Cloned (Comment) | Organism |
---|---|
high level expression of thermostable Bacillus licheniformis alpha-amylase in Escherichia coli strain BL21-CodonPlus(DE3)-RIL mainly in inclusion bodies, subcloning in Escherichia coli strain DH5alpha | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | P06278 | - |
- |
Bacillus licheniformis ATCC 27811 | P06278 | - |
- |
Purification (Comment) | Organism |
---|---|
solubilized recombinant enzyme 138fold from Escherichia coli by cation exchange chromatography | Bacillus licheniformis |
Renatured (Comment) | Organism |
---|---|
solubilization of recombinant enzyme from inclusion bodies after expression in Escherichia coli without the use of denaturing agents, extraction by resuspending in 50 mmol/l sodium acetate, pH 5.0, containing 20% v/v glycerol followed by stirring at 40°C for 3-4 h, and subsequent centrifugation | Bacillus licheniformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1343 | - |
purified recombinant enzyme, pH 5.0, 60°C | Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Bacillus licheniformis |