Information on EC 3.2.1.144 - 3-deoxyoctulosonase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Crassostrea virginica

EC NUMBER
COMMENTARY
3.2.1.144
-
RECOMMENDED NAME
GeneOntology No.
3-deoxyoctulosonase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3-deoxyoctulosonyl-lipopolysaccharide + H2O = 3-deoxyoctulosonic acid + lipopolysaccharide
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3-deoxyoctulosonyl-lipopolysaccharide hydrolase
Releases Kdo (alpha- and beta-linked 3-deoxy-D-manno-octulosonic acid) from different lipopolysaccharides, including Re-LPS from Escherichia coli and Salmonella, Rd-LPS from S. minnesota, and de-O-acyl-re-LPS. 4-Methylumbelliferyl-alpha-Kdo (alpha-Kdo-OMec) is also a substrate.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
alpha-Kdo-ase
-
enzyme releases alpha linked 3-deoxy-D-manno-octulosonic acid (KDO) from lipopolysaccharides
CAS REGISTRY NUMBER
COMMENTARY
199128-67-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl 3-deoxy-alpha-D-manno-2-octulopyranosidonic acid + H2O
3-deoxy-D-manno-octulosonic acid + 4-methylumbelliferone
show the reaction diagram
-
-
-
?
re-lipopolysaccharide + H2O
3-deoxy-D-manno-octulosonic acid + lipopolysaccharide
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
re-lipopolysaccharide + H2O
3-deoxy-D-manno-octulosonic acid + lipopolysaccharide
show the reaction diagram
-
-
-
?
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.5
-
-
pH-optimum for natural substrate re-lipopolysaccharide, optimum for 4-methylumbelliferyl 3-deoxy-alpha-D-manno-2-octulopyranosidonic acid at pH 3
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Sephacryl S200, SP-Fractogel, Con A-Sepharose, octyl-Sepharose
-