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Literature summary for 3.2.1.103 extracted from
- endo-beta-Galactosidase of Escherichia freundii. Purification and endoglycosidic action on keratan sulfates, oligosaccharides, and blood group active glycoprotein (1976), J. Biol. Chem., 251, 6218-6225.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | enzyme can be useful in studying the precise structures of keratan sulfates, related glycoproteins and oligosaccharides | Citrobacter freundii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Citrobacter freundii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.11 | - |
- |
Citrobacter freundii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gal(beta1-4)GlcNAc(beta1-3)Gal(beta1-4)sorbitol + H2O | - |
Citrobacter freundii | Gal(beta1-4)GlcNAc(beta1-3)Gal + sorbitol | - |
? |  |
| keratan sulfate + H2O | - |
Citrobacter freundii | keratan oligosaccharides | 2-acetamido-2-deoxy-6-O-sulfo-beta-D-Gal-(1-3)-D-Gal + 2-acetamido-2-deoxy-6-O-sulfo-beta-D-Glc-(1-3)-6-O-sulfoD-Gal-(1-4)-2-acetamido-2-deoxy-6-O-sulfo-beta-D-Glc-(1-3)-D-Gal | ? | |
| additional information | AB active blood group substance can be hydrolyzed only after Smith degradation | Citrobacter freundii | ? | - |
? | |
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