Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sodium deoxycholate | or sodium taurodeoxycholate, required, optimum concentration 6 mM | Dasyatis pastinaca | |
sodium taurodeoxycholate | or sodium deoxycholate, required, optimum concentration 4 mM | Dasyatis pastinaca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10 mM, complete inhibition | Dasyatis pastinaca | |
EGTA | 10 mM, complete inhibition | Dasyatis pastinaca |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | absolutely required, optimum concentration 8 mM | Dasyatis pastinaca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
PAGE | Dasyatis pastinaca |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 50%, 97% of initial activity | Dasyatis pastinaca |
Acetone | 50%, no changes in activity | Dasyatis pastinaca |
acetonitrile | 50%, 15% increase in activity | Dasyatis pastinaca |
Ethanol | 50%, 23% increase in activity | Dasyatis pastinaca |
Methanol | 50%, no changes in activity | Dasyatis pastinaca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dasyatis pastinaca | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | small amount of N-glycosylation | Dasyatis pastinaca |
Purification (Comment) | Organism |
---|---|
- |
Dasyatis pastinaca |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | - |
Dasyatis pastinaca | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
290 | - |
pH 9.5, 45°C, presence of sodium deoxycholate and CaCl2 | Dasyatis pastinaca |
Storage Stability | Organism |
---|---|
-20°C, 120 days, 95% residual activity | Dasyatis pastinaca |
4°C, 120 days, 85% residual activity | Dasyatis pastinaca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-stearoyl-2-oleoyl-sn-glycerol-3-phosphocholine + H2O | - |
Dasyatis pastinaca | 1-stearoyl-sn-glycero-3-phosphocholine + oleic acid | hydrolysis occurs only in sn2 position of phospholipids | ? | |
egg yolk + H2O | - |
Dasyatis pastinaca | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 14000, SDS-PAGE | Dasyatis pastinaca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Dasyatis pastinaca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
20 min, 80% residual activity | Dasyatis pastinaca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Dasyatis pastinaca |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
maintains more than 70% of activity after incubation at pH 2 | Dasyatis pastinaca |