Cloned (Comment) | Organism |
---|---|
expression of isozyme TPST-1 in HEK-293T cells, quantitative real-time PCR expression analysis | Homo sapiens |
expression of isozyme TPST-2 in HEK-293T cells, quantitative real-time PCR expression analysis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Homo sapiens | |
0.0097 | - |
PSGL-1 | pH 6.0, 30°C, isozyme TPST-1 | Homo sapiens | |
0.0175 | - |
Tyr-Tyr-Tyr | pH 6.0, 30°C, isozyme TPST-1 | Homo sapiens | |
0.022 | - |
Tyr-Tyr-Tyr | pH 6.0, 30°C, isozyme TPST-2 | Homo sapiens | |
0.0269 | - |
PSGL-1 | pH 6.0, 30°C, isozyme TPST-2 | Homo sapiens | |
1.223 | - |
benzyl-Glu-Tyr | pH 6.0, 30°C, isozyme TPST-2 | Homo sapiens | |
1.297 | - |
benzyl-Glu-Tyr | pH 6.0, 30°C, isozyme TPST-1 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | TPST-1 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain | Homo sapiens | 5829 | - |
cytosol | TPST-2 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain | Homo sapiens | 5829 | - |
membrane | TPST-1 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain | Homo sapiens | 16020 | - |
membrane | TPST-2 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates isozyme TPST-2 | Homo sapiens | |
Mn2+ | activates isozymes TPST-1 | Homo sapiens | |
additional information | no activation of isozyme TPST-1 by Mg2+ | Homo sapiens | |
additional information | no activation of isozyme TPST-2 by Mn2+ | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + protein tyrosine | Homo sapiens | - |
adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O60507 | TPST-1; isozyme TPST-1 | - |
Homo sapiens | O60704 | TPST-2; isozyme TPST-2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | TPST-1 has two putative N-glycosylation sites | Homo sapiens |
glycoprotein | TPST-2 has two putative N-glycosylation sites | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | adult and fetal | Homo sapiens | - |
heart | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
additional information | broad tissue distribution of isozyme TPST-1, overview | Homo sapiens | - |
additional information | broad tissue distribution of isozyme TPST-2, overview | Homo sapiens | - |
neuron | - |
Homo sapiens | - |
placenta | - |
Homo sapiens | - |
prostate gland | - |
Homo sapiens | - |
salivary gland | - |
Homo sapiens | - |
spinal cord | - |
Homo sapiens | - |
testis | - |
Homo sapiens | - |
thymus | - |
Homo sapiens | - |
uterus | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000031 | - |
recombinant TPST-1 in HEK-293T cell extract | Homo sapiens |
0.0000032 | - |
recombinant TPST-2 in HEK-293T cell extract | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + benzyl-Glu-Tyr | - |
Homo sapiens | adenosine 3',5'-bisphosphate + benzyl-Glu-Tyr-O-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + protein tyrosine | - |
Homo sapiens | adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + protein tyrosine | high activity, isozyme TPST-2 | Homo sapiens | adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + protein tyrosine | low activity, isozyme TPST-1 | Homo sapiens | adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + PSGL-1 | - |
Homo sapiens | adenosine 3',5'-bisphosphate + PSGL-1 sulfate | - |
? | |
3'-phosphoadenylyl sulfate + Tyr-Tyr-Tyr | - |
Homo sapiens | adenosine 3',5'-bisphosphate + Tyr-Tyr-Tyr-O-sulfate | - |
? | |
additional information | activity with peptides encompassing tyrosine sulfation sites of chemokine receptors, CCR2, CCR3, CCR5, CCR8, cholecystokinin CCK, and complement C4, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | TPST-1 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain | Homo sapiens |
More | TST-2 displays type II transmembrane topology with a short N-terminal cytoplasmic domain, a single 17-residue transmembrane domain, and a luminal catalytic domain | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TPST-1 | - |
Homo sapiens |
TPST-2 | - |
Homo sapiens |
tyrosylprotein sulfotransferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
TPST-2 | Homo sapiens |
6.5 | - |
TPST-1 | Homo sapiens |