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Literature summary for 3.1.99.B1 extracted from

  • Matsui, E.; Kawasaki, S.; Ishida, H.; Ishikawa, K.; Kosugi, Y.; Kikuchi, H.; Kawarabayashi, Y.; Matsui, I.
    Thermostable flap endonuclease from the archaeon, Pyrococcus horikoshii, cleaves the replication fork-like structure endo/exonucleolytically (1999), J. Biol. Chem., 274, 18297-18309.
    View publication on PubMed
Search for more literature for 3.1.99.B1

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Pyrococcus horikoshii

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM CaCl2 results in greater than 90% inhibition Pyrococcus horikoshii
EDTA 5 mM, complete inhibition Pyrococcus horikoshii
KCl 60 mM, the activity increases 1.3-fold, 100 mM KCl result in significant inhibition of activity Pyrococcus horikoshii
Mg2+ addition of 1 mM MgCl2 increases the activity 1.4-fold. 10 mM MgCl2 results in greater than 90% inhibition Pyrococcus horikoshii
Mn2+ addition of 0.1 mM MgCl2 increases the activity 1.5-fold. 10 mM MnCl2 results in greater than 90% inhibition Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 60 mM, the activity increases 1.3-fold, 100 mM KCl result in significant inhibition of activity Pyrococcus horikoshii
Mg2+ addition of 1 mM MgCl2 increases the activity 1.4-fold. 10 mM MgCl2 results in greater than 90% inhibition Pyrococcus horikoshii
Mn2+ addition of 0.1 mM MgCl2 increases the activity 1.5-fold. 10 mM MnCl2 results in greater than 90% inhibition Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 gel filtration Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O50123
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii

Storage Stability

Storage Stability Organism
4°C or 20°C, stable for over 3 months without loss of activity Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme cleaves replication fork-like substrates and 5' double-strand flap structures using both flap endonuclease and 5'->3'-exonuclease activities Pyrococcus horikoshii ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 the optimal temperature could not be determined since the activity peak could not be detected Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 thermostability is dependent on the concentration of NaCl. Addition of 1 M NaCl increases the thermostability Pyrococcus horikoshii
95
-
 at a final concentration of 0.3 mg/ml, the half-life is 2 h in the presence of 1 M NaCl and 20 min in 50 mM NaCl Pyrococcus horikoshii
102
-
 Tm-value is 102°C in 0.0085 and 0.0014 mM protein with 1 M NaCl concentrations. Values of Tm are not dependent on the protein concentration Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Pyrococcus horikoshii

pH Range

pH Minimum pH Maximum Comment Organism
6 7 the activity of phFEN decreases immediately at pH values below 6.0 and over 7.0. The activity can not be detected at pH 5.0 and decreased to 24% at pH 9.4 Pyrococcus horikoshii

pI Value

Organism Comment pI Value Maximum pI Value
Pyrococcus horikoshii the pI value of the phFEN is greater than 9.3
-
 additional information