Urea |
reversible inactivation. At low concentrations of urea, the first detected alteration in properties is an increase in the activity of the enzyme. This is followed by inactivation and the release of half of the zinc content when the amount of urea reaches levels of 2 M. Intrinsic tryptophan fluorescence and circular dichroism ellipticity change in the range 2.5 to 8 M urea, signalling dimer dissociation, followed by one major monomer unfolding transition at 6-8 M urea as indicated by ANS fluorescence and KI fluorescence quenching |
Gadus morhua |
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