Protein Variants | Comment | Organism |
---|---|---|
G151D | inactive. Use of strain for isolation of mutant genes with restored activity | Mitsuaria chitosanitabida |
G151D/N222S | mutant with restored activity based on inactive mutant G151D, 1.2 fold higher in specific activity than wild-type and 17% increase in thermal stability at 50°C | Mitsuaria chitosanitabida |
L74Q/V75I/G151D | mutant with restored activity based on inactive mutant G151D, 1.5fold higher in specfic activity than wild-type, and protein is efficiently secreted | Mitsuaria chitosanitabida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mitsuaria chitosanitabida | - |
- |
- |
Mitsuaria chitosanitabida 3001 | - |
- |
- |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Mitsuaria chitosanitabida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
1 h, 25% residual activity for wild-type, 27% residual activity for mutant L74Q/V75I/G151D, 42% residual activity for mutant G151D/N222S | Mitsuaria chitosanitabida |