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Literature summary for 2.8.2.20 extracted from
- Fluorescent peptide sensors for tyrosylprotein sulfotransferase activity (2014), Anal. Biochem., 461, 1-6.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Sf9 insect cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3'-phosphoadenosine 5'-phosphate | - |
Homo sapiens |  |
| PSGL-1 peptide | - |
Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0018 | - |
[Y+3 peptide]-L-tyrosine | isoform TPST2, at pH 7.5 and 25°C | Homo sapiens | |
| 0.0019 | - |
[Y+3 peptide]-L-tyrosine | isoform TPST1, at pH 7.5 and 25°C | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement of divalent metal ions | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O60507 | - |
- |
| Homo sapiens | O60704 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + [PSGL-1 peptide]-L-tyrosine | - |
Homo sapiens | adenosine 3',5'-bisphosphate + [PSGL-1 peptide]-L-tyrosine-O-sulfate | - |
? | |
| 3'-phosphoadenylyl sulfate + [Y+3 peptide]-L-tyrosine | i.e. biotin-RDRRQATEYEYLDYDFLPETEPPP | Homo sapiens | adenosine 3',5'-bisphosphate + [Y+3 peptide]-L-tyrosine-O-sulfate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TPST1 | isoform | Homo sapiens |
| TPST2 | isoform | Homo sapiens |
| tyrosylprotein sulfotransferase | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.054 | - |
[Y+3 peptide]-L-tyrosine | isoform TPST1, at pH 7.5 and 25°C | Homo sapiens | |
| 0.065 | - |
[Y+3 peptide]-L-tyrosine | isoform TPST2, at pH 7.5 and 25°C | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
isoform TPST1, at pH 7.5 and 25°C | Homo sapiens | PSGL-1 peptide | |
| 0.0007 | - |
isoform TPST2, at pH 7.5 and 25°C | Homo sapiens | PSGL-1 peptide | |
| 0.0099 | - |
isoform TPST1, at pH 7.5 and 25°C | Homo sapiens | 3'-phosphoadenosine 5'-phosphate | |
| 0.011 | - |
isoform TPST2, at pH 7.5 and 25°C | Homo sapiens | 3'-phosphoadenosine 5'-phosphate | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 29 | - |
[Y+3 peptide]-L-tyrosine | isoform TPST1, at pH 7.5 and 25°C | Homo sapiens | |
| 37 | - |
[Y+3 peptide]-L-tyrosine | isoform TPST2, at pH 7.5 and 25°C | Homo sapiens |
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