Information on EC 3.2.1.152 - mannosylglycoprotein endo-beta-mannosidase

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The expected taxonomic range for this enzyme is: Magnoliophyta

EC NUMBER
COMMENTARY
3.2.1.152
-
RECOMMENDED NAME
GeneOntology No.
mannosylglycoprotein endo-beta-mannosidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Hydrolysis of the alpha-D-mannosyl-(1->6)-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl sequence of glycoprotein to alpha-D-mannosyl-(1->6)-D-mannose and N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl sequences
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis
-, Q75W54
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
endo-beta-mannosidase
Q75W54
-
endo-beta-mannosidase
-
-
endo-beta-mannosidase
-
-
endo-beta-mannosidase
Q5H7P5
-
CAS REGISTRY NUMBER
COMMENTARY
141176-95-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Thunb. cv Hinomoto
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Man)nMan-alpha-(1-6)-Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-peptide + pyridylamino-GlcNAc-beta-(1-4)GlcNAc
pyridylamino-(Man)nMan-alpha-(1-6)-Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc + GlcNAc-beta-(1-4)GlcNAc-peptide
show the reaction diagram
-
n = 0-2
-
-
?
Man-alpha(1-6)[Man-alpha(1-3)]Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamide + H2O
Man-alpha(1-6)[Man-alpha(1-3)]Man-alpha-(1-6)Man + GlcNAc-beta-(1-4)GlcNAc-pyridylamide
show the reaction diagram
-
-
-
-
?
Man-alpha-(1-3)Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O
GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-3)Man-alpha-(1-6)Man
show the reaction diagram
-
relative hydrolysis rate: 42%
-
-
?
Man-alpha-(1-6)(Man-alpha-(1-3))Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O
GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)(Man-alpha-(1-3))Man-alpha-(1-6)Man
show the reaction diagram
-
relative hydrolysis rate: 34%
-
-
?
Man-alpha-(1-6)Man-alpha(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamide + H2O
Man-alpha-(1-6)Man-alpha(1-6)Man + GlcNAc-beta-(1-4)GlcNAc-pyridylamide
show the reaction diagram
-
-
-
-
?
Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamide + H2O
Man-alpha-(1-6)Man + GlcNAc-beta-(1-4)GlcNAc-pyridylamide
show the reaction diagram
-
-
-
-
?
Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O
GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)Man
show the reaction diagram
-
-
-
-
?
Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O
GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)Man
show the reaction diagram
-
-
-
-
ir
Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O
GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)Man
show the reaction diagram
-, Q75W54
-
-
-
?
Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O
GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)Man
show the reaction diagram
Q5H7P5
-
-
-
?
Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O
GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)Man
show the reaction diagram
-
relative hydrolysis rate: 100%
-
-
?
Man-beta-(1-4)GlcNAc + H2O
?
show the reaction diagram
-
hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans
-
-
?
Man-beta-(1-4)GlcNAc + H2O
?
show the reaction diagram
Q75W54
hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans
-
-
?
Man-beta-(1-4)GlcNAc + H2O
?
show the reaction diagram
Q5H7P5
hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans
-
-
?
Man-beta-(1-4)GlcNAc + H2O
?
show the reaction diagram
-
hydrolysis of Man-beta-(1-4)GlcNAc linkage of N-linked sugar chains
-
-
ir
Man-beta-(1-4)GlcNAc + H2O
?
show the reaction diagram
-
hydrolysis of Man-beta-(1-4)GlcNAc linkage of N-linked sugar chains, possible role in the catabolism of N-linked sugar chains leading to oligosaccharides, hydrolysis of Man-beta-1-4GlcNAc linkage of N-linked sugar chains
-
-
?
Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-peptide + p-nitrophenyl-beta-D-glucoside
Man-beta-(1-4)GlcNAc-beta-(1-4)Glc-beta-1-p-nitrophenyl + GlcNAc-peptide
show the reaction diagram
-
-
-
-
?
Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-peptide + p-nitrophenyl-beta-D-mannoside
Man-beta-(1-4)GlcNAc-beta-1-p-nitrophenyl-beta-D-mannoside + GlcNAc-peptide
show the reaction diagram
-
-
-
-
?
Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-peptide + p-nitrophenyl-beta-N-acetylglucosaminide
Man-beta-(1-4)GlcNAc-beta-1-p-nitrophenyl-beta-N-acetylglucosaminide + GlcNAc-peptide
show the reaction diagram
-
-
-
-
?
Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc-beta-pyridylamine + H2O
D-mannose + GlcNAc-beta-(1->4)-GlcNAc-beta-pyridylamine
show the reaction diagram
-
relative hydrolysis rate: 9%
-
-
?
Man-beta-1-4GlcNAc + H2O
?
show the reaction diagram
-
hydrolysis of Man-beta-1-4GlcNAc linkage of N-linked sugar chains
-
-
ir
Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)Man + GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
show the reaction diagram
-
40% activity relative to M2B-PA
-
-
?
Manalpha(1-3)[Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
Manalpha(1-3)[Manalpha(1-6)]Man + GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
show the reaction diagram
-
34% activity relative to Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
-
-
?
Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
Manalpha(1-6)Man + GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
show the reaction diagram
-
100% activity
-
-
?
Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O
beta-D-mannose + GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
show the reaction diagram
-
2.6% activity relative to M2B-PA
-
-
?
additional information
?
-
-
enzyme does not hydrolyze beta-(1-4)-mannohexaose or p-nitrophenyl-beta-mannopyranoside
-
-
-
additional information
?
-
-
enzyme does not hydrolyze pyridylamino-sugar chains containing Man-alpha-(1-3)Man-beta or Xylose-beta-(1-2)Man-beta, enzyme does not hydrolyze p-nitrophenyl-beta-mannoside
-
-
-
additional information
?
-
Q75W54
enzyme does not hydrolyze pyridylamino-sugar chains containing Man-alpha-(1-3)Man-beta or Xylose-beta-(1-2)Man-beta, enzyme does not hydrolyze p-nitrophenyl-beta-mannoside
-
-
-
additional information
?
-
Q5H7P5
enzyme does not hydrolyze pyridylamino-sugar chains containing Man-alpha-(1-3)Man-beta or Xylose-beta-(1-2)Man-beta, enzyme does not hydrolyze p-nitrophenyl-beta-mannoside
-
-
-
additional information
?
-
-
p-nitrophenyl-beta-N-acetylgalactosaminide does not work as acceptor in the transfer reaction
-
-
-
additional information
?
-
-
EBM II has both endo-beta-mannosidase activity and alpha 1,2-L-fucosidase activity
-
-
-
additional information
?
-
-
Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, Manalpha(1-3)[Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)[(Manalpha(1-3))]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)]Manalpha(1-6)[Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, GlcNAcbeta(1-4)Manalpha(1-3)[GlyNAcbeta(1-4)Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside, Manalpha(1-6)[Xylbeta(1-2)Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))]GlcNAc-pyridylaminoside, p-nitrophenyl alpha-mannoside, p-nitrophenyl beta-mannoside, and Manbeta-(1-4)Manbeta(1-4)Man-beta-(1-4)Manbeta(1-4)Man-beta(1-4)Man are no substrates
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Man-beta-(1-4)GlcNAc + H2O
?
show the reaction diagram
-
hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans
-
-
?
Man-beta-(1-4)GlcNAc + H2O
?
show the reaction diagram
Q75W54
hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans
-
-
?
Man-beta-(1-4)GlcNAc + H2O
?
show the reaction diagram
Q5H7P5
hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans
-
-
?
Man-beta-(1-4)GlcNAc + H2O
?
show the reaction diagram
-
hydrolysis of Man-beta-(1-4)GlcNAc linkage of N-linked sugar chains
-
-
ir
Man-beta-(1-4)GlcNAc + H2O
?
show the reaction diagram
-
hydrolysis of Man-beta-(1-4)GlcNAc linkage of N-linked sugar chains, possible role in the catabolism of N-linked sugar chains leading to oligosaccharides
-
-
?
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.2
-
Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine
Q5H7P5
pH 5.0, 37C
1.4
-
Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine
-
pH 5.0, 37C
0.54
-
Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside
-
in ammonium acetate buffer, pH 5.0, at 37C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.004
-
-
purified enzyme, pH 5.0, 37C
0.026
-
-, Q75W54
purified enzyme, pH 5.0, 37C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
-
Q5H7P5
-
5
-
-, Q75W54
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
6
Q5H7P5
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Q5H7P5
highest activity
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
78000
-
-
gel filtration
108000
-
Q5H7P5
calculated from the deduced amino acid sequence
112000
-
-, Q75W54
-
141000
-
-
SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
tetramer
-
1 * 42000 + 1 * 34000 + 1 * 28000 + 1 * 10000, gel filtration
trimer
Q5H7P5
alphabetagamma, 1 * 42000 + 1 * 31000 + 1 * 28000, SDS-PAGE
?
-
three bands on SDS-PAGE of 42000, 31000, and 28000 Da
additional information
-
SDS-PAGE of the purified protein shows that EBM II is comprised of six polypeptides with molecular masses of 65000, 46000, 42000, 31000, 28000 and 27000 Da. Analysis of the fragments disclose EBM II consists of endo-beta-mannosidase and an associated protein that has slight homology to the fucosidase domain of the bifidus AfcA protein
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
proteolytic modification
Q5H7P5
enzyme is encoded by a single cDNA, translation product is posttranslationally processed by proteolysis
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3
6
-
relatively stable at pH 3-6
3
7
-, Q75W54
unstable below pH 3 and above pH 7
3
8
Q5H7P5
unstable below pH 3 and above pH 8
additional information
-
-
unstable at alkaline pH
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial purification of the recombinant protein
-, Q75W54
; ammonium sulfate precipitation, Poros HS column chromatography, hydroxyapatite chromatography, DEAE-Sephacel column chromatography, Octyl Sepharose CL-4B column chromatography, and Superdex 200 gel filtration
-
enzyme is purified by ammonium sulfate precipitation, DEAE-Sephacel chromatography, sephacryl S-200 gel filtration, octyl sepharose CL-4B chromatography and superdex 200 gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli RosettaBlue
-, Q75W54
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
E464A
-, Q75W54
no activity
E549A
-, Q75W54
no activity
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
synthesis
-
possible application in the synthesis of oligosaccharides containing mannosyl-beta-1,4-structures