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Literature summary for 3.2.1.144 extracted from
- alpha-KDOase activity in oyster and synthesis of alpha- and beta-4-methylumbelliferyl ketosides of 3-deoxy-D-manno-octulosonic acid (KDO) (1997), J. Biol. Chem., 272, 26419-26424.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| re-lipopolysaccharide + H2O | Crassostrea virginica | - |
3-deoxy-D-manno-octulosonic acid + lipopolysaccharide | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crassostrea virginica | - |
oyster | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephacryl S200, SP-Fractogel, Con A-Sepharose, octyl-Sepharose | Crassostrea virginica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatopancreas | - |
Crassostrea virginica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1433 | - |
- |
Crassostrea virginica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl 3-deoxy-alpha-D-manno-2-octulopyranosidonic acid + H2O | - |
Crassostrea virginica | 3-deoxy-D-manno-octulosonic acid + 4-methylumbelliferone | - |
? | |
| re-lipopolysaccharide + H2O | - |
Crassostrea virginica | 3-deoxy-D-manno-octulosonic acid + lipopolysaccharide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-Kdo-ase | enzyme releases alpha linked 3-deoxy-D-manno-octulosonic acid (KDO) from lipopolysaccharides | Crassostrea virginica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
pH-optimum for natural substrate re-lipopolysaccharide, optimum for 4-methylumbelliferyl 3-deoxy-alpha-D-manno-2-octulopyranosidonic acid at pH 3 | Crassostrea virginica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | - |
Crassostrea virginica |
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