Any feedback?
Literature summary for 3.1.21.B1 extracted from
- A novel endonuclease that may be responsible for damaged DNA base repair in Pyrococcus furiosus (2015), Nucleic Acids Res., 43, 2853-2863.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyrococcus furiosus |
| expression in Escherichia coli | Thermococcus kodakarensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47653 | - |
x * 47653, calculated from sequence | Pyrococcus furiosus |
| 48080 | - |
x * 48080, calculated from sequence | Thermococcus kodakarensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxyinosine-containing DNA + H2O | Pyrococcus furiosus | the enzyme cleaves the phosphodiester bond 5' from deoxyinosine. The enzyme is an endonuclease that functions in a repair process for damaged bases in archaeal cells | ? | - |
? |  |
| deoxyinosine-containing DNA + H2O | Thermococcus kodakarensis | the enzyme cleaves the phosphodiester bond 5' from deoxyinosine. The enzyme is an endonuclease that functions in a repair process for damaged bases in archaeal cells | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q8U0N5 | - |
- |
| Thermococcus kodakarensis | Q5JI33 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
- |
Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-deaza-2'-deoxyxanthosine-containing DNA + H2O | normal ssDNA, dsDNA and the DNA containing a G/T mismatch are completely resistant to the cleavage reaction. The lengths of the products indicate that PfuEndoQ cleaves DNA on the 5'-side of the lesions | Pyrococcus furiosus | ? | - |
? | |
| 7-deaza-2'-deoxyxanthosine-containing DNA + H2O | the enzyme cleaves a ligatable nick (5'-phosphate and 3'-hydroxyl termini) on substrates after the cleavage | Thermococcus kodakarensis | ? | - |
? | |
| apurinic/apyrimidinic site-containing DNA + H2O | normal ssDNA, dsDNA and the DNA containing a G/T mismatch are completely resistant to the cleavage reaction. The lengths of the products indicate that PfuEndoQ cleaves DNA on the 5'-side of the lesions | Pyrococcus furiosus | ? | - |
? | |
| apurinic/apyrimidinic site-containing DNA + H2O | the enzyme cleaves a ligatable nick (5'-phosphate and 3'-hydroxyl termini) on substrates after the cleavage | Thermococcus kodakarensis | ? | - |
? | |
| deoxyinosine-containing DNA + H2O | the enzyme cleaves the phosphodiester bond 5' from deoxyinosine. The enzyme is an endonuclease that functions in a repair process for damaged bases in archaeal cells | Pyrococcus furiosus | ? | - |
? | |
| deoxyinosine-containing DNA + H2O | the enzyme cleaves the phosphodiester bond 5' from deoxyinosine. The enzyme is an endonuclease that functions in a repair process for damaged bases in archaeal cells | Thermococcus kodakarensis | ? | - |
? | |
| deoxyinosine-containing DNA + H2O | the enzyme cleaves the phosphodiester bond 5' from deoxyinosine | Thermococcus kodakarensis | ? | - |
? | |
| deoxyinosine-containing DNA + H2O | the enzyme cleaves the phosphodiester bond 5' from deoxyinosine. The normal ssDNA, dsDNA and the DNA containing a G/T mismatch are completely resistant to the cleavage reaction. The lengths of the products indicate that PfuEndoQ cleaves DNA on the 5'-side of the lesions | Pyrococcus furiosus | ? | - |
? | |
| deoxyuridine-containing DNA + H2O | normal ssDNA, dsDNA and the DNA containing a G/T mismatch are completely resistant to the cleavage reaction. The lengths of the products indicate that PfuEndoQ cleaves DNA on the 5'-side of the lesions | Pyrococcus furiosus | ? | - |
? | |
| deoxyuridine-containing DNA + H2O | the enzyme cleaves a ligatable nick (5'-phosphate and 3'-hydroxyl termini) on substrates after the cleavage | Thermococcus kodakarensis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48080, calculated from sequence | Thermococcus kodakarensis |
| ? | x * 47653, calculated from sequence | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EndoQ | - |
Pyrococcus furiosus |
| EndoQ | - |
Thermococcus kodakarensis |
| PF1551 | locus name | Pyrococcus furiosus |
| TK0887 | locus name | Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
assay at | Pyrococcus furiosus |
| 75 | - |
assay at | Thermococcus kodakarensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pyrococcus furiosus |
| 8 | - |
assay at | Thermococcus kodakarensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
