Information on EC 2.8.2.2 - alcohol sulfotransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.8.2.2
-
RECOMMENDED NAME
GeneOntology No.
alcohol sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + an alcohol = adenosine 3',5'-bisphosphate + an alkyl sulfate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Steroid hormone biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:alcohol sulfotransferase
Primary and secondary alcohols, including aliphatic alcohols, ascorbic acid, chloramphenicol, ephedrine and hydroxysteroids, but not phenolic steroids, can act as acceptors (cf. EC 2.8.2.15 steroid sulfotransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
9032-76-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
guinea pig
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
hamster
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-
Manually annotated by BRENDA team
rabbit
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
-
SULT1B1
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5'-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
best substrate
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5,5'-tetraiodothyroacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5-triiodothyroacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,5-diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3-iodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 5alpha,6alpha-epoxycholesterol
adenosine 3',5'-bisphosphate + 5alpha,6alpha-epoxycholesteryl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 5beta,6beta-epoxycholesterol
adenosine 3',5'-bisphosphate + 5beta,6beta-epoxycholesteryl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 7-oxo-cholesterol
adenosine 3',5'-bisphosphate + 7-oxo-cholesteryl 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 7-oxocholesterol
adenosine 3',5'-bisphosphate + 7-oxocholesterol 3-sulfate
show the reaction diagram
SULT2B1b, SULT2B1a, and SULT2A1
-
-
?
3'-phosphoadenylyl sulfate + 7alpha-hydroxycholesterol
adenosine 3',5'-bisphosphate + 7alpha-hydroxycholesterol 3-sulfate
show the reaction diagram
SULT2B1b, the 7alpha derivative is highly preferred
-
-
?
3'-phosphoadenylyl sulfate + 7alpha-hydroxycholesterol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 7beta-hydroxycholesterol
adenosine 3',5'-bisphosphate + 7beta-hydroxycholesterol 3-sulfate
show the reaction diagram
SULT2B1b, the 7alpha derivative is highly preferred
-
-
?
3'-phosphoadenylyl sulfate + 7beta-hydroxycholesterol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + androsterone
adenosine 3',5'-bisphosphate + androsterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + aristolochic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + cholesterol 7alpha-hydroperoxide
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
SULT2B1b
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + genistein
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + raloxifene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + tibolone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + (R)-(-)-2-heptanol
adenosine 3',5'-bisphosphate + (R)-(-)-2-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + (S)-(+)-2-heptanol
adenosine 3',5'-bisphosphate + (S)-(+)-2-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + (S)-(-)-1-phenyl-1-heptanol
adenosine 3',5'-bisphosphate + (S)-(-)-1-phenyl-1-heptyl sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + 1'-hydroxysafrole
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation to a lesser extent
-
-
?
3'-phosphoadenylylsulfate + 1-butanol
adenosine 3',5'-bisphosphate + 1-butanoyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 1-decanol
adenosine 3',5'-bisphosphate + 1-decyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-dodecanol
adenosine 3',5'-bisphosphate + 1-dodecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-heptanol
adenosine 3',5'-bisphosphate + 1-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-hexanol
adenosine 3',5'-bisphosphate + 1-hexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-hydroxymethylpyrene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation
-
-
?
3'-phosphoadenylylsulfate + 1-methylcyclohexanol
adenosine 3',5'-bisphosphate + 1-methylcyclohexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-nonanol
adenosine 3',5'-bisphosphate + 1-nonyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-octanol
adenosine 3',5'-bisphosphate + 1-octyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-pentanol
adenosine 3',5'-bisphosphate + 1-pentyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-propanol
adenosine 3',5'-bisphosphate + 1-propyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-tetradecanol
adenosine 3',5'-bisphosphate + 1-tetradecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-undecanol
adenosine 3',5'-bisphosphate + 1-undecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 11-deoxycorticosterone
adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 17-hydroxypregnenolone
adenosine 3',5'-bisphosphate + pregn-4-en-3,20-dione 3-sulfate
show the reaction diagram
-
3beta,17alpha-dihydroxypregn-4-en-3,20-dione, isoenzyme 3beta-HST
-
-
r
3'-phosphoadenylylsulfate + 17alpha-ethinylestradiol
adenosine 3',5'-bisphosphate + 17alpha-ethinylestradiol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-hydroxy-catecholestrogen
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
best substrate for this enzyme, also catalyzes the transformation of 4-hydroxy-estrogens and 16-hydroxy-estrogens, but with a lower affinity
-
-
r
3'-phosphoadenylylsulfate + 2-methyl-2-hexanol
adenosine 3',5'-bisphosphate + 1,1-dimethylpentyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-propanol
adenosine 3',5'-bisphosphate + 2-propyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3,3'-diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
3'-phosphoadenylylsulfate + 3,5-diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
3'-phosphoadenylylsulfate + 3-ethyl-3-pentanol
adenosine 3',5'-bisphosphate + 3-ethyl-3-pentyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3-keto-5alpha-petromyzonol
adenosine 3',5'-bisphosphate + 7alpha,12alpha,24-trihydroxy-5alpha-cholan-3-one 24 sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 3-methyl-1-butanol
adenosine 3',5'-bisphosphate + 3-methyl-1-butyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3-O-phenyl-17beta-estradiol
adenosine 3',5'-bisphosphate + 3-phenolic hydroxyl beta-estradiol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3alpha-hydroxy-5beta-androstan-17-one
adenosine 3',5'-bisphosphate + 5beta-androstan-17-one 3-sulfate
show the reaction diagram
-
relative activity 5%
-
-
?
3'-phosphoadenylylsulfate + 3beta,17alpha-dihydroxy-pregn-5-ene-20-one
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 4%
-
-
r
3'-phosphoadenylylsulfate + 3beta-hydroxy-5beta-androstan-17-one
adenosine 3',5'-bisphosphate + 5beta-androstan-17-one 3-sulfate
show the reaction diagram
-
relative activity 45%
-
-
r
3'-phosphoadenylylsulfate + 4,4'-isopropylidenediphenol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 4-(1,1,3,3-tetramethylbutyl)phenol
adenosine 3',5'-bisphosphate + 4-(1,1,3,3-tetramethylbutyl)phenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 4-butylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-butylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-ethylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-ethylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-heptanol
adenosine 3',5'-bisphosphate + 4-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-heptylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-heptylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-hexylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-hexylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-methylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-methylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-methylumbelliferone
adenosine 3',5'-bisphosphate + 4-methylumbelliferone sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + 4-n-nonylphenol
adenosine 3',5'-bisphosphate + 4-n-nonylphenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoform SULT1A1
-
-
?
3'-phosphoadenylylsulfate + 4-octylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-octylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-pentylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-pentylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-propylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-propylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 5alpha-androstane-3alpha,17beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 20%
-
-
r
3'-phosphoadenylylsulfate + 5alpha-androstane-3beta,17beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 83%
-
-
r
3'-phosphoadenylylsulfate + 5alpha-petromyzonol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
formation of 24-sulfate
-
-
?
3'-phosphoadenylylsulfate + 5alpha-pregnane-3beta,20alpha-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 86%
-
-
r
3'-phosphoadenylylsulfate + 5beta-androstane-3alpha,17beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 7%
-
-
r
3'-phosphoadenylylsulfate + 6-hydroxymethylbenzo[a]pyrene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation
-
-
?
3'-phosphoadenylylsulfate + 7-hydroxymethyl-12-methylbenz[a]anthracene
adenosine 3',5'-bisphosphate + 7-hydroxymethyl-12-methylbenz[a]anthracene sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 7-hydroxymethyl-12-methylbenz[a]anthracene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + aldosterone
adenosine 3',5'-bisphosphate + aldosterone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + allo-pregnanolone
adenosine 3',5'-bisphosphate + allo-pregnanolone sulfate
show the reaction diagram
-
isoenzyme 3alpha-HST
-
-
r
3'-phosphoadenylylsulfate + alpha-hydroxytamoxifen
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation to a lesser extent
-
-
?
3'-phosphoadenylylsulfate + alpha-hydroxytamoxifen
adenosine 3',5'-bisphosphate + sulfuric acid mono-[(E)-4-[4-(2-dimethylaminoethoxy)-phenyl]-3,4-diphenylbut-3-enyl] ester
show the reaction diagram
-
all enantiomers are substrates: E-(+)-alpha-hydroxytamoxifen, E-(-)-alpha-hydroxytamoxifen, Z-(+)-alpha-hydroxytamoxifen and Z-(-)-alpha-hydroxytamoxifen. The ratio of turnover-number to KM-value is higher with the Z enantiomers than with the E enantiomers
-
-
?
3'-phosphoadenylylsulfate + alpha-naphthol
adenosine 3',5'-bisphosphate + alpha-naphthyl sulfate
show the reaction diagram
-
relative activity 4%
-
-
r
3'-phosphoadenylylsulfate + amyl alcohol
adenosine 3',5'-bisphosphate + amyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androst-5-en-3beta,17alpha-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androst-5-en-3beta,17beta-diol
adenosine 3',5'-bisphosphate + androst-5-en-3beta,17beta-diol sulfate
show the reaction diagram
-
-
-
-
-
3'-phosphoadenylylsulfate + androstenediol
adenosine 3',5'-bisphosphate + androstenediol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androsterone
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + ascorbic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + benzyl alcohol
adenosine 3',5'-bisphosphate + benzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + budesonide-22R
adenosine 3',5'-bisphosphate + budesonide-22R,11 sulfate + budesonide-22R,21 sulfate
show the reaction diagram
synthetic glucocorticosteroid, used in treatment of asthma and allergic reactions, rhinitis, and inflammatory bowel disease
-
-
r
3'-phosphoadenylylsulfate + budesonide-22S
adenosine 3',5'-bisphosphate + budesonide-22S,11 sulfate + budesonide-22S,21 sulfate
show the reaction diagram
synthetic glucocorticosteroid, used in treatment of asthma and allergic reactions, rhinitis, and inflammatory bowel disease
-
-
r
3'-phosphoadenylylsulfate + chloramphenicol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cholesterol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + cis-4-methylcyclohexanol
adenosine 3',5'-bisphosphate + cis-4-methylcyclohexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + corticosterone
adenosine 3',5'-bisphosphate + corticosterone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + cortisol
adenosine 3',5'-bisphosphate + cortisol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cortisone
adenosine 3',5'-bisphosphate + cortisone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cyclohexylmethanol
adenosine 3',5'-bisphosphate + cyclohexylmethyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + D-propranolol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + daidzein
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + 1-pentyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
3'-phosphoadenylylsulfate + dopamine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoform SULT1A3
-
-
?
3'-phosphoadenylylsulfate + E-(+)-alpha-hydroxytamoxifen
adenosine 3',5'-bisphosphate + sulfuric acid mono-[(E)-4-[4-(2-dimethylaminoethoxy)-phenyl]-3,4-diphenylbut-3-enyl] ester
show the reaction diagram
-
E-(+)-alpha-hydroxytamoxifen is the only substrate, E-(-)-alpha-hydroxytamoxifen, Z-(+)-alpha-hydroxytamoxifen and Z-(-)-alpha-hydroxytamoxifen inhibit sulfation of E-(+)-alpha-hydroxytamoxifen
-
-
?
3'-phosphoadenylylsulfate + ephedrine
adenosine 3',5'-bisphosphate + ephedrine sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + epiandrosterone
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
-
3beta-hydroxy-5alpha-androstan-17-one
-
-
r
3'-phosphoadenylylsulfate + estriol
adenosine 3',5'-bisphosphate + estriol sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + estrone
adenosine 3',5'-bisphosphate + estrone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + ethanol
adenosine 3',5'-bisphosphate + ethyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + ethynylestradiol
adenosine 3',5'-bisphosphate + ethynylestradiol sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + genistein
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + hycanthone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation
-
-
?
3'-phosphoadenylylsulfate + hydrocortisone
adenosine 3',5'-bisphosphate + hydrocortisone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + isoamyl alcohol
adenosine 3',5'-bisphosphate + isoamyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + L-propranolol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + L-tyrosinemethyl ester
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 9%
-
-
r
3'-phosphoadenylylsulfate + methanol
adenosine 3',5'-bisphosphate + methyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + methyl 4-hydroxybenzoate
adenosine 3',5'-bisphosphate + methyl 4-(sulfooxy)benzoate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + minoxidil
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
antihypertensive agent and hair growth stimulator, activation by sulfation
-
-
?
3'-phosphoadenylylsulfate + N-hydroxy-2-acetylaminofluorene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation to a lesser extent
-
-
?
3'-phosphoadenylylsulfate + ouabain
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + p-nitrocatechol
adenosine 3',5'-bisphosphate + p-nitrocatechol sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + p-nitrophenol
adenosine 3',5'-bisphosphate + p-nitrophenyl sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + pregn-5-ene-3beta,20beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + R-(+)-1-naphthyl-1-ethanol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + R-(+)-2-naphthyl-1-ethanol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
both enantiomers are substrates for the enzyme
-
-
?
3'-phosphoadenylylsulfate + retinol
adenosine 3',5'-bisphosphate + retinol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + S-(-)-2-naphthyl-1-ethanol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
both enantiomers are substrates for the enzyme
-
-
?
3'-phosphoadenylylsulfate + testosterone
adenosine 3',5'-bisphosphate + testosterone 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + tetraiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
3'-phosphoadenylylsulfate + trans-4-methylcyclohexanol
adenosine 3',5'-bisphosphate + trans-4-methylcyclohexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + tridecan-1-ol
adenosine 3',5'-bisphosphate + tridecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 3,3',5'-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
best substrate
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5,5'-tetraiodothyroacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5-triiodothyroacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,5-diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3-iodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 7-oxocholesterol
adenosine 3',5'-bisphosphate + 7-oxocholesterol 3-sulfate
show the reaction diagram
O00204
SULT2B1b, SULT2B1a, and SULT2A1
-
-
?
3'-phosphoadenylyl sulfate + androsterone
adenosine 3',5'-bisphosphate + androsterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + aristolochic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O00204
herbal preparations containing Aristolochia species lead to progressive nephropathy and urothelial cancer in humans, metabolic activation of aristolichic acid, mutagenic effect, overview
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
show the reaction diagram
O00204
SULT2B1b, SULT2B1a, and SULT2A1
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
show the reaction diagram
Q29YR5
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol sulfate
show the reaction diagram
-
estrogen sulfotransferase expression in adipose tissue is regulated by testosterone, overview
-
-
?
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
show the reaction diagram
Q29YR5
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
crude enzyme activity is markedly stimulated by divalent cations like Fe2+, Co2+ and Mn2+, this effect is not observed with purified enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6-dibromophenol
-
-
2,6-Dichloro-4-nitrophenol
2,6-dichlorophenol
-
-
3'-phosphoadenylyl sulfate
-
substrate inhibition of SULT2A1, structure analysis of SULT2A1/DHEA and SULT2A1/PAP or SULT2A1/ADT, overview
3,5-dibromo-4-hydroxy-benzoic acid (6-chloro-4-oxo-4H-chromen-3-ylmethylene)-hydrazide
-
DBHM
3,5-dibromo-4-hydroxybenzoic acid (6,8-dichloro-4-oxo-4H-chromen-3-ylmethylene) hydrazide
-
DBHD
4-n-octylphenol
-
-
4-tert-octylphenol
-
-
adenosine 3',5'-bisphosphate
adenosine 5'-(beta,gamma-imido) triphosphate
-
i.e. AMP-PNP, a non-hydrolysable ATP analogue
androsterone
-
-
bis(2-ethylhexyl)phthalate
-
-
dehydroepiandrosterone
diisodecyl phthalate
-
-
E-(-)-alpha-hydroxytamoxifen
-
inhibits sulfation of E-(+)-alpha-hydroxytamoxifen
-
ioxynil
-
-
p-chloromercuribenzoate
-
-
pentabromophenol
-
-
Pentachlorophenol
-
most potent competitor
pyridoxal 5'-phosphate
-
a competitive inhibitor for sulfotransferases
S-(-)-1-naphthyl-1-ethanol
tetrabromobisphenol A
-
52% inhibition at 0.001 mM
tetrachlorobisphenol A
-
45% inhibition at 0.001 mM
tribromophenol
-
-
trichlorophenol
-
-
triiodophenol
-
-
Z-(+)-alpha-hydroxytamoxifen
-
inhibits sulfation of E-(+)-alpha-hydroxytamoxifen
-
Z-(-)-alpha-hydroxytamoxifen
-
inhibits sulfation of E-(+)-alpha-hydroxytamoxifen
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cysteine
-
essential for optimal activity
glutathione
-
activity of the purified enzyme is slightly enhanced
NaCl
-
leads to a greater activation of the cDNA-expressed DHEA ST when assayed with dehydroepiandrosterone
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
(12-methyltetraphen-7-yl)methanol
-
pH 5.5, 37C
0.96
(R)-(+)-1-naphthyl-1-ethanol
pH 7.0, 37C
0.15
(R)-(+)-1-phenyl-1-heptanol
pH 7.0, 37C
4.7
(R)-(-)-2-heptanol
-
pH 5.5, 37C
4.9
(S)-(+)-2-heptanol
-
pH 5.5, 37C
0.31
(S)-(-)-1-phenyl-1-heptanol
pH 7.0, 37C
1.7
1-amylalcohol
-
pH 5.5, 37C
3 - 106
1-butanol
0.31
1-Decanol
-
pH 5.5, 37C
0.33
1-Dodecanol
-
pH 5.5, 37C
2.2
1-Heptanol
-
pH 5.5, 37C
4.3
1-Hexanol
-
pH 5.5, 37C
26.8
1-methylcyclohexanol
-
pH 5.5, 37C
0.37
1-nonanol
-
pH 5.5, 37C
0.77
1-Octanol
-
pH 5.5, 37C
1.7 - 19.2
1-Pentanol
24 - 610
1-propanol
0.23
1-Tetradecanol
0.26
1-undecanol
-
pH 5.5, 37C
0.52
11-deoxycorticosterone
-
pH 7.5, 37C
0.000897
17-hydroxypregnenolone
-
pH 7.7, 37C
24.6
2-methyl-2-hexanol
-
pH 5.5, 37C
17
2-propanol
-
pH 7.5, 37C
0.00109
3'-phosphoadenylyl sulfate
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00013 - 0.1
3'-phosphoadenylylsulfate
0.00477
3,3',5-Triiodothyroacetic acid
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00022
3,3',5-triiodothyronine
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00295 - 0.00973
3,3'-diiodothyronine
0.00484
3,5-diiodothyronine
-
pH 6.0, 37C, isoform SULT2A1
40.3
3-ethyl-3-pentanol
-
pH 5.5, 37C
1.2
3-methyl-1-butanol
-
pH 7.5, 37C
0.00063
3beta,20beta-pregnenediol
-
pH 7.5, 37C
0.0043
4,4'-isopropylidenediphenol
pH 7.4, 37C
0.0051
4-(1,1,3,3-tetramethylbutyl)phenol
pH 7.4, 37C
0.07 - 0.16
4-butylbenzyl alcohol
0.67
4-Ethylbenzyl alcohol
-
pH 5.5, 37C
7.2
4-heptanol
-
pH 5.5, 37C
0.19
4-heptylbenzyl alcohol
-
pH 5.5, 37C
0.2
4-hexylbenzyl alcohol
-
pH 5.5, 37C
0.07 - 2.19
4-methylbenzyl alcohol
0.0715
4-n-nonylphenol
pH 7.4, 37C
0.29
4-octylbenzyl alcohol
-
pH 5.5, 37C
0.12
4-pentylbenzyl alcohol
-
pH 5.5, 37C
0.4
4-propylbenzyl alcohol
-
pH 5.5, 37C
0.008
5alpha-petromyzonol
-
-
0.72
7-hydroxymethyl-12-methylbenz[a]anthracene
pH 7.0, 37C
0.17
aldosterone
-
pH 7.5, 37C
0.000083
allopregnanolone
-
pH 7.7, 37C
0.000175 - 0.0096
androsterone
25.8
benzyl alcohol
-
pH 5.5, 37C
0.035 - 0.29
beta-estradiol
0.0209
budesonide-22R
-
pH 7.4, 37C
0.0111
budesonide-22S
-
pH 7.4, 37C
0.0017 - 0.0025
cholesterol
4.5
cis-4-methylcyclohexanol
-
pH 5.5, 37C
0.29 - 0.44
cortisol
2
cyclohexylmethanol
-
pH 5.5, 37C
0.1673
daidzein
pH 7.4, 37C
0.0006 - 0.0179
dehydroepiandrosterone
0.0465
E-(+)-alpha-hydroxytamoxifen
-
pH 8.0, 37C
0.0358
E-(-)-alpha-hydroxytamoxifen
-
pH 8.0, 37C
-
0.35
estriol
-
pH 7.5, 37C
42 - 47
ethanol
0.041
genistein
pH 7.4, 37C
1
Hexanol
-
pH 7.5, 37C
1.2
Isoamylalcohol
-
pH 5.5, 37C
3.9
minoxidil
-
pH 7.5, 37C
0.000257 - 0.0089
pregnenolone
0.24
R-(+)-1-naphthyl-1-ethanol
-
pH 7.0, 37C
0.25
R-(+)-2-naphthyl-1-ethanol
-
pH 7.0, 37C
-
0.54
S-(-)-2-naphthyl-1-ethanol
-
pH 7.0, 37C
0.07
testosterone
0.00136
tetraiodothyronine
-
pH 6.0, 37C, isoform SULT1E1
5.1
trans-4-methylcyclohexanol
-
pH 5.5, 37C
0.00463 - 0.0143
triiodothyronine
0.0669
Z-(+)-alpha-hydroxytamoxifen
-
pH 8.0, 37C
-
0.0405
Z-(-)-alpha-hydroxytamoxifen
-
pH 8.0, 37C
-
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.283
1-butanol
Rattus norvegicus
-
pH 7.5, 37C
0.417
1-Hexanol
Rattus norvegicus
-
pH 7.5, 37C
0.45
1-Pentanol
Rattus norvegicus
-
pH 7.5, 37C
0.183
1-propanol
0.217
2-propanol
Rattus norvegicus
-
pH 7.5, 37C
0.417
3-methyl-1-butanol
Rattus norvegicus
-
pH 7.5, 37C
0.133
aldosterone
Rattus norvegicus
-
pH 7.5, 37C
0.583
beta-estradiol
Rattus norvegicus
-
pH 7.5, 37C
0.183
cortisol
Rattus norvegicus
-
pH 7.5, 37C
0.15
cortisone
Rattus norvegicus
-
pH 7.5, 37C cosubstrate ethanol
2
dehydroepidandrosterone
Rattus norvegicus
-
pH 7.5, 37C
-
0.167
estriol
Rattus norvegicus
-
pH 7.5, 37C
0.0833
methanol
Rattus norvegicus
-
pH 7.5, 37C
0.467
testosterone
Rattus norvegicus
-
pH 7.5, 37C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25
(S)-(-)-1-naphthyl-1-ethanol
pH 5.5, 37C
0.0028
4-tert-octylphenol
-
-
0.003
adenosine 3',5'-bisphosphate
-
pH 8.0, 37C
0.0007 - 0.159
androsterone
0.0061 - 0.466
dehydroepiandrosterone
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00055
2,6-dibromophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00049
2,6-Dichloro-4-nitrophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00036
2,6-dichlorophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00097
ioxynil
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00016
pentabromophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00015
Pentachlorophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00097
tetrabromobisphenol A
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.0008
tribromophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00037
trichlorophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00021
triiodophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00002965
substrate 4-methylumbelliferone
0.0000577
-
partially purified enzyme
0.0000596
substrate p-nitrophenol
0.00018
-
activity with 3-keto-5alpha-petromyzonol, crude larval liver extract
0.000207
substrate p-hydroxybenzoic acid methyl ester
0.000231
-
activity with 5alpha-petromyzonol, crude larval liver extract
0.0002474
substrate p-nitrocatechol
0.0002614
substrate estriol
0.0004035
substrate ethynylestradiol
0.0004193
substrate estradiol
0.0004737
substrate estrone
0.0019
-
recombinant SULT2B1b, substrate 7beta-hydroxycholesterol
0.002038
-
partially purified enzyme
0.0048
-
recombinant SULT2B1b, substrate cholesterol 7alpha-hydroperoxide
0.0071
-
recombinant SULT2B1b, substrate 7-oxocholesterol
0.0075
-
recombinant SULT2B1b, substrate cholesterol
0.021
-
recombinant SULT2B1b, substrate 7alpha-hydroxycholesterol
0.11
-
substrate estradiol
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6.5
-
assay at
5.5
-
assay at
5.5 - 6
-
beta-estradiol and dehydroepiandrosterone as substrate
6
-
dehydroepiandrosterone as substrate
7 - 8
-
-
7 - 9
-
recombinant enzyme, expressed in Escherichia coli
7.1 - 7.5
-
assay at
7.4
-
isoform SULT2A1
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
40 - 45
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 50
-
enzyme activity at 25C and 30C drops to 35% and 56%, repective to the maximum activity at 45C, residual activity is determined to be 62% of the maximum at 50C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.12
sequence calculation, SULT2B1b
5.93
sequence calculation, SULT2B1a
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
adult, isoform SULT1A1; adult, isoform SULT1A3
Manually annotated by BRENDA team
-
epidermal keratinocyte
Manually annotated by BRENDA team
-
male white epididymal fat pad, no expression in brown fat
Manually annotated by BRENDA team
-
hepatocellular carcinoma cell line
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
prostate epithelial cells, prostate benign prostatic hyperplasia, prostate adenocarcinoma and LNCaP prostate adenocarcinoma cells
Manually annotated by BRENDA team
-
endometrial stromal, from 29 patients, reduced or no expression of EST
Manually annotated by BRENDA team
higher expression level of SULT2B1
Manually annotated by BRENDA team
-
medulloblastoma-derived cells
Manually annotated by BRENDA team
-
of male, but not of female mice. Testosterone-dependent, gender-specific expression of estrogen sulfotransferase in adipose tissue
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30200
-
SDS-PAGE
32000
-
isoenzyme 3alpha-HAST, SDS-PAGE
33000
-
isoenzyme 3beta-HAST, SDS-PAGE
33770
-
calculated from putative amino acid sequence, estimated from cDNA
35930
calculated from putative amino acid sequence, estimated from cDNA
40000
-
SDS-PAGE
50000
-
gel filtration
67200
-
gel filtration
68000
-
gel filtration
106000
-
gel filtration
135000
-
gel filtration
180000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
-
6 * 28000, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
recombinant wild-type, full-length SULT2B1b, but not truncated SULT2B1b, is phosphorylated by casein kinase or Cdc2 protein kinase in vitro
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DHEA-ST
-
human cholesterol sulfotransferase SULT2B1b
-
purified recombinant mutants M137I and M137W, hanging drop vapor diffusion method at 18C, 0.002 ml hanging drops by mixing equal volumes of protein solution and a reservoir solution containing 1.6 M (NH4)2SO4, 100 mM NaCl, and 0.1 M HEPES at pH 7.5, 3-7 days, X-ray diffraction structure determination and analysis at 2.6 and 3.0 A resolution, respectively, modeling
-
purified SULT1B1 is crystallized in the presence of 2 mM PAP using the hanging drop method at 20C by mixing for SULT1B1: 0.002 ml of the protein solution with 0.002 ml of the reservoir solution containing 0.1 M Bis-Tris, pH 6.5, 0.2 M ammonium sulfate and 16%-20% PEG 4000
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
unstable at low pH
645773
6 - 9
-
stable for 30 min at 37C
645773
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 43
42
-
30 min, the activity of the recombinant wild-type, full-length SULT2B1b is reduced by 30%, but the activity of the recombinant truncated SULT2B1bmutant is reduced 3.5fold more
45
-
90% basal activity remains after 15 min
additional information
-
the proline- and serine-rich carboxyl extension of SULT2B1b is an important site in the thermostability of this SULT isoform, overview
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
partially purified enzyme loses 30% of upon freeze-thawing, activity can be stabilized by adenosine 3',5'-bisphosphate
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, activity towards dehydroepiandrosterone as acceptor substrate is stable for several months
-
-20C, partially purified enzyme in 50% glycerol or ethylene glycol loses 30% of activity overnight
-
-80C, stable for several months
-
4C, activity declines by 60% after 4 weeks
-
4C, loses 10% activity per week upon storage
-
4C, partially purified enzyme loses 30% of activity overnight
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; recombinant GST-tagged isozymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
native and recombinant protein
native enzyme partially from brain by native PAGE
-
partial
partially, 5 ST activities in liver preparation
-
recombinant GST-tagged SULT2B1 by glutathione affinity chromatography
-
recombinant His-tagged full-length SULT2B1b and of truncated SULT2B1b lacking proline- and serine-rich carboxyl extension from Escherichia coli strain DH5alpha by nickel affinity chromatography
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recombinant SULT2A1 from Escherichia coli by anion exchange chromatography, ultrafiltration, and hydroxyapatite chromatography
-
sulfotransferase 1
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; cloned and expressed in Escherichia coli
-
; gene SULT2B1, isozymes SULT2B1a and SULT2B1b as a result of an alternative exon I, DNA and amino acid sequence determination and analysis, genetic structure, expression analysis, comparison of sequence and genetic structure, overview, overexpression of GST-tagged isozymes in Escherichia coli strain BL21(DE3)
; SULT2A1 bacterially expressed as His tagged protein in Escherichia coli
cDNA encoding alcohol/hydroxysteroid sulfotransferase h-ST-a cloned from a liver cDNA library
-
cDNA encoding liver DHEA-ST isolated from Uni-Zap XR human liver cDNA library cloned, expressed in green monkey COS-7 cells
-
cDNA of placental EST cloned, COS-7 cells transfected with mpEST
cloned and expressed in Escherichia coli
-
cloning of AST-RB2 (ST2A8) from cDNA library, expressed in Escherichia coli
-
DHEA ST and SULT2A1 cloned and expressed in COS-1 cells
-
DHEA ST cloned from cDNA and expressed in COS-1 cells
-
DHEA-ST cloned and expressed in glutathione sulfotransferase fusion form in Escherichia coli
-
DHEA-ST cloned and expressed in Salmonella typhimurium, Escherichia coli and COS cells
-
expression analysis in brain enzyme extracts
-
expression in HepG2 cells or CaCo-2 cells
-
expression of GFP-tagged wild-type allozymes and mutant truncated SULT2B1a and SULT2B1b variants lacking the SULT2B1 proline-rich tail in COS-1 cells; isozymes SULT2B1a and SULT2B1b are encoded by a single SULT2B1 gene as a result of alternative transcription initiation and alternative splicing, genotyping of African-American and Caucasian-American individuals, transient expression of GFP-tagged SULT2B1a and SULT2B1b in COS-1 cells, in vitro transcription and translation of truncated SULT2B1a and SULT2B1b variants that lack the SULT2B1 proline-rich tail using rabbit reticulocyte lysate
-
expression of GST-tagged SULT2B1; expression of SULT2B1b eliminates the cytotoxic effect of 7-oxo-cholesterol on 293T cells, which are normally devoid of SULT2B1b
-
expression of His-tagged full-length SULT2B1b and of truncated SULT2B1b lacking proline- and serine-rich carboxyl extension in Escherichia coli strain DH5alpha, expression and serine phosphorylation of SULT2B1b in transfected BeWo cells, overview
-
expression of SULTs in Escherichia coli strain BL21 DE3, in Salmonella typhimurium strain TA1538, and in chinese hamster V79 cells, and application of aristolochic acid, mutagenicity with expression of different SULT isozymes and allozymes of SULT1A1, detailed overview
presence of seven SULT2A genes, including SULT2A1, within a cluster on chromosome 7, DNA and amino acid sequence determination and analysis, age- and sex-dependent expressions of the seven genes, overview
-
STa cloned into pET-3c vector and expressed in Escherichia coli BL21
SULT2A1 real time RT-PCR expression analysis in presence of different plasticizers
-
SULT2A1, expression in Caco-2 cells and analysis of the transcriptional regulation mechanism of hSULT2A1 promoter, quantitative enzyme expression analysis. Constitutive androstane receptor and retinoid X receptor alpha are involved in the transcriptional regulation of hSULT2A1, overview
-
SULT2A1, expression in Escherichia coli
-
SULT2B1a and SULT2B1b, DNA and amino acid sequence determination and analysis, genetic structure
SULT2B1a and SULT2B1b, DNA and amino acid sequence determination and analysis, genetic structure, the two different isozymes are encoded by the gene SULT2B1 utilizing different start sites of transcription resulting in the incorporation of different first exons, SULT2B1a and SULT2B1b are 350 and 365 amino acids in length, respectively, and the last 342 amino acids are identical, expression of His-tagged or GST-tagged SULT2B1b in Escherichia coli, expression of SULT2B1a and SULT2B1b in COS-1 cells
-
transient expression of SULT2A1 in Hep-G2 cells, expression analysis in presence or absence of rifampicin and/or PXR, regulation, overview
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
iodothyronine sulfating activity in the liver cytosol is increased in tadpoles at premetamorphic (stages IX-X) and metamorphic climax (stage XX) stages, and in adult frogs
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D176N
-
naturally occuring polymorphism in SULT2B1a
L36S
-
naturally occuring polymorphism in SULT2B1a
M137I
-
site-directed mutagenesis, substrate binding structure, the Ki for androsterone is increased 28.6fold compared to the wild-type enzyme, overview
M137K
-
site-directed mutagenesis, inactive mutant
M137K/Y238A
-
site-directed mutagenesis, inactive mutant
M137V
-
site-directed mutagenesis, substrate binding structure, the Ki for androsterone is increased 5fold and for dehydroepiandrosterone 9fold compared to the wild-type enzyme, overview
M137V/Y238A
-
site-directed mutagenesis, the mutant shows no substrate inhibition by dehydroepiandrosterone
M137W
-
site-directed mutagenesis, substrate binding structure, the Ki for androsterone is increased 11.1fold compared to the wild-type enzyme, overview
M137W/Y238A
-
site-directed mutagenesis, the mutant shows no substrate inhibition by dehydroepiandrosterone
P330L
-
naturally occuring polymorphism in SULT2B1a
R215H
-
naturally occuring polymorphism in SULT2B1a
V260A
-
site-directed mutagenesis, a monomeric mutant
V260A/Y238A
-
site-directed mutagenesis, a monomeric mutant, shows increased Ki for dehydroepiandosterone compared to the wild-type enzyme, no inhibition by androsterone
Y238A
-
site-directed mutagenesis, exhibits no substrate inhibition with ADT as substrate, the Ki value of DHEA increases 7.4fold compared with that of wild-type SULT2A1, no inhibition by androsterone
Y238F
-
site-directed mutagenesis, the Ki values of DHEA and ADT for Y238F SULT2A1 mutant increase for approximately 2 to 3fold compared with those of the wild type enzyme
Y238W
-
site-directed mutagenesis, the Ki values of DHEA and ADT for Y238W SULT2A1 mutant increase for approximately 2 to 3fold compared with those of the wild type enzyme
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
-
EST expression is a prognostic factor in breast cancer
medicine