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Literature summary for 3.1.21.B1 extracted from

  • Shiraishi, M.; Ishino, S.; Heffernan, M.; Cann, I.; Ishino, Y.
    The mesophilic archaeon Methanosarcina acetivorans counteracts uracil in DNA with multiple enzymes EndoQ, ExoIII, and UDG (2018), Sci. Rep., 8, 15791 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.B1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanosarcina acetivorans

Protein Variants

Protein Variants Comment Organism
D192A inactive mutant enzyme EndoQ Methanosarcina acetivorans

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans Q8TT02
-
-
Methanosarcina acetivorans ATCC 35395 Q8TT02
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina acetivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O the enzyme recognizes and cleaves the DNA containing dU, deoxyinosine (dI), and apurinic/apyrimidinic site in DNA generating 23-, 24-, and 24-nucleotide fragments, respectively. EndoQ incises the DNA backbone at the 5'-sides of the damaged sites Methanosarcina acetivorans ?
-
 ?
DNA + H2O the enzyme recognizes and cleaves the DNA containing dU, deoxyinosine (dI), and apurinic/apyrimidinic site in DNA generating 23-, 24-, and 24-nucleotide fragments, respectively. EndoQ incises the DNA backbone at the 5'-sides of the damaged sites Methanosarcina acetivorans ATCC 35395 ?
-
 ?

Synonyms

Synonyms Comment Organism
EndoQ
-
 Methanosarcina acetivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Methanosarcina acetivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Methanosarcina acetivorans

General Information

General Information Comment Organism
physiological function the enzyme appears to be involved in the repair of dU, dI, and apurinic/apyrimidinic sites Methanosarcina acetivorans