Information on EC 2.8.2.5 - chondroitin 4-sulfotransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.8.2.5
-
RECOMMENDED NAME
GeneOntology No.
chondroitin 4-sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
chondroitin sulfate biosynthesis (late stages)
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-
Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
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-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:chondroitin 4'-sulfotransferase
The sulfation takes place at the 4-position of N-acetyl-galactosamine residues of chondroitin. Not identical with EC 2.8.2.17 chondroitin 6-sulfotransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
83589-04-2
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9026-07-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
C57BL/6 mice
-
-
Manually annotated by BRENDA team
isozymes C4ST-1 and C4ST-2
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + dermatan
adenosine 3',5'-bisphosphate + dermatan 4'-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + chondroitin sulfate A
adenosine 3',5'-bisphosphate + chondroitin sulfate A 4'-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + chondroitin sulfate B
adenosine 3',5'-bisphosphate + chondroitin sulfate B 4'-sulfate
show the reaction diagram
-
low activity
-
-
?
3'-phosphoadenylylsulfate + chondroitin sulfate C
adenosine 3',5'-bisphosphate + chondroitin sulfate C 4'-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + chondroitin sulfate D
adenosine 3',5'-bisphosphate + chondroitin sulfate D 4'-sulfate
show the reaction diagram
-
low activity
-
-
?
3'-phosphoadenylylsulfate + chondroitin-derived oligosaccharides
adenosine 3',5'-bisphosphate + mixture of 4'-monosulfated oligosaccharides
show the reaction diagram
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
?
show the reaction diagram
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
113% of the activity with chondroitin
-
-
?
3'-phosphoadenylylsulfate + partially desulfated dermatan sulfate
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + dermatan
adenosine 3',5'-bisphosphate + dermatan 4'-sulfate
show the reaction diagram
-
the enzyme is involved in the biosynthesis of dermatan sulfate in addition to chondroitin sulfate intermediates
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
stimulates
Ca2+
-
highest stimulatory effect, optimal concentration: 5 mM
Fe2+
-
stimulates
Sr2+
-
stimulates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-(N-Morpholino)ethanesulfonic acid buffer
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pH above 6.0
Detergents
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with the exception of Triton X-100 and Nonidet P-40, inhibit sulfation in the mast cell system
heparin
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monensin
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Oversulfated glucosaminoglycan
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-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
Basic proteins
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stimulate
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dithiothreitol
glutathione
histone
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stimulates, optimum concentration: 0.5 mg/ml
lysozyme
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stimulates, optimum concentration: 4.0 mg/ml
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Nonidet P-40
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activates at 1%
Polyamines
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stimulate
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protamine
spermidine
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stimulates, optimum concentration: 4.0 mM
spermine
Triton X-100
additional information
-
stimulation by basic substances is much higher than that by Mn2+, however increasing Mn2+ concentration immediately reduces the stimulation by basic substances
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00027 - 1.4
3'-phosphoadenylylsulfate
2.1 - 2.7
Chondroitin
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000006
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purified enzyme
0.00001
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recombinant enzyme, substrate chondroitin
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7.5
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-
7.5
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assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28
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assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
isozyme C4ST3 shows very poor activity with chondroitin and partially desulfated dermatan sulfate as substrates at 37C compared to 28C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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bone marrow-derived mast cell, 200fold higher expression of mC4ST-1 than in bone marrow cell
Manually annotated by BRENDA team
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embryonic; epiphyseal
Manually annotated by BRENDA team
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low content
Manually annotated by BRENDA team
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isozyme C4ST1 expression
Manually annotated by BRENDA team
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isozyme C4ST3, high content in adult, low content in fetal liver
Manually annotated by BRENDA team
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adult and fetal; isozyme C4ST1 expression; isozyme C4ST2 expression
Manually annotated by BRENDA team
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isozyme C4ST2 expression
Manually annotated by BRENDA team
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isozyme C4ST1 expression
Manually annotated by BRENDA team
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isozyme C4ST2 expression
Manually annotated by BRENDA team
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in hepatitis serum 4fold more active than in normal
Manually annotated by BRENDA team
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isozyme C4ST2 expression
Manually annotated by BRENDA team
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isozyme C4ST2 expression
Manually annotated by BRENDA team
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isozyme C4ST1 expression; isozyme C4ST2 expression
Manually annotated by BRENDA team
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isozyme C4ST1 expression
Manually annotated by BRENDA team
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of the telencephalon
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
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type II transmembrane protein
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
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1 * 35000, deglycosylated protein, SDS-PAGE
50000
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about, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 35000, deglycosylated protein, SDS-PAGE; 1 * 50000-54000, nonreducing conditions, SDS-PAGE; 1 * 60000-64000, reducing conditions, SDS-PAGE
additional information
-
the deglycosylated enzyme forms dimers in solution of 70 kDa
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, purified enzyme, loss of 80% activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1920fold, to homogeneity, from the serum-free cell culture medium
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cobalt resin column chromatography
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IgG-Sepharose bead chromatography
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recombinant His-tagged isozyme C4ST3 from CHO cells
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sephadex G-25 chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, chromosome mapping at p22 region of chromosome 7, expression in CHO cells; DNA sequence dtermination and analysis, chromosome mapping at q23 region of chromosome 12, expression in CHO cells
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DNA sequence determination and analysis, located on chromosome 3, genomic organisation, expression as myc- and His-tagged enzyme in CHO cells
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expressed in COS-1 cells
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expressed in HEK-293 cells
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expressed in L cells
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expression analysis
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expression as N-terminally truncated enzyme, lacking the first 57 amino acids, in COS-1 cells; expression as N-terminally truncated enzyme, lacking the first 76 amino acids, in COS-1 cells
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expression of C4ST-1 in enzyme-deficient sog9 cells
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gene C4ST-1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis; gene C4ST-2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
genes C4ST-1 and C4ST-2, quantitative expression analysis
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genetic library screening, DNA and amino acid sequence determination and analysis, transient functional overexpression of wild-type and FLAG-tagged enzyme in COS-7 cells, control expression of C4ST in antisense orientation
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
a decline in arylsulfatase B reduces chondroitin-4-sulfotransferase mRNA expression. Inhibition of bone morphogenetic protein 4 also reduces enzyme expression
a significantly higher mRNA expression of CHST11 is measured in ovarian cancer samples in comparison to non-malignant ones
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A23187 causes a down-regulated expression of the C4ST-2 gene with 50% reduction after 1 h, followed by return to basal levels after 4.5 h
silencing Wnt9A increases the expression of CHST11
the suppression of enzyme gene expression by Wnt/b-etacatenin signaling can be recovered by treatment with trichostatin A
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traumatic brain injury leads to an increase in mRNA expression
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R186A
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the mutant fails to bind 3'-phosphoadenylyl sulfate
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine