Information on EC 2.8.2.2 - alcohol sulfotransferase

Word Map on EC 2.8.2.2
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.8.2.2
-
RECOMMENDED NAME
GeneOntology No.
alcohol sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + an alcohol = adenosine 3',5'-bisphosphate + an alkyl sulfate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfate group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Steroid hormone biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:alcohol sulfotransferase
Primary and secondary alcohols, including aliphatic alcohols, ascorbic acid, chloramphenicol, ephedrine and hydroxysteroids, but not phenolic steroids, can act as acceptors (cf. EC 2.8.2.15 steroid sulfotransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
9032-76-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
guinea pig
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
hamster
-
-
Manually annotated by BRENDA team
rabbit
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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knock-down of SULT2B1b inhibits cell growth and cyclinB1 levels in human hepatocarcinoma cells and suppressed xenograft growth in vivo
metabolism
-
isoform SULT2B1b and its enzymatic by-product cholesterol sulfate are important metabolic regulators that control glucose metabolism. Mechanistically, cholesterol sulfate and SULT2B1b inhibit gluconeogenesis by suppressing the expression of acetyl coenzyme A synthetase, leading to decreased acetylation and nuclear exclusion of hepatocyte nuclear factor 4alpha.
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
-
SULT1B1
-
-
?
3'-phosphoadenylyl sulfate + 17alpha-hydroxypregnenolone
adenosine 3',5'-bisphosphate + 17alpha-hydroxypregnenolone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5'-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
best substrate
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5,5'-tetraiodothyroacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5-triiodothyroacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,5-diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3-iodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 5alpha,6alpha-epoxycholesterol
adenosine 3',5'-bisphosphate + 5alpha,6alpha-epoxycholesteryl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 5beta,6beta-epoxycholesterol
adenosine 3',5'-bisphosphate + 5beta,6beta-epoxycholesteryl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 7-oxo-cholesterol
adenosine 3',5'-bisphosphate + 7-oxo-cholesteryl 3-sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 7-oxocholesterol
adenosine 3',5'-bisphosphate + 7-oxocholesterol 3-sulfate
show the reaction diagram
SULT2B1b, SULT2B1a, and SULT2A1
-
-
?
3'-phosphoadenylyl sulfate + 7alpha-hydroxycholesterol
adenosine 3',5'-bisphosphate + 7alpha-hydroxycholesterol 3-sulfate
show the reaction diagram
SULT2B1b, the 7alpha derivative is highly preferred
-
-
?
3'-phosphoadenylyl sulfate + 7alpha-hydroxycholesterol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + 7beta-hydroxycholesterol
adenosine 3',5'-bisphosphate + 7beta-hydroxycholesterol 3-sulfate
show the reaction diagram
SULT2B1b, the 7alpha derivative is highly preferred
-
-
?
3'-phosphoadenylyl sulfate + 7beta-hydroxycholesterol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylyl sulfate + an alcohol
adenosine 3',5'-bisphosphate + an alkyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + androsterone
adenosine 3',5'-bisphosphate + androsterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + aristolochic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + cholesterol 7alpha-hydroperoxide
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
SULT2B1b
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + genistein
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + raloxifene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + tibolone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + (R)-(-)-2-heptanol
adenosine 3',5'-bisphosphate + (R)-(-)-2-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + (S)-(+)-2-heptanol
adenosine 3',5'-bisphosphate + (S)-(+)-2-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + (S)-(-)-1-phenyl-1-heptanol
adenosine 3',5'-bisphosphate + (S)-(-)-1-phenyl-1-heptyl sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + 1'-hydroxysafrole
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation to a lesser extent
-
-
?
3'-phosphoadenylylsulfate + 1,10-phenanthroline
adenosine 3',5'-bisphosphate + 1,10-phenanthroline sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 1-butanol
adenosine 3',5'-bisphosphate + 1-butanoyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 1-decanol
adenosine 3',5'-bisphosphate + 1-decyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-dodecanol
adenosine 3',5'-bisphosphate + 1-dodecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-heptanol
adenosine 3',5'-bisphosphate + 1-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-hexanol
adenosine 3',5'-bisphosphate + 1-hexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-hydroxymethylpyrene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation
-
-
?
3'-phosphoadenylylsulfate + 1-hydroxypyrene
adenosine 3',5'-bisphosphate + 1-hydroxypyrene sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 1-methylcyclohexanol
adenosine 3',5'-bisphosphate + 1-methylcyclohexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 1-nonanol
adenosine 3',5'-bisphosphate + 1-nonyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-octanol
adenosine 3',5'-bisphosphate + 1-octyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-pentanol
adenosine 3',5'-bisphosphate + 1-pentyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-propanol
adenosine 3',5'-bisphosphate + 1-propyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-pyrenemethanol
adenosine 3',5'-bisphosphate + 1-pyrenemethylsulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 1-tetradecanol
adenosine 3',5'-bisphosphate + 1-tetradecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-undecanol
adenosine 3',5'-bisphosphate + 1-undecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 11-deoxycorticosterone
adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + 17-hydroxypregnenolone
adenosine 3',5'-bisphosphate + pregn-4-en-3,20-dione 3-sulfate
show the reaction diagram
-
3beta,17alpha-dihydroxypregn-4-en-3,20-dione, isoenzyme 3beta-HST
-
-
r
3'-phosphoadenylylsulfate + 17alpha-ethinylestradiol
adenosine 3',5'-bisphosphate + 17alpha-ethinylestradiol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
adenosine 3',5'-bisphosphate + 2'-fluoro-4-hydroxy-3,5-dichlorobiphenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 2-aminofluorene
adenosine 3',5'-bisphosphate + 2-aminofluorene sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 2-aminophenol
adenosine 3',5'-bisphosphate + 2-aminophenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 2-ethylphenol
adenosine 3',5'-bisphosphate + 2-ethylphenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 2-fluoro-4-hydroxy-3,5-dichlorobiphenyl
adenosine 3',5'-bisphosphate + 2-fluoro-4-hydroxy-3,5-dichlorobiphenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 2-hydroxy-catecholestrogen
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
best substrate for this enzyme, also catalyzes the transformation of 4-hydroxy-estrogens and 16-hydroxy-estrogens, but with a lower affinity
-
-
r
3'-phosphoadenylylsulfate + 2-hydroxyestradiol
adenosine 3',5'-bisphosphate + 2-hydroxyestradiol sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 2-methyl-2-hexanol
adenosine 3',5'-bisphosphate + 1,1-dimethylpentyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-naphthol
adenosine 3',5'-bisphosphate + 2-naphthyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 2-nitrophenol
adenosine 3',5'-bisphosphate + 2-nitrophenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 2-propanol
adenosine 3',5'-bisphosphate + 2-propyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 22-hydroxycholesterol
adenosine 3',5'-bisphosphate + 22-hydroxycholesterol sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 24,25-epoxycholesterol
adenosine 3',5'-bisphosphate + 24,25-epoxycholesterol sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 25-hydroxycholesterol
adenosine 3',5'-bisphosphate + 25-hydroxycholesterol sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 3'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
adenosine 3',5'-bisphosphate + 3'-fluoro-4-hydroxy-3,5-dichlorobiphenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 3,3'-diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
3'-phosphoadenylylsulfate + 3,5-diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
3'-phosphoadenylylsulfate + 3-aminophenol
adenosine 3',5'-bisphosphate + 3-aminophenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 3-ethyl-3-pentanol
adenosine 3',5'-bisphosphate + 3-ethyl-3-pentyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3-ethylphenol
adenosine 3',5'-bisphosphate + 3-ethylphenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 3-keto-5alpha-petromyzonol
adenosine 3',5'-bisphosphate + 7alpha,12alpha,24-trihydroxy-5alpha-cholan-3-one 24 sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 3-methyl-1-butanol
adenosine 3',5'-bisphosphate + 3-methyl-1-butyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3-nitrophenol
adenosine 3',5'-bisphosphate + 3-nitrophenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 3-O-phenyl-17beta-estradiol
adenosine 3',5'-bisphosphate + 3-phenolic hydroxyl beta-estradiol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3alpha-hydroxy-5beta-androstan-17-one
adenosine 3',5'-bisphosphate + 5beta-androstan-17-one 3-sulfate
show the reaction diagram
-
relative activity 5%
-
-
?
3'-phosphoadenylylsulfate + 3beta,17alpha-dihydroxy-pregn-5-ene-20-one
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 4%
-
-
r
3'-phosphoadenylylsulfate + 3beta-hydroxy-5beta-androstan-17-one
adenosine 3',5'-bisphosphate + 5beta-androstan-17-one 3-sulfate
show the reaction diagram
-
relative activity 45%
-
-
r
3'-phosphoadenylylsulfate + 4'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
adenosine 3',5'-bisphosphate + 4'-fluoro-4-hydroxy-3,5-dichlorobiphenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4,4'-isopropylidenediphenol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 4-(1,1,3,3-tetramethylbutyl)phenol
adenosine 3',5'-bisphosphate + 4-(1,1,3,3-tetramethylbutyl)phenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 4-aminophenol
adenosine 3',5'-bisphosphate + 4-aminophenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 4-butylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-butylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-ethylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-ethylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-ethylphenol
adenosine 3',5'-bisphosphate + 4-ethylphenyl sulfate
show the reaction diagram
best substrate
-
-
?
3'-phosphoadenylylsulfate + 4-heptanol
adenosine 3',5'-bisphosphate + 4-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-heptylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-heptylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-hexylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-hexylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-hydroxy-3,5-dichlorobiphenyl
adenosine 3',5'-bisphosphate + 4-hydroxy-3,5-dichlorobiphenyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 4-methylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-methylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-methylumbelliferone
adenosine 3',5'-bisphosphate + 4-methylumbelliferone sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + 4-n-nonylphenol
adenosine 3',5'-bisphosphate + 4-n-nonylphenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + 4-nitrophenyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoform SULT1A1
-
-
?
3'-phosphoadenylylsulfate + 4-octylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-octylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-pentylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-pentylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-propylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-propylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 5-sulfosalicylic acid
adenosine 3',5'-bisphosphate + 5-sulfosalicyl sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 5alpha-androstane-3alpha,17beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 20%
-
-
r
3'-phosphoadenylylsulfate + 5alpha-androstane-3beta,17beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 83%
-
-
r
3'-phosphoadenylylsulfate + 5alpha-petromyzonol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
formation of 24-sulfate
-
-
?
3'-phosphoadenylylsulfate + 5alpha-pregnane-3beta,20alpha-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 86%
-
-
r
3'-phosphoadenylylsulfate + 5beta-androstane-3alpha,17beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 7%
-
-
r
3'-phosphoadenylylsulfate + 6-hydroxymethylbenzo[a]pyrene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation
-
-
?
3'-phosphoadenylylsulfate + 7-hydroxymethyl-12-methylbenz[a]anthracene
adenosine 3',5'-bisphosphate + 7-hydroxymethyl-12-methylbenz[a]anthracene sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + 7-hydroxymethyl-12-methylbenz[a]anthracene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + aldosterone
adenosine 3',5'-bisphosphate + aldosterone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + allo-pregnanolone
adenosine 3',5'-bisphosphate + allo-pregnanolone sulfate
show the reaction diagram
-
isoenzyme 3alpha-HST
-
-
r
3'-phosphoadenylylsulfate + alpha-hydroxytamoxifen
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation to a lesser extent
-
-
?
3'-phosphoadenylylsulfate + alpha-hydroxytamoxifen
adenosine 3',5'-bisphosphate + sulfuric acid mono-[(E)-4-[4-(2-dimethylaminoethoxy)-phenyl]-3,4-diphenylbut-3-enyl] ester
show the reaction diagram
-
all enantiomers are substrates: E-(+)-alpha-hydroxytamoxifen, E-(-)-alpha-hydroxytamoxifen, Z-(+)-alpha-hydroxytamoxifen and Z-(-)-alpha-hydroxytamoxifen. The ratio of turnover-number to KM-value is higher with the Z enantiomers than with the E enantiomers
-
-
?
3'-phosphoadenylylsulfate + alpha-naphthol
adenosine 3',5'-bisphosphate + alpha-naphthyl sulfate
show the reaction diagram
-
relative activity 4%
-
-
r
3'-phosphoadenylylsulfate + amyl alcohol
adenosine 3',5'-bisphosphate + amyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androst-5-en-3beta,17alpha-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androst-5-en-3beta,17beta-diol
adenosine 3',5'-bisphosphate + androst-5-en-3beta,17beta-diol sulfate
show the reaction diagram
-
-
-
-
-
3'-phosphoadenylylsulfate + androstenediol
adenosine 3',5'-bisphosphate + androstenediol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androsterone
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + ascorbic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + benzyl alcohol
adenosine 3',5'-bisphosphate + benzyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + budesonide-22R
adenosine 3',5'-bisphosphate + budesonide-22R,11 sulfate + budesonide-22R,21 sulfate
show the reaction diagram
synthetic glucocorticosteroid, used in treatment of asthma and allergic reactions, rhinitis, and inflammatory bowel disease
-
-
r
3'-phosphoadenylylsulfate + budesonide-22S
adenosine 3',5'-bisphosphate + budesonide-22S,11 sulfate + budesonide-22S,21 sulfate
show the reaction diagram
synthetic glucocorticosteroid, used in treatment of asthma and allergic reactions, rhinitis, and inflammatory bowel disease
-
-
r
3'-phosphoadenylylsulfate + chloramphenicol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cholesterol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + cis-4-methylcyclohexanol
adenosine 3',5'-bisphosphate + cis-4-methylcyclohexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + corticosterone
adenosine 3',5'-bisphosphate + corticosterone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + cortisol
adenosine 3',5'-bisphosphate + cortisol sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + cortisone
adenosine 3',5'-bisphosphate + cortisone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cycloheximide
adenosine 3',5'-bisphosphate + cycloheximide sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + cyclohexylmethanol
adenosine 3',5'-bisphosphate + cyclohexylmethyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + D-propranolol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + daidzein
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + 1-pentyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate
show the reaction diagram
-
-
-
-
-
3'-phosphoadenylylsulfate + diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
3'-phosphoadenylylsulfate + dopamine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoform SULT1A3
-
-
?
3'-phosphoadenylylsulfate + E-(+)-alpha-hydroxytamoxifen
adenosine 3',5'-bisphosphate + sulfuric acid mono-[(E)-4-[4-(2-dimethylaminoethoxy)-phenyl]-3,4-diphenylbut-3-enyl] ester
show the reaction diagram
-
E-(+)-alpha-hydroxytamoxifen is the only substrate, E-(-)-alpha-hydroxytamoxifen, Z-(+)-alpha-hydroxytamoxifen and Z-(-)-alpha-hydroxytamoxifen inhibit sulfation of E-(+)-alpha-hydroxytamoxifen
-
-
?
3'-phosphoadenylylsulfate + ephedrine
adenosine 3',5'-bisphosphate + ephedrine sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + epiandrosterone
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
-
3beta-hydroxy-5alpha-androstan-17-one
-
-
r
3'-phosphoadenylylsulfate + estriol
adenosine 3',5'-bisphosphate + estriol sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + estrone
adenosine 3',5'-bisphosphate + estrone sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + ethanol
adenosine 3',5'-bisphosphate + ethyl sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + ethynylestradiol
adenosine 3',5'-bisphosphate + ethynylestradiol sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + genistein
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + hycanthone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation
-
-
?
3'-phosphoadenylylsulfate + hycanthone
adenosine 3',5'-bisphosphate + hycanthone sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + hydrocortisone
adenosine 3',5'-bisphosphate + hydrocortisone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + hydroquinone
adenosine 3',5'-bisphosphate + hydroquinone sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + isoamyl alcohol
adenosine 3',5'-bisphosphate + isoamyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + L-propranolol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + L-tyrosinemethyl ester
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 9%
-
-
r
3'-phosphoadenylylsulfate + m-cresol
adenosine 3',5'-bisphosphate + m-cresol sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + methanol
adenosine 3',5'-bisphosphate + methyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + methyl 4-hydroxybenzoate
adenosine 3',5'-bisphosphate + methyl 4-(sulfooxy)benzoate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + minoxidil
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
antihypertensive agent and hair growth stimulator, activation by sulfation
-
-
?
3'-phosphoadenylylsulfate + N-hydroxy-2-acetylaminofluorene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation to a lesser extent
-
-
?
3'-phosphoadenylylsulfate + o-cresol
adenosine 3',5'-bisphosphate + o-cresol sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + ouabain
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + p-cresol
adenosine 3',5'-bisphosphate + p-cresol sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + p-nitrocatechol
adenosine 3',5'-bisphosphate + p-nitrocatechol sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + p-nitrophenol
adenosine 3',5'-bisphosphate + p-nitrophenyl sulfate
show the reaction diagram
-
-
-
r
3'-phosphoadenylylsulfate + pregn-5-ene-3beta,20beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + quercetin dehydrate
adenosine 3',5'-bisphosphate + quercetin dehydrate sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + R-(+)-1-naphthyl-1-ethanol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
3'-phosphoadenylylsulfate + R-(+)-2-naphthyl-1-ethanol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
both enantiomers are substrates for the enzyme
-
-
?
3'-phosphoadenylylsulfate + retinol
adenosine 3',5'-bisphosphate + retinol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + S-(-)-2-naphthyl-1-ethanol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
both enantiomers are substrates for the enzyme
-
-
?
3'-phosphoadenylylsulfate + sodium 4-amino-salicylate
adenosine 3',5'-bisphosphate + sodium 4-amino-salicylate sulfate
show the reaction diagram
-
-
-
?
3'-phosphoadenylylsulfate + testosterone
adenosine 3',5'-bisphosphate + testosterone 3-sulfate
show the reaction diagram
3'-phosphoadenylylsulfate + tetraiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
3'-phosphoadenylylsulfate + trans-4-methylcyclohexanol
adenosine 3',5'-bisphosphate + trans-4-methylcyclohexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + tridecan-1-ol
adenosine 3',5'-bisphosphate + tridecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 17alpha-hydroxypregnenolone
adenosine 3',5'-bisphosphate + 17alpha-hydroxypregnenolone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5'-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
best substrate
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5,5'-tetraiodothyroacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5-triiodothyroacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,5-diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3-iodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 7-oxocholesterol
adenosine 3',5'-bisphosphate + 7-oxocholesterol 3-sulfate
show the reaction diagram
O00204
SULT2B1b, SULT2B1a, and SULT2A1
-
-
?
3'-phosphoadenylyl sulfate + an alcohol
adenosine 3',5'-bisphosphate + an alkyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + androsterone
adenosine 3',5'-bisphosphate + androsterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + aristolochic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O00204
herbal preparations containing Aristolochia species lead to progressive nephropathy and urothelial cancer in humans, metabolic activation of aristolichic acid, mutagenic effect, overview
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
show the reaction diagram
O00204
SULT2B1b, SULT2B1a, and SULT2A1
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
show the reaction diagram
Q29YR5
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol sulfate
show the reaction diagram
-
estrogen sulfotransferase expression in adipose tissue is regulated by testosterone, overview
-
-
?
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
show the reaction diagram
Q29YR5
-
-
-
?
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
crude enzyme activity is markedly stimulated by divalent cations like Fe2+, Co2+ and Mn2+, this effect is not observed with purified enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
-
-
-
2,6-dibromophenol
-
-
2,6-Dichloro-4-nitrophenol
2,6-dichlorophenol
-
-
2-fluoro-4-hydroxy-3,5-dichlorobiphenyl
-
-
-
3'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
-
-
-
3'-phosphoadenylyl sulfate
-
substrate inhibition of SULT2A1, structure analysis of SULT2A1/DHEA and SULT2A1/PAP or SULT2A1/ADT, overview
3,3',4'-dihydroxy-4-monochlorobiphenyl
-
-
-
3,5-dibromo-4-hydroxy-benzoic acid (6-chloro-4-oxo-4H-chromen-3-ylmethylene)-hydrazide
-
DBHM
3,5-dibromo-4-hydroxybenzoic acid (6,8-dichloro-4-oxo-4H-chromen-3-ylmethylene) hydrazide
-
DBHD
4'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
-
-
-
4'-hydroxy-2',3,4-trichlorobiphenyl
-
-
4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl
-
-
4'-hydroxy-2,3',4-trichlorobiphenyl
-
-
4'-hydroxy-2,3'-dichlorobiphenyl
-
-
4'-hydroxy-2,5-dichlorobiphenyl
-
-
4'-hydroxy-3,4-dichlorobiphenyl
-
-
4'-hydroxy-4-monochlorobiphenyl
-
-
4-hydroxy-2',3,5-trichlorobiphenyl
-
-
4-hydroxy-2,4'-dichlorobiphenyl
-
-
4-hydroxy-3,3',5-trichlorobiphenyl
-
-
4-hydroxy-3,3'-dichlorobiphenyl
-
-
4-hydroxy-3,5-dichlorobiphenyl
-
-
4-n-octylphenol
-
-
4-tert-octylphenol
-
-
5,3',4'-dihydroxy-2,3-dichlorobiphenyl
-
-
-
6'-hydroxy-3,3'4-trichlorobiphenyl
-
-
-
adenosine 3',5'-bisphosphate
adenosine 5'-(beta,gamma-imido) triphosphate
-
i.e. AMP-PNP, a non-hydrolysable ATP analogue
androsterone
-
-
bis(2-ethylhexyl)phthalate
-
-
dehydroepiandrosterone
diisodecyl phthalate
-
-
E-(-)-alpha-hydroxytamoxifen
-
inhibits sulfation of E-(+)-alpha-hydroxytamoxifen
ioxynil
-
-
p-chloromercuribenzoate
-
-
pentabromophenol
-
-
Pentachlorophenol
-
most potent competitor
pyridoxal 5'-phosphate
-
a competitive inhibitor for sulfotransferases
S-(-)-1-naphthyl-1-ethanol
tetrabromobisphenol A
-
52% inhibition at 0.001 mM
tetrachlorobisphenol A
-
45% inhibition at 0.001 mM
tribromophenol
-
-
trichlorophenol
-
-
triiodophenol
-
-
Z-(+)-alpha-hydroxytamoxifen
-
inhibits sulfation of E-(+)-alpha-hydroxytamoxifen
-
Z-(-)-alpha-hydroxytamoxifen
-
inhibits sulfation of E-(+)-alpha-hydroxytamoxifen
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cysteine
-
essential for optimal activity
glutathione
-
activity of the purified enzyme is slightly enhanced
NaCl
-
leads to a greater activation of the cDNA-expressed DHEA ST when assayed with dehydroepiandrosterone
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
(12-methyltetraphen-7-yl)methanol
-
pH 5.5, 37C
0.96
(R)-(+)-1-naphthyl-1-ethanol
pH 7.0, 37C
0.15
(R)-(+)-1-phenyl-1-heptanol
pH 7.0, 37C
4.7
(R)-(-)-2-heptanol
-
pH 5.5, 37C
4.9
(S)-(+)-2-heptanol
-
pH 5.5, 37C
0.31
(S)-(-)-1-phenyl-1-heptanol
pH 7.0, 37C
1.7
1-amylalcohol
-
pH 5.5, 37C
3 - 106
1-butanol
0.31
1-Decanol
-
pH 5.5, 37C
0.33
1-Dodecanol
-
pH 5.5, 37C
2.2
1-Heptanol
-
pH 5.5, 37C
4.3
1-Hexanol
-
pH 5.5, 37C
26.8
1-methylcyclohexanol
-
pH 5.5, 37C
0.37
1-nonanol
-
pH 5.5, 37C
0.77
1-Octanol
-
pH 5.5, 37C
1.7 - 19.2
1-Pentanol
24 - 610
1-propanol
0.23
1-Tetradecanol
-
pH 5.5, 37C
0.23
1-tridecanol
-
pH 5.5, 37C
0.26
1-undecanol
-
pH 5.5, 37C
0.52
11-deoxycorticosterone
-
pH 7.5, 37C
0.000897
17-hydroxypregnenolone
-
pH 7.7, 37C
0.0037
2'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
-
at pH 7.0 and 37C
-
1.16
2-Ethylphenol
at pH 7.5 and 30C
0.0256
2-fluoro-4-hydroxy-3,5-dichlorobiphenyl
-
at pH 7.0 and 37C
-
24.6
2-methyl-2-hexanol
-
pH 5.5, 37C
0.312
2-Nitrophenol
at pH 7.5 and 30C
17
2-propanol
-
pH 7.5, 37C
0.0063
3'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
-
at pH 7.0 and 37C
-
0.00109
3'-phosphoadenylyl sulfate
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00013 - 0.1
3'-phosphoadenylylsulfate
0.00477
3,3',5-Triiodothyroacetic acid
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00022
3,3',5-triiodothyronine
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00295 - 0.00973
3,3'-diiodothyronine
0.00484
3,5-diiodothyronine
-
pH 6.0, 37C, isoform SULT2A1
40.3
3-ethyl-3-pentanol
-
pH 5.5, 37C
0.152
3-Ethylphenol
at pH 7.5 and 30C
1.2
3-methyl-1-butanol
-
pH 7.5, 37C
0.475
3-Nitrophenol
at pH 7.5 and 30C
0.00063
3beta,20beta-pregnenediol
-
pH 7.5, 37C
0.0121
4'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
-
at pH 7.0 and 37C
-
0.0043
4,4'-isopropylidenediphenol
pH 7.4, 37C
0.0051
4-(1,1,3,3-tetramethylbutyl)phenol
pH 7.4, 37C
0.07 - 0.16
4-butylbenzyl alcohol
0.67
4-Ethylbenzyl alcohol
-
pH 5.5, 37C
0.135
4-Ethylphenol
at pH 7.5 and 30C
7.2
4-heptanol
-
pH 5.5, 37C
0.19
4-heptylbenzyl alcohol
-
pH 5.5, 37C
0.2
4-hexylbenzyl alcohol
-
pH 5.5, 37C
0.0085
4-hydroxy-3,5-dichlorobiphenyl
-
at pH 7.0 and 37C
0.07 - 2.19
4-methylbenzyl alcohol
0.0715
4-n-nonylphenol
pH 7.4, 37C
0.276
4-nitrophenol
at pH 7.5 and 30C
0.29
4-octylbenzyl alcohol
-
pH 5.5, 37C
0.12
4-pentylbenzyl alcohol
-
pH 5.5, 37C
0.4
4-propylbenzyl alcohol
-
pH 5.5, 37C
0.008
5alpha-petromyzonol
-
-
0.72
7-hydroxymethyl-12-methylbenz[a]anthracene
pH 7.0, 37C
0.17
aldosterone
-
pH 7.5, 37C
0.000083
allopregnanolone
-
pH 7.7, 37C
0.000175 - 0.0096
androsterone
0.0685 - 25.8
benzyl alcohol
0.035 - 0.29
beta-estradiol
0.0209
budesonide-22R
-
pH 7.4, 37C
0.0111
budesonide-22S
-
pH 7.4, 37C
0.0017 - 0.0025
cholesterol
4.5
cis-4-methylcyclohexanol
-
pH 5.5, 37C
0.29 - 0.44
cortisol
0.01
cycloheximide
at pH 7.5 and 30C
2
cyclohexylmethanol
-
pH 5.5, 37C
0.1673
daidzein
pH 7.4, 37C
0.0006 - 0.0179
dehydroepiandrosterone
0.0465
E-(+)-alpha-hydroxytamoxifen
-
pH 8.0, 37C
0.0358
E-(-)-alpha-hydroxytamoxifen
-
pH 8.0, 37C
0.35
estriol
-
pH 7.5, 37C
42 - 47
ethanol
0.041
genistein
pH 7.4, 37C
1
Hexanol
-
pH 7.5, 37C
1.2
Isoamylalcohol
-
pH 5.5, 37C
0.18
m-cresol
at pH 7.5 and 30C
3.9
minoxidil
-
pH 7.5, 37C
0.035
o-Cresol
at pH 7.5 and 30C
0.494
p-Cresol
at pH 7.5 and 30C
0.000257 - 0.0089
pregnenolone
0.24
R-(+)-1-naphthyl-1-ethanol
-
pH 7.0, 37C
0.25
R-(+)-2-naphthyl-1-ethanol
-
pH 7.0, 37C
-
0.54
S-(-)-2-naphthyl-1-ethanol
-
pH 7.0, 37C
0.07
testosterone
0.00136
tetraiodothyronine
-
pH 6.0, 37C, isoform SULT1E1
5.1
trans-4-methylcyclohexanol
-
pH 5.5, 37C
0.00463 - 0.0143
triiodothyronine
0.0669
Z-(+)-alpha-hydroxytamoxifen
-
pH 8.0, 37C
-
0.0405
Z-(-)-alpha-hydroxytamoxifen
-
pH 8.0, 37C
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.283
1-butanol
Rattus norvegicus
-
pH 7.5, 37C
0.417
1-Hexanol
Rattus norvegicus
-
pH 7.5, 37C
0.45
1-Pentanol
Rattus norvegicus
-
pH 7.5, 37C
0.183
1-propanol
Rattus norvegicus
-
pH 7.5, 37C
0.183
11-deoxycorticosterone
Rattus norvegicus
-
pH 7.5, 37C
0.000112
2-Ethylphenol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
0.000459
2-Nitrophenol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
0.217
2-propanol
Rattus norvegicus
-
pH 7.5, 37C
0.000053
3-Ethylphenol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
0.417
3-methyl-1-butanol
Rattus norvegicus
-
pH 7.5, 37C
0.000389
3-Nitrophenol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
0.000109
4-Ethylphenol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
0.000545
4-nitrophenol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
0.133
aldosterone
Rattus norvegicus
-
pH 7.5, 37C
0.013
benzyl alcohol
Homo sapiens
O43704, P50225, P50226
isoform SULT1A1, at pH 7.0 and 37C; isoform SULT1A1, at pH 7.0 and 37C
0.583
beta-estradiol
Rattus norvegicus
-
pH 7.5, 37C
0.183
cortisol
Rattus norvegicus
-
pH 7.5, 37C
0.15
cortisone
Rattus norvegicus
-
pH 7.5, 37C cosubstrate ethanol
2
dehydroepidandrosterone
Rattus norvegicus
-
pH 7.5, 37C
-
0.167
estriol
Rattus norvegicus
-
pH 7.5, 37C
0.00004
m-cresol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
0.0833
methanol
Rattus norvegicus
-
pH 7.5, 37C
0.000035
o-Cresol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
0.000128
p-Cresol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
0.467
testosterone
Rattus norvegicus
-
pH 7.5, 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10.2
2'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
Homo sapiens
-
at pH 7.0 and 37C
213946
0.000097
2-Ethylphenol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
9013
1.3
2-fluoro-4-hydroxy-3,5-dichlorobiphenyl
Homo sapiens
-
at pH 7.0 and 37C
213949
0.00147
2-Nitrophenol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
1099
12.4
3'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
Homo sapiens
-
at pH 7.0 and 37C
213947
0.000347
3-Ethylphenol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
9036
0.000819
3-Nitrophenol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
1857
4.5
4'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
Homo sapiens
-
at pH 7.0 and 37C
213948
0.000812
4-Ethylphenol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
3312
5.9
4-hydroxy-3,5-dichlorobiphenyl
Homo sapiens
-
at pH 7.0 and 37C
29141
0.001975
4-nitrophenol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
80
0.019
benzyl alcohol
Homo sapiens
O43704, P50225, P50226
isoform SULT1A1, at pH 7.0 and 37C
260
0.000222
m-cresol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
4028
0.000849
o-Cresol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
5195
0.000259
p-Cresol
Arabidopsis thaliana
P52839
at pH 7.5 and 30C
1651
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25
(S)-(-)-1-naphthyl-1-ethanol
pH 5.5, 37C
0.259
2'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
-
at pH 7.0 and 37C
-
0.323
2-fluoro-4-hydroxy-3,5-dichlorobiphenyl
-
at pH 7.0 and 37C
-
0.0775
3'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
-
at pH 7.0 and 37C
-
0.032
3,3',4'-dihydroxy-4-monochlorobiphenyl
-
at pH 7.0 and 37C
-
0.219
4'-fluoro-4-hydroxy-3,5-dichlorobiphenyl
-
at pH 7.0 and 37C
-
0.003
4'-hydroxy-2',3,4-trichlorobiphenyl
-
at pH 7.0 and 37C
0.0006
4'-hydroxy-2,3',4,5'-tetrachlorobiphenyl
-
at pH 7.0 and 37C
0.003
4'-hydroxy-2,3',4-trichlorobiphenyl
-
at pH 7.0 and 37C
0.0054
4'-hydroxy-2,3'-dichlorobiphenyl
-
at pH 7.0 and 37C
0.0007
4'-hydroxy-2,5-dichlorobiphenyl
-
at pH 7.0 and 37C
0.004
4'-hydroxy-3,4-dichlorobiphenyl
-
at pH 7.0 and 37C
0.017
4'-hydroxy-4-monochlorobiphenyl
-
at pH 7.0 and 37C
0.0008
4-hydroxy-2',3,5-trichlorobiphenyl
-
at pH 7.0 and 37C
0.005
4-hydroxy-2,4'-dichlorobiphenyl
-
at pH 7.0 and 37C
0.0034
4-hydroxy-3,3',5-trichlorobiphenyl
-
at pH 7.0 and 37C
0.012
4-hydroxy-3,3'-dichlorobiphenyl
-
at pH 7.0 and 37C
0.0135 - 0.221
4-hydroxy-3,5-dichlorobiphenyl
0.0028
4-tert-octylphenol
-
-
0.014
5,3',4'-dihydroxy-2,3-dichlorobiphenyl
-
at pH 7.0 and 37C
-
0.009
6'-hydroxy-3,3'4-trichlorobiphenyl
-
at pH 7.0 and 37C
-
0.003
adenosine 3',5'-bisphosphate
-
pH 8.0, 37C
0.0007 - 0.159
androsterone
0.0061 - 0.466
dehydroepiandrosterone
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00055
2,6-dibromophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00049
2,6-Dichloro-4-nitrophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00036
2,6-dichlorophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00097
ioxynil
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00016
pentabromophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00015
Pentachlorophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00097
tetrabromobisphenol A
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.0008
tribromophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00037
trichlorophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00021
triiodophenol
Rana catesbeiana
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00002965
substrate 4-methylumbelliferone
0.0000577
-
partially purified enzyme
0.0000596
substrate p-nitrophenol
0.00008
isoform SULT1B1, at pH 7.0 and 37C
0.00018
-
activity with 3-keto-5alpha-petromyzonol, crude larval liver extract
0.000207
substrate p-hydroxybenzoic acid methyl ester
0.000231
-
activity with 5alpha-petromyzonol, crude larval liver extract
0.0002474
substrate p-nitrocatechol
0.0002614
substrate estriol
0.0004035
substrate ethynylestradiol
0.0004193
substrate estradiol
0.0004737
substrate estrone
0.00188
isoform SULT1A2, at pH 7.0 and 37C
0.0019
-
recombinant SULT2B1b, substrate 7beta-hydroxycholesterol
0.002038
-
partially purified enzyme
0.0048
-
recombinant SULT2B1b, substrate cholesterol 7alpha-hydroperoxide
0.0071
-
recombinant SULT2B1b, substrate 7-oxocholesterol
0.0075
-
recombinant SULT2B1b, substrate cholesterol
0.01052
isoform SULT1A1, at pH 7.0 and 37C
0.021
-
recombinant SULT2B1b, substrate 7alpha-hydroxycholesterol
0.11
-
substrate estradiol
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6.5
-
assay at
5.5
-
assay at
5.5 - 6
-
beta-estradiol and dehydroepiandrosterone as substrate
6
-
dehydroepiandrosterone as substrate
7 - 8
-
-
7 - 9
-
recombinant enzyme, expressed in Escherichia coli
7.1 - 7.5
-
assay at
7.4
-
isoform SULT2A1
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
40 - 45
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 50
-
enzyme activity at 25C and 30C drops to 35% and 56%, repective to the maximum activity at 45C, residual activity is determined to be 62% of the maximum at 50C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.12
sequence calculation, SULT2B1b
5.93
sequence calculation, SULT2B1a
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
male white epididymal fat pad, no expression in brown fat
Manually annotated by BRENDA team
-
epidermal keratinocyte
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
endometrial stromal, from 29 patients, reduced or no expression of EST
Manually annotated by BRENDA team
higher expression level of SULT2B1
Manually annotated by BRENDA team
-
medulloblastoma-derived cells
Manually annotated by BRENDA team
-
of male, but not of female mice. Testosterone-dependent, gender-specific expression of estrogen sulfotransferase in adipose tissue
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
-
6 * 28000, SDS-PAGE
30200
-
SDS-PAGE
32000
-
isoenzyme 3alpha-HAST, SDS-PAGE
33000
-
isoenzyme 3beta-HAST, SDS-PAGE
33770
-
calculated from putative amino acid sequence, estimated from cDNA
34000
-
2 * 34000, SDS-PAGE
35000
-
2 * 35000, SDS-PAGE
35930
calculated from putative amino acid sequence, estimated from cDNA
38319
x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation
40000
-
SDS-PAGE
42178
x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation
47000
-
x * 47000, SDS-PAGE
50000
-
gel filtration
53000
-
2 * 53000, SDS-PAGE
67200
-
gel filtration
68000
-
gel filtration
106000
-
gel filtration
135000
-
gel filtration
180000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
-
6 * 28000, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY