Application | Comment | Organism |
---|---|---|
synthesis | mutant engineered xyloglucanases acting as glycosynthases are emerging as useful tools for the synthesis of large, complex polysaccharides, method development for robust and versatile method for the preparative synthesis of homogeneous xyloglucans with regular substitution patterns not available in nature, overview | Bacillus licheniformis |
synthesis | mutant engineered xyloglucanases acting as glycosynthases are emerging as useful tools for the synthesis of large, complex polysaccharides, method development for robust and versatile method for the preparative synthesis of homogeneous xyloglucans with regular substitution patterns not available in nature, overview | Tropaeolum majus |
Protein Variants | Comment | Organism |
---|---|---|
E155A | the mutant acts as glucosynthase and can perform the condensation of xyloglucosyl fluorides, albeit at poor rates | Bacillus licheniformis |
E94A | the GH16 xyloglucan hydrolase mutant of TmNXG1 acting as glycosynthase is capable of synthesizing XLLG-based xyloglucan oligosaccharides at rates feasible for preparative synthesis, thus providing an essential expansion of product ranges | Tropaeolum majus |
additional information | mutation and engineering of wild-type xyloglucanase to glycosynthases, hydrolytically inactive mutant glycosidases that catalyze glycosylation reactions using glycosyl fluoride donor substrates | Bacillus licheniformis |
additional information | mutation and engineering of wild-type xyloglucanase to glycosynthases, hydrolytically inactive mutant glycosidases that catalyze glycosylation reactions using glycosyl fluoride donor substrates | Tropaeolum majus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Tropaeolum majus | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
BlXG12 | - |
Bacillus licheniformis |
GH12 xyloglucanase | - |
Bacillus licheniformis |
GH16 xyloglucan hydrolase | - |
Tropaeolum majus |
TmNXG1 | - |
Tropaeolum majus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at, mutant enzyme | Bacillus licheniformis |
30 | - |
assay at, mutant enzyme | Tropaeolum majus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at, mutant enzyme | Tropaeolum majus |
6 | - |
assay at, mutant enzyme | Bacillus licheniformis |