Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lilium longiflorum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme is purified by ammonium sulfate precipitation, DEAE-Sephacel chromatography, sephacryl S-200 gel filtration, octyl sepharose CL-4B chromatography and superdex 200 gel filtration | Lilium longiflorum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Lilium longiflorum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Man-alpha-(1-3)Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O | relative hydrolysis rate: 42% | Lilium longiflorum | GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-3)Man-alpha-(1-6)Man | - |
? | |
Man-alpha-(1-6)(Man-alpha-(1-3))Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O | relative hydrolysis rate: 34% | Lilium longiflorum | GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)(Man-alpha-(1-3))Man-alpha-(1-6)Man | - |
? | |
Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O | relative hydrolysis rate: 100% | Lilium longiflorum | GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)Man | - |
? | |
Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-pyridylamine + H2O | relative hydrolysis rate: 9% | Lilium longiflorum | D-mannose + GlcNAc-beta-(1->4)-GlcNAc-beta-pyridylamine | - |
? | |
additional information | EBM II has both endo-beta-mannosidase activity and alpha 1,2-L-fucosidase activity | Lilium longiflorum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | SDS-PAGE of the purified protein shows that EBM II is comprised of six polypeptides with molecular masses of 65000, 46000, 42000, 31000, 28000 and 27000 Da. Analysis of the fragments disclose EBM II consists of endo-beta-mannosidase and an associated protein that has slight homology to the fucosidase domain of the bifidus AfcA protein | Lilium longiflorum |
Synonyms | Comment | Organism |
---|---|---|
EBM II | - |
Lilium longiflorum |
endo-beta-mannosidase | - |
Lilium longiflorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Lilium longiflorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Lilium longiflorum |