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Literature summary for 3.2.1.151 extracted from
- A novel xyloglucan-specific endo-beta-1,4-glucanase: Biochemical properties and inhibition studies (2010), Appl. Microbiol. Biotechnol., 86, 1463-1471.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3)pLysE cells | uncultured bacterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| XXXG | the addition of XXXG results in linear noncompetitive inhibition on the hydrolysis of tamarind xyloglucan | uncultured bacterium |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45455 | - |
x * 45455, calculated from amino acid sequence | uncultured bacterium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | D2K7Z0 | from cow's rumen | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | - |
uncultured bacterium | ? | - |
? | |
| lichenan + H2O | - |
uncultured bacterium | ? | - |
? | |
| additional information | the enzyme is devoid of transglycosylase activities. There is undetectable activity on cellulose, xylan, arbinoxylan, laminarin, or pectin | uncultured bacterium | ? | - |
? | |
| tamarind xyloglucan + H2O | high specific activity | uncultured bacterium | ? | - |
? | |
| xyloglucan + H2O | the enzyme catalyzes endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan | uncultured bacterium | xyloglucan oligosaccharides | - |
? | |
| xyloglucan oligosaccharide HDP-XGO + H2O | - |
uncultured bacterium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45455, calculated from amino acid sequence | uncultured bacterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-xyloglucanase | - |
uncultured bacterium |
| XEG5A | - |
uncultured bacterium |
| xyloglucan-specific endo-beta-1,4-glucanase | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | - |
uncultured bacterium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | the enzyme is stable up to 50°C. The enzyme is completely inactivated by incubation at 70°C for 10 min | uncultured bacterium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.24 | - |
tamarind xyloglucan | at 37°C, pH 7.0 | uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
uncultured bacterium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
the enzyme is relatively stable at pH above 5.0, where the enzyme retains more than 95% of the activity | uncultured bacterium |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.46 | - |
XXXG | pH and temperature not specified in the publication | uncultured bacterium | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| uncultured bacterium | calculated from amino acid sequence | - |
4.5 |
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