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Literature summary for 2.8.2.2 extracted from
- Isolation of two distinct 3-hydroxysteroid sulfotransferases from the guinea pig adrenal. Evidence for 3alpha-hydroxy versus 3beta-hydroxy stereospecificity (1993), J. Biol. Chem., 268, 23496-23503.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000083 | - |
allopregnanolone | pH 7.7, 37°C | Cavia porcellus |  |
| 0.000175 | - |
androsterone | pH 7.7, 37°C | Cavia porcellus | |
| 0.000257 | - |
pregnenolone | pH 7.7, 37°C | Cavia porcellus | |
| 0.000897 | - |
17-hydroxypregnenolone | pH 7.7, 37°C | Cavia porcellus | |
| 0.001157 | - |
dehydroepiandrosterone | pH 7.7, 37°C | Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Cavia porcellus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
isoenzyme 3alpha-HAST, SDS-PAGE | Cavia porcellus |
| 33000 | - |
isoenzyme 3beta-HAST, SDS-PAGE | Cavia porcellus |
| 135000 | - |
gel filtration | Cavia porcellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | Cavia porcellus | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
guinea pig | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Cavia porcellus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylylsulfate + 17-hydroxypregnenolone | 3beta,17alpha-dihydroxypregn-4-en-3,20-dione, isoenzyme 3beta-HST | Cavia porcellus | adenosine 3',5'-bisphosphate + (3beta)-17-hydroxy-20-oxopregn-5-en-3-yl sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + allo-pregnanolone | isoenzyme 3alpha-HST | Cavia porcellus | adenosine 3',5'-bisphosphate + allo-pregnanolone sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + androsterone | 3alpha-hydroxy-5alpha-androstan-17-one | Cavia porcellus | adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + androsterone | isozyme 3alpha-HST | Cavia porcellus | adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + beta-estradiol | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + beta-estradiol 3-O-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | - |
Cavia porcellus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + dehydroepiandrosterone | isoenzyme 3beta-HST | Cavia porcellus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
r | |
| 3'-phosphoadenylylsulfate + pregnenolone | isoenzyme 3beta-HST | Cavia porcellus | adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate | - |
r | |
| additional information | 2 isoenzymes of 3-hydroxysteroid sulfotransferase | Cavia porcellus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Cavia porcellus |
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