Information on EC 2.8.2.5 - chondroitin 4-sulfotransferase

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The expected taxonomic range for this enzyme is: Euteleostomi

EC NUMBER
COMMENTARY hide
2.8.2.5
-
RECOMMENDED NAME
GeneOntology No.
chondroitin 4-sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
chondroitin sulfate biosynthesis (late stages)
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-
Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
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-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:chondroitin 4'-sulfotransferase
The sulfation takes place at the 4-position of N-acetyl-galactosamine residues of chondroitin. Not identical with EC 2.8.2.17 chondroitin 6-sulfotransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
83589-04-2
-
9026-07-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
Mus musculus C57BL/6
C57BL/6 mice
-
-
Manually annotated by BRENDA team
isozymes C4ST-1 and C4ST-2
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
chondroitin 4-O-sulfotransferase-1 is required for somitic muscle development and motor axon guidance in zebrafish
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + dermatan
adenosine 3',5'-bisphosphate + dermatan 4'-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + chondroitin sulfate A
adenosine 3',5'-bisphosphate + chondroitin sulfate A 4'-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + chondroitin sulfate B
adenosine 3',5'-bisphosphate + chondroitin sulfate B 4'-sulfate
show the reaction diagram
-
low activity
-
-
?
3'-phosphoadenylylsulfate + chondroitin sulfate C
adenosine 3',5'-bisphosphate + chondroitin sulfate C 4'-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + chondroitin sulfate D
adenosine 3',5'-bisphosphate + chondroitin sulfate D 4'-sulfate
show the reaction diagram
-
low activity
-
-
?
3'-phosphoadenylylsulfate + chondroitin-derived oligosaccharides
adenosine 3',5'-bisphosphate + mixture of 4'-monosulfated oligosaccharides
show the reaction diagram
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
?
show the reaction diagram
3'-phosphoadenylylsulfate + desulfated dermatan sulfate
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
113% of the activity with chondroitin
-
-
?
3'-phosphoadenylylsulfate + partially desulfated dermatan sulfate
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + dermatan
adenosine 3',5'-bisphosphate + dermatan 4'-sulfate
show the reaction diagram
-
the enzyme is involved in the biosynthesis of dermatan sulfate in addition to chondroitin sulfate intermediates
-
-
?
3'-phosphoadenylylsulfate + chondroitin
adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
stimulates
Ca2+
-
highest stimulatory effect, optimal concentration: 5 mM
Fe2+
-
stimulates
Sr2+
-
stimulates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-(N-Morpholino)ethanesulfonic acid buffer
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pH above 6.0
Detergents
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with the exception of Triton X-100 and Nonidet P-40, inhibit sulfation in the mast cell system
heparin
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monensin
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Oversulfated glucosaminoglycan
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-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
Basic proteins
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stimulate
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dithiothreitol
glutathione
histone
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stimulates, optimum concentration: 0.5 mg/ml
lysozyme
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stimulates, optimum concentration: 4.0 mg/ml
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Nonidet P-40
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activates at 1%
Polyamines
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stimulate
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protamine
spermidine
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stimulates, optimum concentration: 4.0 mM
spermine
Triton X-100
additional information
-
stimulation by basic substances is much higher than that by Mn2+, however increasing Mn2+ concentration immediately reduces the stimulation by basic substances
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00027 - 1.4
3'-phosphoadenylylsulfate
2.1 - 2.7
Chondroitin
additional information
additional information
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000006
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purified enzyme
0.00001
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recombinant enzyme, substrate chondroitin
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7.5
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-
7.5
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assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28
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assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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isozyme C4ST3 shows very poor activity with chondroitin and partially desulfated dermatan sulfate as substrates at 37C compared to 28C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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bone marrow-derived mast cell, 200fold higher expression of mC4ST-1 than in bone marrow cell
Manually annotated by BRENDA team
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embryonic; epiphyseal
Manually annotated by BRENDA team
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low content
Manually annotated by BRENDA team
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isozyme C4ST1 expression
Manually annotated by BRENDA team
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isozyme C4ST3, high content in adult, low content in fetal liver
Manually annotated by BRENDA team
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adult and fetal; isozyme C4ST1 expression; isozyme C4ST2 expression
Manually annotated by BRENDA team
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isozyme C4ST2 expression
Manually annotated by BRENDA team
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isozyme C4ST1 expression
Manually annotated by BRENDA team
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isozyme C4ST2 expression
Manually annotated by BRENDA team
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in hepatitis serum 4fold more active than in normal
Manually annotated by BRENDA team
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isozyme C4ST2 expression
Manually annotated by BRENDA team
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isozyme C4ST2 expression
Manually annotated by BRENDA team
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isozyme C4ST1 expression; isozyme C4ST2 expression
Manually annotated by BRENDA team
-
isozyme C4ST1 expression
Manually annotated by BRENDA team
-
of the telencephalon
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
type II transmembrane protein
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
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about, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 35000, deglycosylated protein, SDS-PAGE; 1 * 50000-54000, nonreducing conditions, SDS-PAGE; 1 * 60000-64000, reducing conditions, SDS-PAGE
additional information
-
the deglycosylated enzyme forms dimers in solution of 70 kDa
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, purified enzyme, loss of 80% activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1920fold, to homogeneity, from the serum-free cell culture medium
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recombinant His-tagged isozyme C4ST3 from CHO cells
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sephadex G-25 chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, chromosome mapping at p22 region of chromosome 7, expression in CHO cells; DNA sequence dtermination and analysis, chromosome mapping at q23 region of chromosome 12, expression in CHO cells
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DNA sequence determination and analysis, located on chromosome 3, genomic organisation, expression as myc- and His-tagged enzyme in CHO cells
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expressed in L cells
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expression analysis
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expression as N-terminally truncated enzyme, lacking the first 57 amino acids, in COS-1 cells; expression as N-terminally truncated enzyme, lacking the first 76 amino acids, in COS-1 cells
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expression of C4ST-1 in enzyme-deficient sog9 cells
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gene C4ST-1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis; gene C4ST-2, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
genes C4ST-1 and C4ST-2, quantitative expression analysis
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genetic library screening, DNA and amino acid sequence determination and analysis, transient functional overexpression of wild-type and FLAG-tagged enzyme in COS-7 cells, control expression of C4ST in antisense orientation
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
A23187 causes a down-regulated expression of the C4ST-2 gene with 50% reduction after 1 h, followed by return to basal levels after 4.5 h
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R186A
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the mutant fails to bind 3'-phosphoadenylyl sulfate
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine