Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | complete inhibition at 1 mM | Aspergillus sojae | |
additional information | not affected by 2-mercaptoethanol, 15% (v/v) ethanol or 100 mg/l SO2 | Aspergillus sojae | |
trigalacturonate | - |
Aspergillus sojae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by Ca2+, K+, Mg2+, and Zn2+ | Aspergillus sojae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus sojae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography, SP Sepharose column chromatography, and Superdex 75 gel filtration | Aspergillus sojae |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
citrus pectin + H2O | - |
Aspergillus sojae | ? | - |
? | |
lemon protopectin + H2O | - |
Aspergillus sojae | ? | - |
? | |
polygalacturonate + H2O | - |
Aspergillus sojae | trigalacturonate + digalactuornate + D-galacturonic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE | Aspergillus sojae |
Synonyms | Comment | Organism |
---|---|---|
endo-polygalacturonase | - |
Aspergillus sojae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme presents a half-life of 2 h at 50°C. The enzyme is fully thermostable up to 45°C, whereas 25% of the initial activity is retained after 210 min of incubation at 50°C | Aspergillus sojae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
with citrus pectin as substrate | Aspergillus sojae |
4.3 | - |
with lemon protopectin as substrate | Aspergillus sojae |
5 | - |
with polygalacturonate as substrate | Aspergillus sojae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 6.5 | after 2 h of incubation, the purified enzyme retains more than 75% of its initial activity between the pH values of 2.0 and 6.5, and shows up to 55% residual activity within a very low pH interval (1.0-2.0). After 24 h in the pH range of 1.0-3.0 the enzyme retains 35% of its initial activity at pH 1.0 and more than 75% from pH 1.5 onwards | Aspergillus sojae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
trigalacturonate | at pH 5.0 and 20°C | Aspergillus sojae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus sojae | isoelectric focusing | - |
4.2 |