General Stability | Organism |
---|---|
repeated freezing and thawing, activity declines fast | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phlorizin | - |
Homo sapiens | |
Tris | competitive | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
phlorizin | - |
Homo sapiens | |
4.4 | - |
cellobiose | - |
Homo sapiens | |
21 | - |
lactose | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
2 * 160000, SDS-PAGE | Homo sapiens |
280000 | - |
hydrophilic enzyme form, gel filtration | Homo sapiens |
320000 | - |
amphiphilic enzyme form, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
small intestine | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, stable for several months | Homo sapiens |
4°C, stable for several weeks | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | 14% of the activity with lactose | Homo sapiens | 2 beta-D-glucose | - |
? | |
cellulose + H2O | - |
Homo sapiens | beta-D-glucose | - |
? | |
gentiobiose + H2O | 0.7% of the activity with lactose | Homo sapiens | 2 beta-D-glucose | - |
? | |
lactose + H2O | - |
Homo sapiens | D-glucose + D-galactose | - |
? | |
o-nitrophenyl-beta-galactoside + H2O | 17% of the activity with lactose | Homo sapiens | o-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-galactoside + H2O | 13% of the activity with lactose | Homo sapiens | p-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl-beta-glucoside + H2O | 10% of the activity with lactose | Homo sapiens | p-nitrophenol + beta-D-glucose | - |
? | |
phenyl-beta-galactoside + H2O | 0.1% of the activity with lactose | Homo sapiens | phenol + beta-D-galactose | - |
? | |
phlorizin + H2O | 22% of the activity with lactose | Homo sapiens | phloretin + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 160000, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 6 | hydrolysis of lactose | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | pH 5.0: about 70% of maximal activity, pH 7.0: about 65% of maximal activity | Homo sapiens |