. Biophys. Res. Commun., 379, 749-753.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in SF21 cells | influenza A virus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
plus dimer and monomer, only the tetramer is enzymatically active. 4 * 55000, SDS-PAGE. Molecular mass is slightly higher than that of monomer and dimer, and only tetramer is resistant to trypsin digestion | influenza A virus |
| 55000 | - |
plus dimer and tetramer, only the tetramer is enzymatically active. Monomer and dimer cannot be oligomerized to the tetramer. 1 * 55000, SDS-PAGE | influenza A virus |
| 55000 | - |
plus monomer and tetramer, only the tetramer is enzymatically active. Monomer and dimer cannot be oligomerized to the tetramer. 2 * 55000, SDS-PAGE | influenza A virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| influenza A virus | Q9IGQ6 | - |
- |
| influenza A virus A/South Carolina/1/1918 H1N1 | Q9IGQ6 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | deglycosylation of monomer, dimer or tetramer with PNGase F results in molecular masses of 48000 Da. Glycosylation of tetramer is different from monomer and dimer | influenza A virus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | influenza A virus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | plus monomer and tetramer, only the tetramer is enzymatically active. Monomer and dimer cannot be oligomerized to the tetramer. 2 * 55000, SDS-PAGE | influenza A virus |
| monomer | plus dimer and tetramer, only the tetramer is enzymatically active. Monomer and dimer cannot be oligomerized to the tetramer. 1 * 55000, SDS-PAGE | influenza A virus |
| tetramer | plus dimer and monomer, only the tetramer is enzymatically active. 4 * 55000, SDS-PAGE. Molecular mass is slightly higher than that of monomer and dimer, and only tetramer is resistant to trypsin digestion | influenza A virus |
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