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Literature summary for 2.8.2.5 extracted from

  • Mizumoto, S.; Mikami, T.; Yasunaga, D.; Kobayashi, N.; Yamauchi, H.; Miyake, A.; Itoh, N.; Kitagawa, H.; Sugahara, K.
    Chondroitin 4-O-sulfotransferase-1 is required for somitic muscle development and motor axon guidance in zebrafish (2009), Biochem. J., 419, 387-399.
    View publication on PubMed
Search for more literature for 2.8.2.5

Cloned(Commentary)

Cloned (Comment) Organism
gene C4ST-1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis Danio rerio
gene C4ST-2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis Danio rerio

Protein Variants

Protein Variants Comment Organism
additional information functional knockdown of C4ST-1 with specific antisense morpholino-oligonucleotides leads to a marked decrease in the 4-O-sulfation and amount of chondroitin sulfate in the embryos. Consistent with the preferential expression in the rostrocaudal axis, C4ST-1 morphants display morphological defects exemplified by a ventrally bent trunk and a curled and/or kinky tail, largely due to misregulated myotomal myod expression, implying perturbation of axial muscle differentiation in somites. Rescue of morphological phenotypes of the MO1-injected embryos by injection with zebrafish C4ST-1 RNA, phenotypes, overview Danio rerio

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + chondroitin Danio rerio
-
 adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Danio rerio Q75W66
-
-
Danio rerio Q7T3S3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Danio rerio
-
embryo
-
 Danio rerio
-
additional information C4ST-1 expression pattern, overview Danio rerio
-
muscle
-
 Danio rerio
-
notochord
-
 Danio rerio
-
rhombencephalon
-
 Danio rerio
-
somite
-
 Danio rerio
-
telencephalon
-
 Danio rerio
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 recombinant enzyme Danio rerio
additional information
-
 recombinant enzyme activity with chondroitin and partially desulfated dermatan sulfate Danio rerio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + chondroitin
-
 Danio rerio adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
 ?
3'-phosphoadenylyl sulfate + chondroitin recombinant C4ST-1 shows a substrate preference for chondroitin and catalysed the 4-O-sulfation of GalNAc residues Danio rerio adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
-
 ?
additional information C4ST-1 also shows activity with partially desulfated dermatan sulfate, strong preference of zebrafish C4ST-1 for the sequence -GlcA-GalNAc(4S)-GlcA- over -IdoA-GalNAc(4S)-IdoA- Danio rerio ?
-
 ?
additional information C4ST-2 also shows activity with partially desulfated dermatan sulfate Danio rerio ?
-
 ?

Synonyms

Synonyms Comment Organism
C4ST-1
-
 Danio rerio
C4ST-2
-
 Danio rerio
chondroitin 4-O-sulfotransferase-1
-
 Danio rerio
chondroitin 4-O-sulfotransferase-2
-
 Danio rerio

General Information

General Information Comment Organism
physiological function chondroitin 4-O-sulfotransferase-1 is required for somitic muscle development and motor axon guidance in zebrafish Danio rerio