Cloned (Comment) | Organism |
---|---|
gene tes B, DNA sequence determination and analysis, no sequence similarity between Escherichia coli thioesterase II and the two types of mammalian thioesterases, i.e. the chain-terminating enzymes of de novo fatty acid synthesis | Escherichia coli |
gene tesB, overexpression in the Escherichia coli host strain | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop method, protein solution: 10 mg/ml, 5 mM NH4OH, 2 mM DTT, over equal volume of reservoir solution: 1-2 M NaCl, 0.1 M sodium formate, pH 6.0, 2 mM N,N-dimethyl-dodecylamine oxide-LDAO, few days, X-ray structure determination and analysis | Escherichia coli |
General Stability | Organism |
---|---|
bovine serum albumin stabilizes | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldicarbonate | hydroxylamine reverses | Escherichia coli | |
IAA | - |
Escherichia coli | |
additional information | no inhibition by iodoacetate | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Escherichia coli | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
123000 | - |
recombinant enzyme, native PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AGG2 | gene tesB | - |
Purification (Comment) | Organism |
---|---|
native wild-type: 8000fold to homogeneity | Escherichia coli |
recombinant wild-type: 4fold to homogeneity | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
93.8 | - |
purified recombinant enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + H2O | - |
Escherichia coli | CoA + a carboxylate | - |
? | |
arachidoyl-CoA + H2O | - |
Escherichia coli | CoA + arachidate | - |
? | |
decanoyl-CoA + H2O | - |
Escherichia coli | CoA + decanoate | - |
? | |
additional information | decanoyl pantetheine is no substrate | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 31842-32100, recombinant enzyme, electrospray ionization mass spectroscopy and SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |