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Literature summary for 3.2.1.15 extracted from
- Immobilization of an alkaline endopolygalacturonase purified from Bacillus paralicheniformis exhibits bioscouring of cotton fabrics (2018), Bioprocess Biosyst. Eng., 41, 1425-1436 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 131.6% activity at 5 mM | Bacillus paralicheniformis |  |
| hydrogen peroxide | 143.4% activity at 5 mM | Bacillus paralicheniformis | |
| sodium perborate | 134.1% activity at 5 mM | Bacillus paralicheniformis | |
Application
| Application | Comment | Organism |
|---|---|---|
| industry | the enzyme is developed a feasible strategy to achieve a rapid benign of the bioscouring process with the wettability properties of cotton fabrics | Bacillus paralicheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 91.8% residual activity at 5 mM | Bacillus paralicheniformis | |
| CHAPS | 76.1% residual activity at 0.25% (v/v) | Bacillus paralicheniformis | |
| Co2+ | 98.4% residual activity at 5 mM | Bacillus paralicheniformis | |
| EDTA | 55.7% residual activity at 2 mM | Bacillus paralicheniformis | |
| EGTA | 76.4% residual activity at 2 mM | Bacillus paralicheniformis | |
| Mg2+ | 85.3% residual activity at 5 mM | Bacillus paralicheniformis | |
| Ni2+ | 91.5% residual activity at 5 mM | Bacillus paralicheniformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 130.1% activity at 5 mM | Bacillus paralicheniformis | |
| Cu2+ | 134.4% activity at 5 mM | Bacillus paralicheniformis | |
| Fe2+ | 106.1% activity at 5 mM | Bacillus paralicheniformis | |
| K+ | 148.2% activity at 5 mM | Bacillus paralicheniformis | |
| Mg2+ | 112.5% activity at 5 mM | Bacillus paralicheniformis | |
| Na+ | 150.2% activity at 5 mM | Bacillus paralicheniformis | |
| Zn2+ | 123.1% activity at 5 mM | Bacillus paralicheniformis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus paralicheniformis | - |
- |
- |
| Bacillus paralicheniformis CBS3 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Sepharose CL 6B column chromatography, and DEAE Sepharose column chromatography | Bacillus paralicheniformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 100.56 | - |
crude enzyme, at pH 9.1 and 60°C | Bacillus paralicheniformis |
| 2216.4 | - |
after 22.04fold purification, at pH 9.1 and 60°C | Bacillus paralicheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| apple pectin + H2O | 100% activity | Bacillus paralicheniformis | digalacturonic acid | - |
? | |
| apple pectin + H2O | 100% activity | Bacillus paralicheniformis CBS3 | digalacturonic acid | - |
? | |
| banana peel pectin + H2O | 25.8% activity compared to apple pectin | Bacillus paralicheniformis | digalacturonic acid | - |
? | |
| banana peel pectin + H2O | 25.8% activity compared to apple pectin | Bacillus paralicheniformis CBS3 | digalacturonic acid | - |
? | |
| carrot peel pectin + H2O | 12.4% activity compared to apple pectin | Bacillus paralicheniformis | digalacturonic acid | - |
? | |
| carrot peel pectin + H2O | 12.4% activity compared to apple pectin | Bacillus paralicheniformis CBS3 | digalacturonic acid | - |
? | |
| citrus peel pectin + H2O | 85.2% activity compared to apple pectin | Bacillus paralicheniformis | digalacturonic acid | - |
? | |
| citrus peel pectin + H2O | 85.2% activity compared to apple pectin | Bacillus paralicheniformis CBS3 | digalacturonic acid | - |
? | |
| orange peel pectin + H2O | 16.2% activity compared to apple pectin | Bacillus paralicheniformis | digalacturonic acid | - |
? | |
| orange peel pectin + H2O | 16.2% activity compared to apple pectin | Bacillus paralicheniformis CBS3 | digalacturonic acid | - |
? | |
| polygalacturonate + H2O | 71.5% activity compared to apple pectin | Bacillus paralicheniformis | digalacturonic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE | Bacillus paralicheniformis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BPN3 | - |
Bacillus paralicheniformis |
| endopolygalacturonase | - |
Bacillus paralicheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
immobilized enzyme | Bacillus paralicheniformis |
| 60 | - |
free enzyme | Bacillus paralicheniformis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | more than 40% activity between 40 and 70°C, about 80% activity at 50°C | Bacillus paralicheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | the enzyme is thermally stable at 50-60°C for 1 h | Bacillus paralicheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.1 | - |
- |
Bacillus paralicheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | 11.5 | more than 90% activity between pH 8.1 and 11.5 | Bacillus paralicheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | 11.5 | the enzyme reserves over 65% of the initial activity at a pH range of 8.1-11.5 after incubating the sample at 4°C for 24 h | Bacillus paralicheniformis |
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Release 2023.1 (January 2023)
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