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Literature summary for 3.1.4.4 extracted from

  • Chae, Y.C.; Kim, J.H.; Kim, K.L.; Kim, H.W.; Lee, H.Y.; Heo, W.D.; Meyer, T.; Suh, P.G.; Ryu, S.H.
    Phospholipase D activity regulates integrin-mediated cell spreading and migration by inducing GTP-Rac translocation to the plasma membrane (2008), Mol. Biol. Cell, 19, 3111-3123.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.4

Activating Compound

Activating Compound Comment Organism Structure
fibronectin in OVAR-3 cells fibronectin-mediated integrin activation specifically induces cell spreading which activates PLD Homo sapiens

Application

Application Comment Organism
molecular biology PLD product phosphatidic acid acts as a membrane anchor of Rac1. The C-terminal polybasic motif of Rac1 is responsible for direct interaction with phosphatidic acid. It is shown that phosphatidic acid induces dissociation of Rho-guanine nucleotide dissociation inhibitor from Rac1 and that phosphatidic acid-mediated Rac1 localization is important for integrin-mediated lamellipodia formation, cell spreading, and migration Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information by PLD RNAi experiments it is shown that PLD activity is required for integrin-mediated cell spreading. RNAi targeting PLD1 or PLD2, or both isoforms reduces integrin-mediated phosphatidic acid. In correlation with phosphatidic acid generation, PLD knockdown reduces cell spreading which is restored by direct phosphatidic acid treatment Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibition of the PLD product formation phosphatidic acid by adding 1-butanol significantly decreases cell spreading; inhibition of the PLD product formation phosphatidic acid by adding 1-butanol significantly decreases cell spreading Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol in resting OVCAR-3 cells PLD1 is predominatly localised to vesicular structure in the cytosol. During integrin-mediated spreading cells, both PLD1 and PLD2 predominantly localize at the plasma membrane and distribution of PLD1 closely resembles that of PLD2 Homo sapiens 5829
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plasma membrane
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 Homo sapiens 5886
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens PLD product phosphatidic acid acts as a membrane anchor of Rac1. The C-terminal polybasic motif of Rac1 is responsible for direct interaction with phosphatidic acid. It is shown that phosphatidic acid induces dissociation of Rho-guanine nucleotide dissociation inhibitor from Rac1 and that phosphatidic acid-mediated Rac1 localization is important for integrin-mediated lamellipodia formation, cell spreading, and migration ?
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 ?
additional information Homo sapiens PLD product phosphatidic acid acts as a membrane anchor of Rac1. The C-terminal polybasic motif of Rac1 is responsible for direct interaction with phosphatidic acid. Phosphatidic acid induces dissociation of Rho-guanine nucleotide dissociation inhibitor from Rac1 and that phosphatidic acid-mediated Rac1 localization is important for integrin-mediated lamellipodia formation, cell spreading, and migration ?
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O14939
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Homo sapiens Q13393
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Source Tissue

Source Tissue Comment Organism Textmining
OVCAR-3 cell
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 Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PLD product phosphatidic acid acts as a membrane anchor of Rac1. The C-terminal polybasic motif of Rac1 is responsible for direct interaction with phosphatidic acid. It is shown that phosphatidic acid induces dissociation of Rho-guanine nucleotide dissociation inhibitor from Rac1 and that phosphatidic acid-mediated Rac1 localization is important for integrin-mediated lamellipodia formation, cell spreading, and migration Homo sapiens ?
-
 ?
additional information PLD product phosphatidic acid acts as a membrane anchor of Rac1. The C-terminal polybasic motif of Rac1 is responsible for direct interaction with phosphatidic acid. Phosphatidic acid induces dissociation of Rho-guanine nucleotide dissociation inhibitor from Rac1 and that phosphatidic acid-mediated Rac1 localization is important for integrin-mediated lamellipodia formation, cell spreading, and migration Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
phospholipase D
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 Homo sapiens
PLD1
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 Homo sapiens
PLD2
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 Homo sapiens