Information on EC 3.2.1.103 - keratan-sulfate endo-1,4-beta-galactosidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.2.1.103
-
RECOMMENDED NAME
GeneOntology No.
keratan-sulfate endo-1,4-beta-galactosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Endohydrolysis of (1->4)-beta-D-galactosidic linkages in keratan sulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosaminoglycan degradation
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
keratan-sulfate 4-beta-D-galactanohydrolase
Hydrolyses the 1,4-beta-D-galactosyl linkages adjacent to 1,3-N-acetyl-alpha-D-glucosaminyl residues. Also acts on some non-sulfated oligosaccharides, but only acts on blood group substances when the 1,2-linked fucosyl residues have been removed (cf. EC 3.2.1.102 blood-group-substance endo-1,4-beta-galactosidase).
CAS REGISTRY NUMBER
COMMENTARY hide
55072-01-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Bacillus sp. Ks 36
Ks 36
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Pseudomonas sp. IFO-13309
IFO-13309
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
A1-glycolipid + H2O
?
show the reaction diagram
-
-
-
-
?
Ab-glycolipid + H2O
?
show the reaction diagram
-
-
-
-
?
erythroglycan + H2O
?
show the reaction diagram
-
-
-
-
?
Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
Gal(beta1-4)GlcNAc(beta1-3)Gal(beta1-4)sorbitol + H2O
Gal(beta1-4)GlcNAc(beta1-3)Gal + sorbitol
show the reaction diagram
-
-
-
?
Galalpha1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
?
show the reaction diagram
Galbeta1-3GlcNAc(6-2alphaNeuAc)beta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
Galbeta1-4GlcNAcbeta1-3Galbeta1-3Galbeta1-4Glc-Cer + H2O
Galbeta1-4GlcNAcbeta1-3Galbeta1-3Gal + Glc-Cer
show the reaction diagram
Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
glycosphingolipids + H2O
?
show the reaction diagram
-
lacto-N-glycosylceramide series having the common structure R-GlcAcbeta1-3Galbeta1-4Glc(or GlcNAc) are hydrolyzed at Galbeta1-4Glc (or GlcNAC) linkages. beta-Galactosyl linkages in globo series or ganglio series of glycolipids are not hydrolyzed
-
-
?
H1-glycolipid + H2O
?
show the reaction diagram
-
-
-
-
?
keratan sulfate + H2O
keratan oligosaccharides
show the reaction diagram
lacto-N-fucopentaose I + H2O
?
show the reaction diagram
-
-
-
-
?
lacto-N-fucopentaose II + H2O
?
show the reaction diagram
-
-
-
-
?
lacto-N-fucopentaose III + H2O
?
show the reaction diagram
-
-
-
-
?
lacto-N-neotetraosylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
lacto-N-tetraitol + H2O
sorbitol + Galbeta(1-3)GlcNAcbeta(1-3)Gal
show the reaction diagram
-
-
-
?
lacto-N-tetraose + H2O
?
show the reaction diagram
-
-
-
-
?
lacto-N-tetraose + H2O
glucose + Galbeta(1-3)GlcNAcbeta(1-3)Gal
show the reaction diagram
mucin + H2O
GlcNAcbeta(1-3)Gal + GlcNAc-6Sbeta(1-3)Gal + resistant polymer
show the reaction diagram
-
desialyzed pig colonic mucin
-
?
neolacto-type glycosphingolipid + H2O
glucosylceramide + ?
show the reaction diagram
NeuAcalpha2-3Galbeta1-3GlcNAc(6-2alphaNeuAc)beta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-4Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-4Galbeta1-3GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
NeuAcalpha2-6Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
paragloboside + H2O
?
show the reaction diagram
-
-
-
-
?
polyglycosylceramides + H2O
?
show the reaction diagram
-
in absence of sodium taurodeoxycholate conversion into at least three shorter-chain glycosphingolipids, in presence of sodium taurodeoxycholate conversion into glucosylceramide
in absence of sodium taurodeoxycholate conversion into at least three shorter-chain glycosphingolipids, in presence of sodium taurodeoxycholate conversion into glucosylceramide
?
sialosylparagloboside + H2O
?
show the reaction diagram
-
-
-
-
?
sulfated glycosaminoglycan + H2O
N-acetyl-D-glucosamine-6-sulfate + ?
show the reaction diagram
-
pH 6.8
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
keratan sulfate + H2O
keratan oligosaccharides
show the reaction diagram
-
-
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Fe2+
-
1 mM, partial inhibition
Galbeta1-4GlcNAcbeta1-3Gal
-
when assayed with lacto-N-neotetraosylceramide
GlcNAcbeta1-3Gal
-
when assayed with lacto-N-neotetraosylceramide
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetate
-
slight activation
Maleate
-
slight activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.35
Keratan sulfate
-
-
3.91
lacto-N-tetraose
-
-
0.3
paragloboside
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 5.8
7.2 - 7.4
-
-
7.4
-
predigestion for 1 h, 37C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 45
-
30C: about 80% of maximal activity, 45C: about 50% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 41000, mature recombinant enzyme, SDS-PAGE; x * 43000, native enzyme, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 9
-
25C, 16 h, stable in presence of 0.2 mg of albumin per ml
208422
4.5 - 5.5
5 - 10
-
stable
208420
11
-
rapid loss of activity above
208420
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Ca2+ stabilizes
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 0.044 mg/ml protein, stable for at least 12 months
-
-20C, stable for several months
-
0C, stable for several months
-
20C, stable for at least 5 months
-
4C, stable for at least 2-3 months at a protein concentration of 0.044 mg/ml protein, 20% loss of activity in 3 h at a protein concentration of 0.003 mg/ml
-
in an ice bath, stable for 1 year
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gel filtration, ion-exchange chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
medicine
-
the enzyme reduces viscosity of alpaca semen by 48%