Information on EC 2.8.2.5 - chondroitin 4-sulfotransferase

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The expected taxonomic range for this enzyme is: Euteleostomi

EC NUMBER
COMMENTARY
2.8.2.5
-
RECOMMENDED NAME
GeneOntology No.
chondroitin 4-sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
chondroitin sulfate biosynthesis (late stages)
-
Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:chondroitin 4'-sulfotransferase
The sulfation takes place at the 4-position of N-acetyl-galactosamine residues of chondroitin. Not identical with EC 2.8.2.17 chondroitin 6-sulfotransferase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C4ST
Q8NET6
-
C4ST
Q9NPF2, Q9NRB3
-
C4ST
Q9JME2
-
C4ST
-
-
C4ST-1
Q7T3S3
-
C4ST-2
Q75W66
-
chondroitin 4-O-sulfotransferase
Q9NPF2
-
chondroitin 4-O-sulfotransferase-1
Q7T3S3
-
chondroitin 4-O-sulfotransferase-1
-
-
chondroitin 4-O-sulfotransferase-1
Q9JME2
-
chondroitin 4-O-sulfotransferase-2
Q75W66
-
chondroitin 4-O-sulfotransferase-2
-
-
chondroitin 4-sulfotransferase 1
-
-
chondroitin 4-sulfotransferase 2
-
-
chondroitin sulfotransferase
-
-
-
-
CHST11
-
-
CS GalNAc 4-O-sulfotransferase
-
-
sulfotransferase, chondroitin
-
-
-
-
sulfotransferase, chondroitin 4-
-
-
-
-
mC4ST-1
Q9JME2
-
additional information
-
the enzyme belongs to the chondroitin/dermatan 4-O-sulfotransferase, C4ST/D4ST, family
CAS REGISTRY NUMBER
COMMENTARY
83589-04-2
-
9026-07-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
isozyme C4ST1
SwissProt
Manually annotated by BRENDA team
isozyme C4ST2
SwissProt
Manually annotated by BRENDA team
isozyme C4ST3
SwissProt
Manually annotated by BRENDA team
patient with B-cell chronic lyymphocytic leukemia
-
-
Manually annotated by BRENDA team
recombinant enzyme
-
-
Manually annotated by BRENDA team
C57BL/6 mice
-
-
Manually annotated by BRENDA team
isozymes C4ST-1 and C4ST-2
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
the enzyme regulates the length and amount of chondroitin sulfate chains in co-operation with chondroitin N-acetylgalactosaminyltransferase 2
metabolism
-
the enzyme plays a key role in regulating levels of chondroitin sulfate synthesized via chondroitin N-acetylgalactosaminyltransferase-1
physiological function
-, Q75W66, Q7T3S3
chondroitin 4-O-sulfotransferase-1 is required for somitic muscle development and motor axon guidance in zebrafish
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-, Q75W66, Q7T3S3
-
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
the enzyme is involved in the biosynthesis of dermatan sulfate in addition to chondroitin sulfate intermediates
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-, Q75W66, Q7T3S3
recombinant C4ST-1 shows a substrate preference for chondroitin and catalysed the 4-O-sulfation of GalNAc residues
-
-
?
3'-phosphoadenylyl sulfate + dermatan
adenosine 3',5'-bisphosphate + dermatan 4'-sulfate
show the reaction diagram
-
-, the enzyme is involved in the biosynthesis of dermatan sulfate in addition to chondroitin sulfate intermediates
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-, Q9NPF2, Q9NRB3
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
Q8NET6
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
Q9JME2
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
isozyme C4ST1, best substrate
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
involved in biosynthesis of chondroitin sulfate
-
?
3'-phosphoadenylylsulfate + chondroitin sulfate A
adenosine 3',5'-bisphosphate + chondroitin sulfate A 4'-sulfate
show the reaction diagram
-, Q9NPF2, Q9NRB3
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin sulfate A
adenosine 3',5'-bisphosphate + chondroitin sulfate A 4'-sulfate
show the reaction diagram
-
low activity
-
-
?
3'-phosphoadenylylsulfate + chondroitin sulfate A
adenosine 3',5'-bisphosphate + chondroitin sulfate A 4'-sulfate
show the reaction diagram
-
low activity
-
-
?
3'-phosphoadenylylsulfate + chondroitin sulfate B
adenosine 3',5'-bisphosphate + chondroitin sulfate B 4'-sulfate
show the reaction diagram
-
low activity
-
-
?
3'-phosphoadenylylsulfate + chondroitin sulfate C
adenosine 3',5'-bisphosphate + chondroitin sulfate C 4'-sulfate
show the reaction diagram
-, Q9NPF2, Q9NRB3
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin sulfate C
adenosine 3',5'-bisphosphate + chondroitin sulfate C 4'-sulfate
show the reaction diagram
-
low activity
-
-
?
3'-phosphoadenylylsulfate + chondroitin sulfate C
adenosine 3',5'-bisphosphate + chondroitin sulfate C 4'-sulfate
show the reaction diagram
-
low activity
-
-
?
3'-phosphoadenylylsulfate + chondroitin sulfate D
adenosine 3',5'-bisphosphate + chondroitin sulfate D 4'-sulfate
show the reaction diagram
-
low activity
-
-
?
3'-phosphoadenylylsulfate + chondroitin-derived oligosaccharides
adenosine 3',5'-bisphosphate + mixture of 4'-monosulfated oligosaccharides
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin-derived oligosaccharides
adenosine 3',5'-bisphosphate + mixture of 4'-monosulfated oligosaccharides
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
?
show the reaction diagram
-, Q9NPF2, Q9NRB3
-
-
-
?
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
?
show the reaction diagram
Q8NET6
-
-
-
?
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
?
show the reaction diagram
Q9JME2
-
-
-
?
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
?
show the reaction diagram
Q8NET6
i.e. dermatan
-
-
?
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
113% of the activity with chondroitin
-
-
?
3'-phosphoadenylylsulfate + partially desulfated dermatan sulfate
?
show the reaction diagram
-
-, isozyme C4ST2, best substrate, isozyme C4ST3, very low activity
-
-
?
additional information
?
-
-
product structure analysis
-
-
-
additional information
?
-
-
no activity with dermatan sulfate, keratan sulfate
-
-
-
additional information
?
-
-, Q9NPF2, Q9NRB3
no activity with dermatan sulfate, keratan sulfate
-
-
-
additional information
?
-
-
chondroitin sulfate E is no substrate
-
-
-
additional information
?
-
-
chondroitin sulfate E, heparan sulfate, and completely desulfated N-resulfated heparin are no acceptor substrates
-
-
-
additional information
?
-
-, Q9NPF2, Q9NRB3
no activity with desulfated and N-sulfated heparin, and desulfated and N-acetylated heparin
-
-
-
additional information
?
-
-
enzyme is required for proper chondroitin sulfate localization, modulation of distinct signaling pathways and cartilage growth plate morphogenesis
-
-
-
additional information
?
-
-
no substrate: chondroitin sulfate A, chondroitin sulfate C, dermatan sulfate. Enzyme mainly transfers sulfate to position 4 of the GalNAc residue located at the GlcA-GalNAc-GlcA sequence
-
-
-
additional information
?
-
-
the enzyme regulates E disaccharide expression of chondroitin sulfate required for Herpes simplex virus infectivity in mouse cells, overview
-
-
-
additional information
?
-
-, Q75W66, Q7T3S3
C4ST-1 also shows activity with partially desulfated dermatan sulfate, strong preference of zebrafish C4ST-1 for the sequence -GlcA-GalNAc(4S)-GlcA- over -IdoA-GalNAc(4S)-IdoA-
-
-
-
additional information
?
-
-, Q75W66, Q7T3S3
C4ST-2 also shows activity with partially desulfated dermatan sulfate
-
-
-
additional information
?
-
-
chondroitin 4-sulfotransferase-1 and -2, which catalyze the 4-O-sulfation of nonsulfated GalNAc residues
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-, Q75W66, Q7T3S3
-
-
-
?
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
the enzyme is involved in the biosynthesis of dermatan sulfate in addition to chondroitin sulfate intermediates
-
-
?
3'-phosphoadenylyl sulfate + dermatan
adenosine 3',5'-bisphosphate + dermatan 4'-sulfate
show the reaction diagram
-
the enzyme is involved in the biosynthesis of dermatan sulfate in addition to chondroitin sulfate intermediates
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
involved in biosynthesis of chondroitin sulfate
-
?
additional information
?
-
-
enzyme is required for proper chondroitin sulfate localization, modulation of distinct signaling pathways and cartilage growth plate morphogenesis
-
-
-
additional information
?
-
-
the enzyme regulates E disaccharide expression of chondroitin sulfate required for Herpes simplex virus infectivity in mouse cells, overview
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ba2+
-
stimulates
Ca2+
-
highest stimulatory effect, optimal concentration: 5 mM
Fe2+
-
stimulates
Mg2+
-
stimulates, to a lesser intent than Mn2+
Mg2+
-
slightly stimulating
Mn2+
-
optimum concentration: 1-10 mM; stimulates
Mn2+
-
stimulates
Mn2+
-
stimulates
Sr2+
-
stimulates
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-(N-Morpholino)ethanesulfonic acid buffer
-
pH above 6.0
Detergents
-
with the exception of Triton X-100 and Nonidet P-40, inhibit sulfation in the mast cell system
Oversulfated glucosaminoglycan
-
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
optimum concentration: 10 mM; stimulates
2-mercaptoethanol
-
stimulates
Basic proteins
-
stimulate
-
dithiothreitol
-
stimulates, optimum concentration: 2.0 mM
dithiothreitol
-
stimulation only at pH 6.0
dithiothreitol
-
activation of the purified enzyme
glutathione
-
stimulates, optimum concentration: 5.0 mM
glutathione
-
reduced glutathione stimulates
Histone
-
stimulates, optimum concentration: 0.5 mg/ml
Nonidet P-40
-
activates at 1%
Polyamines
-
stimulate
-
protamine
-
optimum concentration: 0.075 mg/ml; stimulates
protamine
-
molar ratio of protamine to repeating disaccharide unit of chondroitin is 1:100; stimulates
protamine
-
stimulates
protamine
-
stimulates
spermidine
-
stimulates, optimum concentration: 4.0 mM
spermine
-
optimum concentration: 0.6 mM; stimulates
spermine
-
stimulates
Triton X-100
-
activates at 1%
Triton X-100
-
increases activity in mast cell system
Lysozyme
-
stimulates, optimum concentration: 4.0 mg/ml
-
additional information
-
stimulation by basic substances is much higher than that by Mn2+, however increasing Mn2+ concentration immediately reduces the stimulation by basic substances
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00027
-
3'-phosphoadenylylsulfate
-
pH 6.8, 37C, in presence of protamine
0.0005
-
3'-phosphoadenylylsulfate
-
pH 6.8, 37C, wild-type
0.0011
-
3'-phosphoadenylylsulfate
-
pH 6.8, 37C, mutant with deletion of C-terminal glycosylation site
0.3
-
3'-phosphoadenylylsulfate
-
pH 6.8, 37C, in presence of protamine
0.8
-
3'-phosphoadenylylsulfate
-
pH 6.8, 37C, in addition of spermine
1.4
-
3'-phosphoadenylylsulfate
-
pH 6.8, 37C, in addition of Mn2+
2.1
-
Chondroitin
-
pH 6.8, 37C, wild-type
2.7
-
Chondroitin
-
pH 6.8, 37C, mutant with deletion of C-terminal glycosylation site
additional information
-
additional information
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.000006
-
-
purified enzyme
0.00001
-
-
recombinant enzyme, substrate chondroitin
additional information
-
-
-
additional information
-
-
quantitative expression analysis of C4ST-1 and C4ST-2
additional information
-
-, Q75W66, Q7T3S3
recombinant enzyme; recombinant enzyme activity with chondroitin and partially desulfated dermatan sulfate
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
7.5
-
-
6.2
-
-
-
6.4
-
-
-
6.5
-
-
assay at
6.5
-
-
-
6.8
-
-
assay at; assay at
6.8
-
-
assay at
6.8
-
-
assay at; assay at
7.5
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
28
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at
37
-
-
assay at; assay at
37
-
-
assay at
37
-
-
assay at; assay at
37
-
-
assay at
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
isozyme C4ST3 shows very poor activity with chondroitin and partially desulfated dermatan sulfate as substrates at 37C compared to 28C
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
bone marrow-derived mast cell, 200fold higher expression of mC4ST-1 than in bone marrow cell
Manually annotated by BRENDA team
-
C4ST-2 expression occurs in the early stage bone marrow cell cultures, the process of mast cell maturation does not alter the expression, low expression at day 0, followed by an increase in expression at later stages of culture
Manually annotated by BRENDA team
-
C4ST-1 and C4ST-2 are ubiquitously expressed in the postnatal mouse brain, tissue distribution of expression, overview
Manually annotated by BRENDA team
-, Q75W66, Q7T3S3
-
Manually annotated by BRENDA team
-
embryonic; epiphyseal
Manually annotated by BRENDA team
-
chondrosarcoma cell line
Manually annotated by BRENDA team
-
cell culture; embryo; extracellular, secretion to the medium
Manually annotated by BRENDA team
-
cell culture; embryo
Manually annotated by BRENDA team
-
extracellular, secretion to the medium
Manually annotated by BRENDA team
-, Q75W66, Q7T3S3
;
Manually annotated by BRENDA team
-
low content
Manually annotated by BRENDA team
-
isozyme C4ST1 expression
Manually annotated by BRENDA team
-
isozyme C4ST3, high content in adult, low content in fetal liver
Manually annotated by BRENDA team
-
adult and fetal; isozyme C4ST1 expression; isozyme C4ST2 expression
Manually annotated by BRENDA team
-
isozyme C4ST1 expression
Manually annotated by BRENDA team
-
; isozyme C4ST, low content
Manually annotated by BRENDA team
-
developed from bone marrow cells isolated from femur and tibia from C57BL/6 mice, mast cell maturation strongly correlates with the expression of C4ST-1
Manually annotated by BRENDA team
-, Q75W66, Q7T3S3
;
Manually annotated by BRENDA team
-, Q75W66, Q7T3S3
-
Manually annotated by BRENDA team
-
isozyme C4ST2 expression
Manually annotated by BRENDA team
-
isozyme C4ST1 expression
Manually annotated by BRENDA team
-
isozyme C4ST2 expression
Manually annotated by BRENDA team
-, Q75W66, Q7T3S3
-
Manually annotated by BRENDA team
-
in hepatitis serum 4fold more active than in normal
Manually annotated by BRENDA team
-
isozyme C4ST2 expression
Manually annotated by BRENDA team
-, Q75W66, Q7T3S3
-
Manually annotated by BRENDA team
-
isozyme C4ST2 expression
Manually annotated by BRENDA team
-
isozyme C4ST1 expression; isozyme C4ST2 expression
Manually annotated by BRENDA team
-
ventricular zone of the dorsal and the ventral telencephalon
Manually annotated by BRENDA team
-, Q75W66, Q7T3S3
-
Manually annotated by BRENDA team
-
isozyme C4ST1 expression
Manually annotated by BRENDA team
-
of the telencephalon
Manually annotated by BRENDA team
additional information
-
quantitative expression analysis
Manually annotated by BRENDA team
additional information
-, Q75W66, Q7T3S3
C4ST-1 expression pattern, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
enzyme is released into the cell culture medium; secretion into the medium is supported by ascorbic acid and inhibited by monensin
-
Manually annotated by BRENDA team
-
enzyme is released into the cell culture medium
-
Manually annotated by BRENDA team
-
secreted to the extracellular space in a soluble form under the culture conditions
Manually annotated by BRENDA team
additional information
-
type II transmembrane protein
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50000
-
-
about, gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
-
1 * 35000, deglycosylated protein, SDS-PAGE; 1 * 50000-54000, nonreducing conditions, SDS-PAGE; 1 * 60000-64000, reducing conditions, SDS-PAGE
additional information
-
the deglycosylated enzyme forms dimers in solution of 70 kDa
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
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enzyme contains 4 potential N-glycosylation sites; enzyme contains N-linked oligosaccharides
glycoprotein
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enzyme contains N-linked oligosaccharides
glycoprotein
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contains about 35% N-linked oligosaccharides, treatment of enzyme with peptide N-glycosidase F causes a marked decrease in activity, production of active form of recombinant enzyme is inhibited by tunicamycin
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, purified enzyme, loss of 80% activity
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant His-tagged isozyme C4ST3 from CHO cells
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sephadex G-25 chromatography
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1920fold, to homogeneity, from the serum-free cell culture medium
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
gene C4ST-1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis; gene C4ST-2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
-, Q75W66, Q7T3S3
DNA sequence determination and analysis, chromosome mapping at p22 region of chromosome 7, expression in CHO cells; DNA sequence dtermination and analysis, chromosome mapping at q23 region of chromosome 12, expression in CHO cells
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DNA sequence determination and analysis, located on chromosome 3, genomic organisation, expression as myc- and His-tagged enzyme in CHO cells
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expression as N-terminally truncated enzyme, lacking the first 57 amino acids, in COS-1 cells; expression as N-terminally truncated enzyme, lacking the first 76 amino acids, in COS-1 cells
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expressed in L cells
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expression analysis
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expression of C4ST-1 in enzyme-deficient sog9 cells
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genetic library screening, DNA and amino acid sequence determination and analysis, transient functional overexpression of wild-type and FLAG-tagged enzyme in COS-7 cells, control expression of C4ST in antisense orientation
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genes C4ST-1 and C4ST-2, quantitative expression analysis
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EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
A23187 causes a down-regulated expression of the C4ST-2 gene with 50% reduction after 1 h, followed by return to basal levels after 4.5 h
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ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-, Q75W66, Q7T3S3
functional knockdown of C4ST-1 with specific antisense morpholino-oligonucleotides leads to a marked decrease in the 4-O-sulfation and amount of chondroitin sulfate in the embryos. Consistent with the preferential expression in the rostrocaudal axis, C4ST-1 morphants display morphological defects exemplified by a ventrally bent trunk and a curled and/or kinky tail, largely due to misregulated myotomal myod expression, implying perturbation of axial muscle differentiation in somites. Rescue of morphological phenotypes of the MO1-injected embryos by injection with zebrafish C4ST-1 RNA, phenotypes, overview
additional information
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translocations t(12,14)(q23,q32) in a patient with B-cell chronic lymphocytic leukemia result in expression of two truncated forms of enzyme protein, resulting in disturbance of the cellular distribution of enzyme, and further in deregulation of a chondroitin-sulfate dependent pathway specific to the hematopoietic lineage
additional information
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gene-trap mutation in bone morphogenic protein-induced enzyme causes severe chondrodysplasia characterised by disorganised cartilage growth plate as well as specific alterations in the orientation of chondrocyte columns. Phenotype is associated with chondroitin sulfation imbalance, mislocalization of chondroitin sulfate and imbalance of apoptotic signals
additional information
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CS-deficient sog9 cells, derived from gro2C cells partially resistant to HSV-1 infection and defective in the expression of heparan sulfate because of a splice site mutation in the EXT1 gene encoding the HS-synthesizing enzyme, have a defect in the expression of C4ST-1 in addition to EXT1, decrease in the amount of chondroitin sulfate in sog9 cells is the result of a reduction in the length of chondroitin sulfate chains, glycosyltransferases participating in the biosynthesis of chondroitin sulfate are not affected in sog9 cells, rescue of sog9 cells only by introducing C4ST-1
R186A
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the mutant fails to bind 3'-phosphoadenylyl sulfate
additional information
-
deletion of N-glycosylation sites, loss of the N-glycosylation site at the C-terminus reduces activity by 80%
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
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translocations t(12,14)(q23,q32) in a patient with B-cell chronic lymphocytic leukemia result in expression of two truncated forms of enzyme protein, resulting in disturbance of the cellular distribution of enzyme, and further in deregulation of a chondroitin-sulfate dependent pathway specific to the hematopoietic lineage
medicine
-
gene-trap mutation in bone morphogenic protein-induced enzyme causes severe chondrodysplasia characterised by disorganised cartilage growth plate as well as specific alterations in the orientation of chondrocyte columns. Phenotype is associated with chondroitin sulfation imbalance, mislocalization of chondroitin sulfate and imbalance of apoptotic signals