Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition | Brassica oleracea | |
additional information | no inhibiton by EDTA | Brassica oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Brassica oleracea | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | absolutely dependent on, no activity without Ca2+ | Brassica oleracea | |
Ca2+ | the enzyme is activated by Ca2+, in absence of Ca2+ it exhibits 70% of maximal activity | Brassica oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | Brassica oleracea | - |
choline + phosphatidate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Brassica oleracea | - |
- |
- |
Capsella bursa-pastoris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme partially by preparation of the 13000 rpm fraction | Arabidopsis thaliana |
native enzyme partially by preparation of the 13000 rpm fraction | Capsella bursa-pastoris |
native enzyme partially by preparation of the membrane fraction, solubilization with Triton X-100, ammonium sulfate fractionation, and diethylaminoethyl-cellulose chromatography | Brassica oleracea |
native enzyme partially from cabbage leaves by acetone precipitation | Brassica oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
leaf | - |
Brassica oleracea | - |
leaf | - |
Capsella bursa-pastoris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0005 | - |
partially purified enzyme, pH 7.4, 30°C | Capsella bursa-pastoris |
0.0011 | - |
partially purified enzyme, pH 7.4, 30°C | Arabidopsis thaliana |
0.03 | 0.04 | partially purifed enzyme, pH 7.4, 30°C | Brassica oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycosylinositol phosphoceramide + H2O | - |
Arabidopsis thaliana | phytoceramide-1-phosphate + glycosylinositol | phytoceramide-1-phosphate with an alpha-hydroxy fatty acid | ? | |
glycosylinositol phosphoceramide + H2O | - |
Brassica oleracea | phytoceramide-1-phosphate + glycosylinositol | phytoceramide-1-phosphate with an alpha-hydroxy fatty acid | ? | |
glycosylinositol phosphoceramide + H2O | - |
Capsella bursa-pastoris | phytoceramide-1-phosphate + glycosylinositol | phytoceramide-1-phosphate with an alpha-hydroxy fatty acid | ? | |
additional information | the enzyme does not hydrolyze glycosylinositol phosphoceramide | Brassica oleracea | ? | - |
? | |
additional information | the enzyme hydrolyzes glycosylinositol phosphoceramide specifically, but not glycerophospholipids, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, or sphingomyelin, MALDI-TOF mass spectrometry product identification, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | the enzyme hydrolyzes glycosylinositol phosphoceramide specifically, but not glycerophospholipids, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, or sphingomyelin, MALDI-TOF mass spectrometry product identification, overview | Brassica oleracea | ? | - |
? | |
additional information | the enzyme hydrolyzes glycosylinositol phosphoceramide specifically, but not glycerophospholipids, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, or sphingomyelin, MALDI-TOF mass spectrometry product identification, overview | Capsella bursa-pastoris | ? | - |
? | |
phosphatidylcholine + H2O | - |
Brassica oleracea | choline + phosphatidate | - |
? | |
phosphatidylcholine + H2O | from egg yolk | Brassica oleracea | choline + phosphatidate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GIPC-PLD | - |
Arabidopsis thaliana |
GIPC-PLD | - |
Brassica oleracea |
GIPC-PLD | - |
Capsella bursa-pastoris |
PC-PLD | - |
Brassica oleracea |
PLD | - |
Arabidopsis thaliana |
PLD | - |
Brassica oleracea |
PLD | - |
Capsella bursa-pastoris |
sphingolipid-specific phospholipase D | - |
Arabidopsis thaliana |
sphingolipid-specific phospholipase D | - |
Brassica oleracea |
sphingolipid-specific phospholipase D | - |
Capsella bursa-pastoris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
30 | - |
assay at | Brassica oleracea |
30 | - |
assay at | Capsella bursa-pastoris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Brassica oleracea |
7.4 | - |
assay at | Arabidopsis thaliana |
7.4 | - |
assay at | Capsella bursa-pastoris |
7.5 | - |
- |
Brassica oleracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | activity range | Brassica oleracea |
4.5 | 7 | activity range | Brassica oleracea |