Information on EC 3.2.1.103 - keratan-sulfate endo-1,4-beta-galactosidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.2.1.103
-
RECOMMENDED NAME
GeneOntology No.
keratan-sulfate endo-1,4-beta-galactosidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Endohydrolysis of (1->4)-beta-D-galactosidic linkages in keratan sulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Glycosaminoglycan degradation
-
Metabolic pathways
-
SYSTEMATIC NAME
IUBMB Comments
keratan-sulfate 4-beta-D-galactanohydrolase
Hydrolyses the 1,4-beta-D-galactosyl linkages adjacent to 1,3-N-acetyl-alpha-D-glucosaminyl residues. Also acts on some non-sulfated oligosaccharides, but only acts on blood group substances when the 1,2-linked fucosyl residues have been removed (cf. EC 3.2.1.102 blood-group-substance endo-1,4-beta-galactosidase).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
endo-beta-galactosidase
-
-
-
-
endo-beta-galactosidase
-
-
galactosidase, keratosulfate endo- beta-
-
-
-
-
keratan sulfate endogalactosidase
-
-
-
-
keratanase
-
-
-
-
keratanase II
-
-
keratanase II
Bacillus sp. Ks 36
-
-
-
KSase II
-
-
KSase II
Bacillus sp. Ks 36
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
55072-01-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Ks 36
-
-
Manually annotated by BRENDA team
Bacillus sp. Ks 36
Ks 36
-
-
Manually annotated by BRENDA team
IFO-13309
-
-
Manually annotated by BRENDA team
Pseudomonas sp. IFO-13309
IFO-13309
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
A1-glycolipid + H2O
?
show the reaction diagram
-
-
-
-
?
Ab-glycolipid + H2O
?
show the reaction diagram
-
-
-
-
?
erythroglycan + H2O
?
show the reaction diagram
-
-
-
-
?
Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
-
-
-
?
Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
Fucalpha1-2Galbeta1-3GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
-
-
-
?
Gal(beta1-4)GlcNAc(beta1-3)Gal(beta1-4)sorbitol + H2O
Gal(beta1-4)GlcNAc(beta1-3)Gal + sorbitol
show the reaction diagram
-
-
-
?
Galalpha1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
?
show the reaction diagram
-
-
-
-
-
Galalpha1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
?
show the reaction diagram
-
-
-
-
?
Galalpha1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
?
show the reaction diagram
-
-
-
-
?
Galalpha1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
?
show the reaction diagram
-
no activity
-
-
-
Galbeta1-3GlcNAc(6-2alphaNeuAc)beta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
-
-
-
?
Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
Galbeta1-3[Fucalpha1-4]GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
-
-
-
?
Galbeta1-4GlcNAcbeta1-3Galbeta1-3Galbeta1-4Glc-Cer + H2O
Galbeta1-4GlcNAcbeta1-3Galbeta1-3Gal + Glc-Cer
show the reaction diagram
-
-
-
-
Galbeta1-4GlcNAcbeta1-3Galbeta1-3Galbeta1-4Glc-Cer + H2O
Galbeta1-4GlcNAcbeta1-3Galbeta1-3Gal + Glc-Cer
show the reaction diagram
-
-
-
?
Galbeta1-4GlcNAcbeta1-3Galbeta1-3Galbeta1-4Glc-Cer + H2O
Galbeta1-4GlcNAcbeta1-3Galbeta1-3Gal + Glc-Cer
show the reaction diagram
-
-
-
-
?
Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
glycosphingolipids + H2O
?
show the reaction diagram
-
lacto-N-glycosylceramide series having the common structure R-GlcAcbeta1-3Galbeta1-4Glc(or GlcNAc) are hydrolyzed at Galbeta1-4Glc (or GlcNAC) linkages. beta-Galactosyl linkages in globo series or ganglio series of glycolipids are not hydrolyzed
-
-
?
H1-glycolipid + H2O
?
show the reaction diagram
-
-
-
-
?
keratan sulfate + H2O
oligosaccharides
show the reaction diagram
-
-
-
-
?
keratan sulfate + H2O
oligosaccharides
show the reaction diagram
-
-
-
-
?
keratan sulfate + H2O
oligosaccharides
show the reaction diagram
-
-
6-O-sulfo-GlcNAcbeta1-3Gal is the major product
?
keratan sulfate + H2O
oligosaccharides
show the reaction diagram
-
-
2-acetamido-2-deoxy-6-O-sulfo-beta-D-Gal-(1-3)-D-Gal + 2-acetamido-2-deoxy-6-O-sulfo-beta-D-Glc-(1-3)-6-O-sulfoD-Gal-(1-4)-2-acetamido-2-deoxy-6-O-sulfo-beta-D-Glc-(1-3)-D-Gal
?
keratan sulfate + H2O
oligosaccharides
show the reaction diagram
-
from nasal carilage
-
-
?
keratan sulfate + H2O
oligosaccharides
show the reaction diagram
-
from bovine cornea
-
-
?
keratan sulfate + H2O
oligosaccharides
show the reaction diagram
-
from bovine cornea
more than 75% of the oligosaccharides are sulfated unbranched poly(N-acetyllactosamine) sequences, (-3/4GlcNAcbeta1-3Galbeta1-)n. SO3-beta1-3Gal accounts for almost 35% of the oligosaccharide material while 40% consists of four oligosaccharides, unbranched tetrasaccharides, hexasaccharides, octasaccharides and decasaccharides of poly(N-acetyllactosamine)type, having 3, 5, 7 and 9 sulfate residues respectively. A sulfate residue is attached to the C6 position of each N-acetylglucosamine and each internal galactose residue of the four oligosaccharides
-
keratan sulfate + H2O
oligosaccharides
show the reaction diagram
-
from bovine cornea
GlcNAcbeta1-3Gal + sulfated disaccharides, trisaccharides, tetrasaccharides, hexasaccharides, octasaccharides and decasaccharides based on the sequence (-3/4GlcNAcbeta1-3Galbeta1-), having 1, 3, 5, 7 and 9 sulfate groups
?
keratan sulfate + H2O
oligosaccharides
show the reaction diagram
-
from teleost skin
no production of GlcNAcbeta1-3Gal, 6-O-sulfo-GlcNAcbeta1-3Gal is one of the major products
?
keratan sulfate + H2O
oligosaccharides
show the reaction diagram
-
from teleost skin
6-O-sulfo-GlcNAcbeta1-3Gal is one of the major products
?
keratan sulfate + H2O
oligosaccharides
show the reaction diagram
-
from teleost skin
GlcNAcbeta1-3Gal
?
keratan sulfate + H2O
oligosaccharides
show the reaction diagram
-
keratan sulfate chains from bovine articular cartilage. Presence of fucose on N-acetylglucosamine residues in keratan sulfates protects both of the adjacent unsulfated galactose residues from keratanase cleavage
GlcNAc(6S)beta1-3Galbeta1-4(Fucalpha1-3)GlcNAc(6S)beta1-3Galbeta1-4GlcNAc(6S)beta1-3Gal-ol + GlcNAc(6S)beta1-3Galbeta1-4(Fucalpha-3)GlcNAc(6S)beta1-3Galbeta1-4GlcNAc(6S)beta1-6(Galbeta1-33)GalNAc-ol
?
keratan sulfate + H2O
oligosaccharides
show the reaction diagram
Pseudomonas sp., Pseudomonas sp. IFO-13309
-
corneal keratan sulfate. Compared with corneal keratan sulfate, keratan sulfates from human nucleus pulposus and shark cartilage are attacked at lower rates with a resultant production of oligosaccharides of relative large size
differing in size and sulfate content
?
keratan sulfate + H2O
oligosaccharide
show the reaction diagram
-
-
-
-
?
keratan sulfate + H2O
oligosaccharide
show the reaction diagram
Bacillus sp., Bacillus sp. Ks 36
-
catalysis mechanism of KSase II involves a sugar oxazolinium ion that requires the 6-sulfate group in the GlcNAc residue not only in hydrolysis of KS chains, but also in oligomerization of oxazoline monomers
-
-
?
keratan sulfate + H2O
keratan oligosaccharides
show the reaction diagram
-
keratanase II, an endo-beta-N-acetylglucosaminidase, cleaves the beta(1-3)-glycosidic bond of a 6-O-sulphated GlcNAc in keratin sulfate
-
-
-
lacto-N-fucopentaose I + H2O
?
show the reaction diagram
-
-
-
-
?
lacto-N-fucopentaose II + H2O
?
show the reaction diagram
-
-
-
-
?
lacto-N-fucopentaose III + H2O
?
show the reaction diagram
-
-
-
-
?
lacto-N-neotetraosylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
lacto-N-tetraitol + H2O
sorbitol + Galbeta(1-3)GlcNAcbeta(1-3)Gal
show the reaction diagram
-
-
-
?
lacto-N-tetraose + H2O
glucose + Galbeta(1-3)GlcNAcbeta(1-3)Gal
show the reaction diagram
-
-
-
?
lacto-N-tetraose + H2O
glucose + Galbeta(1-3)GlcNAcbeta(1-3)Gal
show the reaction diagram
-
-
-
-
?
mucin + H2O
GlcNAcbeta(1-3)Gal + GlcNAc-6Sbeta(1-3)Gal + resistant polymer
show the reaction diagram
-
desialyzed pig colonic mucin
-
?
neolacto-type glycosphingolipid + H2O
glucosylceramide + ?
show the reaction diagram
-
hydrolysis of the internal beta1,4-galactosyl linkage of various neolacto-type glycosphingolipids to produce glucosylceramides
-
?
NeuAcalpha2-3Galbeta1-3GlcNAc(6-2alphaNeuAc)beta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
-
-
-
?
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
-
-
-
?
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
-
-
-
-
?
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
-
-
-
?
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-4Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-4Galbeta1-3GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
-
-
-
?
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-4Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc-Cer + H2O
NeuAcalpha2-3Galbeta1-4GlcNAcbeta1-4Galbeta1-3GlcNAcbeta1-3Gal + Glc-Cer
show the reaction diagram
-
no activity
-
-
-
NeuAcalpha2-6Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc + H2O
?
show the reaction diagram
-
-
-
-
?
paragloboside + H2O
?
show the reaction diagram
-
-
-
-
?
polyglycosylceramides + H2O
?
show the reaction diagram
-
in absence of sodium taurodeoxycholate conversion into at least three shorter-chain glycosphingolipids, in presence of sodium taurodeoxycholate conversion into glucosylceramide
in absence of sodium taurodeoxycholate conversion into at least three shorter-chain glycosphingolipids, in presence of sodium taurodeoxycholate conversion into glucosylceramide
?
sialosylparagloboside + H2O
?
show the reaction diagram
-
-
-
-
?
sulfated glycosaminoglycan + H2O
N-acetyl-D-glucosamine-6-sulfate + ?
show the reaction diagram
-
pH 6.8
-
-
?
lacto-N-tetraose + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no hydrolysis of phenyl beta-D-galactoside
-
-
-
additional information
?
-
-
sialosyl substitution at the terminal galactosyl residue of lacto-N-neotetraosyl structure of paragloboside greatly enhances the hydrolyzability of the internal Galbeta1-4Glc linkage, thus producing a high yield of sialotetrasaccharide (AcNeualpha2-3Galbeta1-4GlcNAcbeta-3Gal). The Galbeta1-4GlcNAc linkage located in the middle of the repeating Galbeta1-4GlcNAc(or Glc) unit seen in H2-glycolipid or Ab-glycolipid, (R-Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer) is preferentially hydrolyzed relative to the Galbeta1-4Glc linkage directly attached to ceramide. The branched structure as is found in H3-glycolipid greatly reduces the hydrolyzability
-
-
-
additional information
?
-
-
hydrolyzes glycoconjugates containing N-acetyllactosamine repeating units
-
-
-
additional information
?
-
-
hydrolyzes the type 2 sequence Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc at about twice the rate of the type 1 isomer, Galbeta1-3GlcNAcbeta1-3Galbeta1-4Glc
-
-
-
additional information
?
-
-
AB active blood group substance can be hydrolyzed only after Smith degradation
-
-
-
additional information
?
-
-
no activity with Galbeta1-4GlcNAcbeta1-6Galbeta1-4GlcNAcbeta1-6(Galbeta1-3)GalNAc-ol
-
-
-
additional information
?
-
-
induced with high efficiency in culture medium containing Smith-degraded gastric mucin
-
-
?
additional information
?
-
-
the enzyme is produced without induction by keratan sulfate
-
-
?
additional information
?
-
Pseudomonas sp. IFO-13309
-
no hydrolysis of phenyl beta-D-galactoside
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
induced with high efficiency in culture medium containing Smith-degraded gastric mucin
-
-
?
additional information
?
-
-
the enzyme is produced without induction by keratan sulfate
-
-
?
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Ag+
-
1 mM, complete inhibition
Cu2+
-
1 mM, 30% inhibition
Cu2+
-
1 mM, partial inhibition
Fe2+
-
1 mM, partial inhibition
-
Galbeta1-4GlcNAcbeta1-3Gal
-
when assayed with lacto-N-neotetraosylceramide
GlcNAcbeta1-3Gal
-
when assayed with lacto-N-neotetraosylceramide
Hg2+
-
1 mM, complete inhibition
PCMB
-
0.25 mM, complete inhibition
PCMB
-
1 mM, complete inhibition
Zn2+
-
1 mM, 30% inhibition
Zn2+
-
1 mM, partial inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
acetate
-
slight activation
Maleate
-
slight activation
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.35
-
Keratan sulfate
-
-
3.91
-
lacto-N-tetraose
-
-
0.3
-
paragloboside
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
-
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
5.8
-
hydrolysis of keratan sulfate from nasal cartilage or Galalpha1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glc-Cer
6
-
-
hydrolysis of keratan sulfate
6
-
-
37C, 4 h
7.2
7.4
-
-
7.4
-
-
predigestion for 1 h, 37C
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
predigestion for 1 h, pH 7.4
37
-
-
pH 6.0, 4 h
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
45
-
30C: about 80% of maximal activity, 45C: about 50% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
28000
-
-
gel filtration
30000
-
-
gel filtration
30000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 41000, mature recombinant enzyme, SDS-PAGE; x * 43000, native enzyme, SDS-PAGE
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.5
9
-
25C, 16 h, stable in presence of 0.2 mg of albumin per ml
4
-
-
17 h at room temperature, rapid loss of activity below
4
-
-
rapid loss of activity below
4
-
-
17 h at room temperature, rapid loss of activity below
4.5
5.5
-
17 h at room temperature, retains 80 to 100% activity
6
-
-
17 h at room temperature, rapid loss of activity above
11
-
-
rapid loss of activity above
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
1 h, 50% loss of activity, bovine serum albumin, 0.4 mg/ml, prevents inactivation
37
-
-
0.003 mg/ml protein, 40% loss of activity after 30 min
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ca2+ stabilizes
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, 0.044 mg/ml protein, stable for at least 12 months
-
4C, stable for at least 2-3 months at a protein concentration of 0.044 mg/ml protein, 20% loss of activity in 3 h at a protein concentration of 0.003 mg/ml
-
-20C, stable for several months
-
0C, stable for several months
-
in an ice bath, stable for 1 year
-
20C, stable for at least 5 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
gel filtration, ion-exchange chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
analysis
-
establishment of a highly sensitive liquid chromatography tandem mass spectrometry method to analyze disaccharides produced from keratan sulfate by keratanase II
analysis
-
the enzyme can be used to distinguish between GlcNAcbeta1-6Gal and GlcNAcbeta1-3Gal units within linear backbone sequences of all known types of oligo-(N-acetyllactosamino)glycans
analysis
-
useful for studying the structure of glycoproteins and oligosaccharides
analysis
-
enzyme can be useful in studying the precise structures of keratan sulfates, related glycoproteins and oligosaccharides
analysis
-
the enzyme can be used for studies on structural variability of keratan sulfates of different origin
analysis
Pseudomonas sp. IFO-13309
-
the enzyme can be used for studies on structural variability of keratan sulfates of different origin
-