Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Acetivibrio thermocellus |
Crystallization (Comment) | Organism |
---|---|
unliganded wild-type and mutant D70A in complex with oligosaccharide substrate | Acetivibrio thermocellus |
Protein Variants | Comment | Organism |
---|---|---|
D74A | crystallization data in complex with oligosaccharide substrate | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values are 0.76 mg/ml for xyloglucan, 4.6 mg/ml for carboxymethyl cellulose, 6.6 mg/ml for lichenan, 5.1 mg/ml for carboxyethyl cellulose | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
isoform XGH74A | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxyethyl cellulose + H2O | - |
Acetivibrio thermocellus | carboxyethyl cellulose oligosaccharides | - |
? | |
carboxymethyl cellulose + H2O | carboxymethyl cellulose-4M | Acetivibrio thermocellus | carboxymethyl cellulose oligosaccharides | - |
? | |
lichenan + H2O | - |
Acetivibrio thermocellus | lichenan cellulose oligosaccharides | - |
? | |
additional information | enzyme does not cleave at substituted glucosyl moieties | Acetivibrio thermocellus | ? | - |
? | |
xyloglucan + H2O | source of substrate tamarind seed | Acetivibrio thermocellus | xyloglucan oligosaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XGH74 | - |
Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
carboxyethyl cellulose | pH 7.0, 50°C | Acetivibrio thermocellus | |
0.59 | - |
lichenan | pH 7.0, 50°C | Acetivibrio thermocellus | |
0.72 | - |
carboxymethyl cellulose | pH 7.0, 50°C | Acetivibrio thermocellus | |
0.78 | - |
xyloglucan | pH 7.0, 50°C | Acetivibrio thermocellus |