Information on EC 3.1.21.B1 - endonuclease Q

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.21.B1
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
endonuclease Q
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endonucleolytic cleavage of the phosphodiester bond 5' from deoxyinosine, forming a 5'-phosphate terminus and a 3'-hydroxyl terminus. DNAs containing deoxyuridine, 7-deaza-2'-deoxyxanthosine or an apurinic/apyrimidinic site are also cleaved
show the reaction diagram
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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the enzyme is involved in damaged DNA repair. EndoQ predominately functions over EndoV (EC 3.1.21.7) to start the process of damaged base repair in Pyrococcus furiosus cells
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-deaza-2'-deoxyxanthosine-containing DNA + H2O
?
show the reaction diagram
apurinic/apyrimidinic site-containing DNA + H2O
?
show the reaction diagram
deoxyinosine-containing deoxyoligonucleotide + H2O
?
show the reaction diagram
deoxyinosine-containing DNA + H2O
?
show the reaction diagram
deoxyuridine-containing DNA + H2O
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
deoxyinosine-containing deoxyoligonucleotide + H2O
?
show the reaction diagram
-
the enzyme is involved in damaged DNA repair
-
-
?
deoxyinosine-containing DNA + H2O
?
show the reaction diagram
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 9.5
-
pH 8.5: about 60% of maximal activity, pH 9.5: optimum, pH 10.0: less than 19% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70 - 80
-
70°C: about 50% of maximal activity, 80°C: about 35% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.1
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calculated from sequence
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47653
-
x * 47653, calculated from sequence
48080
x * 48080, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
overexpression in Escherichia coli
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