Application | Comment | Organism |
---|---|---|
medicine | the purified enzyme shows strong bactericidal activity against Burkholderia pseudomallei (minimum inhibitory concentration above 0.015 mg/ml) and Enterobacter aerogenes (minimum inhibitory concentration above 0.03 mg/ml) | Echis carinatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13862 | - |
x * 13862, MALDI-TOF mass spectrometry | Echis carinatus |
15000 | - |
x * 15000, SDS-PAGE | Echis carinatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Echis carinatus | - |
saw-scaled viper | - |
Purification (Comment) | Organism |
---|---|
C8 Sepharose column chromatography, C18 Sepharose column chromatography, and Superdex G-75 gel filtration | Echis carinatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Echis carinatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1034 | - |
crude venom, pH and temperature not specified in the publication | Echis carinatus |
2312 | - |
purified enzyme, pH and temperature not specified in the publication | Echis carinatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | - |
Echis carinatus | 1-acylglycerophosphocholine + fatty acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 15000, SDS-PAGE | Echis carinatus |
? | x * 13862, MALDI-TOF mass spectrometry | Echis carinatus |
Synonyms | Comment | Organism |
---|---|---|
PLA2 | - |
Echis carinatus |
toxin I | - |
Echis carinatus |
Tx-1 | - |
Echis carinatus |
General Information | Comment | Organism |
---|---|---|
physiological function | the phospholipase A2 toxin I displays a heparin binding C-terminal region, which is probably responsible for the cytotoxic and bactericidal effects. Toxin I is able to induce cytotoxicity on THP-1 cells in vitro as well as lethality in BALB/c mice. Toxin II also induces mild myotoxic effects on mouse skin, but is devoid of hemolytic effects on human erythrocytes up to 0.5 mg/ml | Echis carinatus |