Activating Compound | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | required for activity with membrane-bound phosphatidylinositol, slight stimulation with isolated phosphatidylinositol | Rattus norvegicus | |
oleic acid | causes a marked stimulation of the enzyme activity when the membrane-bound substrate is used | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates the activity and can not be replaced by MnCl2, MgCl2 or ZnCl2 | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
female Sprague-Dawley rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | prepared from rat slow (soleus) and fast (extensor digitorum longus) muscles | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme specific activity is higher in cytosol prepared from soleus muscle than in that from extensor digitorum longus muscle | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylinositol + H2O | enzyme hydrolyses both membrane-bound and extracted phosphatidylinositol | Rattus norvegicus | 1D-myo-inositol 1-phosphate + diacylglycerol | in vitro myo-inositol 1,2-cyclic phosphate, myo-inositol 1-phosphate, and glycerophosphoinositol in the molar proportions approx. 2:1:1 | ? |
Synonyms | Comment | Organism |
---|---|---|
phosphatidylinositol phosphodiesterase | - |
Rattus norvegicus |
PtdIns phosphodiesterase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
two pH optima are apparent, at pH 5.5 and pH 7.4 | Rattus norvegicus |
7.4 | - |
two pH optima are apparent, at pH 5.5 and pH 7.4 | Rattus norvegicus |