Information on EC 2.8.2.2 - alcohol sulfotransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.8.2.2
-
RECOMMENDED NAME
GeneOntology No.
alcohol sulfotransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + an alcohol = adenosine 3',5'-bisphosphate + an alkyl sulfate
show the reaction diagram
primary and secondary alcohols, including aliphatic alcohols, ascorbic acid, chloramphenicol, ephedrine and hydroxysteroids, but not phenolic steroids, can act as acceptors (cf. EC 2.8.2.15 steroid sulfotransferase).
-
-
-
3'-phosphoadenylyl sulfate + an alcohol = adenosine 3',5'-bisphosphate + an alkyl sulfate
show the reaction diagram
random bi-bi mechanism catalytic mechanism of SULTs, many family members have distinct, but overlapping substrate specificities, the enzymes have a sequential catalytic mechanism that is susceptible to substrate inhibition
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
sulfate group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Steroid hormone biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:alcohol sulfotransferase
Primary and secondary alcohols, including aliphatic alcohols, ascorbic acid, chloramphenicol, ephedrine and hydroxysteroids, but not phenolic steroids, can act as acceptors (cf. EC 2.8.2.15 steroid sulfotransferase).
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3-hydroxysteroid sulfotransferase
-
-
-
-
3beta-hydroxy steroid sulfotransferase
-
-
-
-
3beta-hydroxysteroid sulfotransferase
-
-
-
-
5alpha-androstenol sulfotransferase
-
-
-
-
alcohol/hydroxysteroid sulfotransferase
-
-
-
-
cholesterol sulfotransferase
-
-
-
-
dehydroepiandrosterone sulfotransferase
-
-
-
-
DELTA5-3beta-hydroxysteroid sulfokinase
-
-
-
-
estrogen sulfokinase
-
-
-
-
estrogen sulfotransferase
-
-
-
-
HST
-
-
-
-
hydroxysteroid sulfotransferase
-
-
-
-
steroid alcohol sulfotransferase
-
-
-
-
steroid sulfokinase
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-
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steroid sulfotransferase
-
-
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sterol sulfokinase
-
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-
sterol sulfotransferase
-
-
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-
CAS REGISTRY NUMBER
COMMENTARY
9032-76-2
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
guinea pig
-
-
Manually annotated by BRENDA team
human, SULT2A1, cDNA
GenBank
Manually annotated by BRENDA team
human; human, hDHEA-ST, ST2A3, SULT2A1, PDB code 1EFH
-
-
Manually annotated by BRENDA team
isoform SULT1A1; isoform SULT1A3
-
-
Manually annotated by BRENDA team
isozymes SULT2B1a and SULT2B1b
GenBank
Manually annotated by BRENDA team
isozymes SULT2B1a and SULT2B1b
-
-
Manually annotated by BRENDA team
recombinant enzyme expressed in Escherichia coli
-
-
Manually annotated by BRENDA team
SULT1B1
GenBank
Manually annotated by BRENDA team
C57BL/6 mice, seven SULT2A genes including SULT2A1
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-
Manually annotated by BRENDA team
isozymes SULT2B1a and SULT2B1b
SwissProt
Manually annotated by BRENDA team
mouse
SwissProt
Manually annotated by BRENDA team
isoform SULT2B1a; isoform SULT2B1b; SULT2B1; Sprague-Dawley rats, gene SULT2B1, isozymes SULT2B1a and SULT2B1b as a result of an alternative exon I
SwissProt
Manually annotated by BRENDA team
rat; Sprague-Dawley, female
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-
Manually annotated by BRENDA team
rat; Wistar, female
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 1-naphthol
adenosine 3',5'-bisphosphate + 1-naphthyl sulfate
show the reaction diagram
-
SULT1B1
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5'-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
best substrate
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5,5'-tetraiodothyroacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5-triiodothyroacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,5-diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3-iodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 5alpha,6alpha-epoxycholesterol
adenosine 3',5'-bisphosphate + 5alpha,6alpha-epoxycholesteryl sulfate
show the reaction diagram
O00204
-
-
-
?
3'-phosphoadenylyl sulfate + 5beta,6beta-epoxycholesterol
adenosine 3',5'-bisphosphate + 5beta,6beta-epoxycholesteryl sulfate
show the reaction diagram
O00204
-
-
-
?
3'-phosphoadenylyl sulfate + 7-oxo-cholesterol
adenosine 3',5'-bisphosphate + 7-oxo-cholesteryl 3-sulfate
show the reaction diagram
O00204
-
-
-
?
3'-phosphoadenylyl sulfate + 7-oxocholesterol
adenosine 3',5'-bisphosphate + 7-oxocholesterol 3-sulfate
show the reaction diagram
O00204
SULT2B1b, SULT2B1a, and SULT2A1
-
-
?
3'-phosphoadenylyl sulfate + 7alpha-hydroxycholesterol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O00204
-
-
-
?
3'-phosphoadenylyl sulfate + 7alpha-hydroxycholesterol
adenosine 3',5'-bisphosphate + 7alpha-hydroxycholesterol 3-sulfate
show the reaction diagram
O00204
SULT2B1b, the 7alpha derivative is highly preferred
-
-
?
3'-phosphoadenylyl sulfate + 7beta-hydroxycholesterol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O00204
-
-
-
?
3'-phosphoadenylyl sulfate + 7beta-hydroxycholesterol
adenosine 3',5'-bisphosphate + 7beta-hydroxycholesterol 3-sulfate
show the reaction diagram
O00204
SULT2B1b, the 7alpha derivative is highly preferred
-
-
?
3'-phosphoadenylyl sulfate + androsterone
adenosine 3',5'-bisphosphate + androsterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + aristolochic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O00204
herbal preparations containing Aristolochia species lead to progressive nephropathy and urothelial cancer in humans, metabolic activation of aristolichic acid, mutagenic effect, overview, in renal target tissue, substrate of SULT1B1
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
show the reaction diagram
O00204
-
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
show the reaction diagram
Q29YR5
-
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
show the reaction diagram
-
preferred substrate of SULT2B1b
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
show the reaction diagram
O00204
SULT2B1b, SULT2B1a, and SULT2A1
-
-
?
3'-phosphoadenylyl sulfate + cholesterol 7alpha-hydroperoxide
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O00204
SULT2B1b
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
O00204
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
O00204
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
Q29YR5
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
O35400
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
preferred substrate of SULT2B1b
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
O00204
SULT2A1, low activity with SULT2B1b and SULT2B1a
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
Q06520
the sulfation, catalyzed by SULT, is important in the regulation of biological activities of hormones and neurotransmitters, the metabolism of drugs, and the detoxification of xenobiotic toxicants. Sulfation also leads to the bioactivation of procarcinogens. Human SULT2A1 is a major sulfotransferase catalyzing the sulfation of hydroxysteroids and xenobiotic alcohols, mechanisms controlling the regulation of hSULT2A1, overview
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
i.e. PAPS and DHEA
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
Q06520
i.e. PAPS and DHEA
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol sulfate
show the reaction diagram
-
estrogen sulfotransferase expression in adipose tissue is regulated by testosterone, overview
-
-
?
3'-phosphoadenylyl sulfate + genistein
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
show the reaction diagram
Q29YR5
-
-
-
?
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
show the reaction diagram
-
preferred substrate of SULT2B1a
-
-
?
3'-phosphoadenylyl sulfate + raloxifene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + tibolone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + (R)-(-)-2-heptanol
adenosine 3',5'-bisphosphate + (R)-(-)-2-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + (S)-(+)-2-heptanol
adenosine 3',5'-bisphosphate + (S)-(+)-2-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + (S)-(-)-1-phenyl-1-heptanol
adenosine 3',5'-bisphosphate + (S)-(-)-1-phenyl-1-heptyl sulfate
show the reaction diagram
P22789
-
-
-
r
3'-phosphoadenylylsulfate + 1'-hydroxysafrole
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation to a lesser extent
-
-
?
3'-phosphoadenylylsulfate + 1-butanol
adenosine 3',5'-bisphosphate + 1-butanoyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-butanol
adenosine 3',5'-bisphosphate + 1-butanoyl sulfate
show the reaction diagram
-
relative activity 7%
-
-
r
3'-phosphoadenylylsulfate + 1-decanol
adenosine 3',5'-bisphosphate + 1-decyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-dodecanol
adenosine 3',5'-bisphosphate + 1-dodecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-heptanol
adenosine 3',5'-bisphosphate + 1-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-hexanol
adenosine 3',5'-bisphosphate + 1-hexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-hydroxymethylpyrene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation
-
-
?
3'-phosphoadenylylsulfate + 1-methylcyclohexanol
adenosine 3',5'-bisphosphate + 1-methylcyclohexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-nonanol
adenosine 3',5'-bisphosphate + 1-nonyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-octanol
adenosine 3',5'-bisphosphate + 1-octyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-pentanol
adenosine 3',5'-bisphosphate + 1-pentyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-propanol
adenosine 3',5'-bisphosphate + 1-propyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-tetradecanol
adenosine 3',5'-bisphosphate + 1-tetradecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 1-undecanol
adenosine 3',5'-bisphosphate + 1-undecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 11-deoxycorticosterone
adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 11-deoxycorticosterone
adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate
show the reaction diagram
-
relative activity 10%
-
-
r
3'-phosphoadenylylsulfate + 17-hydroxypregnenolone
adenosine 3',5'-bisphosphate + pregn-4-en-3,20-dione 3-sulfate
show the reaction diagram
-
3beta,17alpha-dihydroxypregn-4-en-3,20-dione, isoenzyme 3beta-HST
-
-
r
3'-phosphoadenylylsulfate + 17alpha-ethinylestradiol
adenosine 3',5'-bisphosphate + 17alpha-ethinylestradiol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-hydroxy-catecholestrogen
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
best substrate for this enzyme, also catalyzes the transformation of 4-hydroxy-estrogens and 16-hydroxy-estrogens, but with a lower affinity
-
-
r
3'-phosphoadenylylsulfate + 2-methyl-2-hexanol
adenosine 3',5'-bisphosphate + 1,1-dimethylpentyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 2-propanol
adenosine 3',5'-bisphosphate + 2-propyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3,3'-diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
3'-phosphoadenylylsulfate + 3,5-diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
3'-phosphoadenylylsulfate + 3-ethyl-3-pentanol
adenosine 3',5'-bisphosphate + 3-ethyl-3-pentyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3-keto-5alpha-petromyzonol
adenosine 3',5'-bisphosphate + 7alpha,12alpha,24-trihydroxy-5alpha-cholan-3-one 24 sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + 3-methyl-1-butanol
adenosine 3',5'-bisphosphate + 3-methyl-1-butyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3-O-phenyl-17beta-estradiol
adenosine 3',5'-bisphosphate + 3-phenolic hydroxyl beta-estradiol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 3alpha-hydroxy-5beta-androstan-17-one
adenosine 3',5'-bisphosphate + 5beta-androstan-17-one 3-sulfate
show the reaction diagram
-
relative activity 5%
-
-
?
3'-phosphoadenylylsulfate + 3beta,17alpha-dihydroxy-pregn-5-ene-20-one
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 4%
-
-
r
3'-phosphoadenylylsulfate + 3beta-hydroxy-5beta-androstan-17-one
adenosine 3',5'-bisphosphate + 5beta-androstan-17-one 3-sulfate
show the reaction diagram
-
relative activity 45%
-
-
r
3'-phosphoadenylylsulfate + 4,4'-isopropylidenediphenol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
Q06520
-
-
-
?
3'-phosphoadenylylsulfate + 4-(1,1,3,3-tetramethylbutyl)phenol
adenosine 3',5'-bisphosphate + 4-(1,1,3,3-tetramethylbutyl)phenyl sulfate
show the reaction diagram
Q06520
-
-
-
?
3'-phosphoadenylylsulfate + 4-butylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-butylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-ethylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-ethylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-heptanol
adenosine 3',5'-bisphosphate + 4-heptyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-heptylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-heptylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-hexylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-hexylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-methylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-methylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-methylumbelliferone
adenosine 3',5'-bisphosphate + 4-methylumbelliferone sulfate
show the reaction diagram
O35400
-
-
-
r
3'-phosphoadenylylsulfate + 4-n-nonylphenol
adenosine 3',5'-bisphosphate + 4-n-nonylphenyl sulfate
show the reaction diagram
Q06520
-
-
-
?
3'-phosphoadenylylsulfate + 4-nitrophenol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoform SULT1A1
-
-
?
3'-phosphoadenylylsulfate + 4-octylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-octylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-pentylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-pentylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 4-propylbenzyl alcohol
adenosine 3',5'-bisphosphate + 4-propylbenzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + 5alpha-androstane-3alpha,17beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 20%
-
-
r
3'-phosphoadenylylsulfate + 5alpha-androstane-3beta,17beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 83%
-
-
r
3'-phosphoadenylylsulfate + 5alpha-petromyzonol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
formation of 24-sulfate
-
-
?
3'-phosphoadenylylsulfate + 5alpha-pregnane-3beta,20alpha-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 86%
-
-
r
3'-phosphoadenylylsulfate + 5beta-androstane-3alpha,17beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 7%
-
-
r
3'-phosphoadenylylsulfate + 6-hydroxymethylbenzo[a]pyrene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation
-
-
?
3'-phosphoadenylylsulfate + 7-hydroxymethyl-12-methylbenz[a]anthracene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
P22789
-
-
-
?
3'-phosphoadenylylsulfate + 7-hydroxymethyl-12-methylbenz[a]anthracene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
carcinogen
-
-
?
3'-phosphoadenylylsulfate + 7-hydroxymethyl-12-methylbenz[a]anthracene
adenosine 3',5'-bisphosphate + 7-hydroxymethyl-12-methylbenz[a]anthracene sulfate
show the reaction diagram
P22789
-
-
-
?
3'-phosphoadenylylsulfate + aldosterone
adenosine 3',5'-bisphosphate + aldosterone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + allo-pregnanolone
adenosine 3',5'-bisphosphate + allo-pregnanolone sulfate
show the reaction diagram
-
isoenzyme 3alpha-HST
-
-
r
3'-phosphoadenylylsulfate + alpha-hydroxytamoxifen
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation to a lesser extent
-
-
?
3'-phosphoadenylylsulfate + alpha-hydroxytamoxifen
adenosine 3',5'-bisphosphate + sulfuric acid mono-[(E)-4-[4-(2-dimethylaminoethoxy)-phenyl]-3,4-diphenylbut-3-enyl] ester
show the reaction diagram
-
all enantiomers are substrates: E-(+)-alpha-hydroxytamoxifen, E-(-)-alpha-hydroxytamoxifen, Z-(+)-alpha-hydroxytamoxifen and Z-(-)-alpha-hydroxytamoxifen. The ratio of turnover-number to KM-value is higher with the Z enantiomers than with the E enantiomers
-
-
?
3'-phosphoadenylylsulfate + alpha-naphthol
adenosine 3',5'-bisphosphate + alpha-naphthyl sulfate
show the reaction diagram
-
relative activity 4%
-
-
r
3'-phosphoadenylylsulfate + amyl alcohol
adenosine 3',5'-bisphosphate + amyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androst-5-en-3beta,17alpha-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androst-5-en-3beta,17beta-diol
adenosine 3',5'-bisphosphate + androst-5-en-3beta,17beta-diol sulfate
show the reaction diagram
-
-
-
-
-
3'-phosphoadenylylsulfate + androstenediol
adenosine 3',5'-bisphosphate + androstenediol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androsterone
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + androsterone
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
-
3alpha-hydroxy-5alpha-androstan-17-one
-
-
r
3'-phosphoadenylylsulfate + androsterone
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
-
3alpha-hydroxy-5alpha-androstan-17-one
-
-
r
3'-phosphoadenylylsulfate + androsterone
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
-
isozyme 3alpha-HST
-
-
r
3'-phosphoadenylylsulfate + androsterone
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
-
relative activity 53%
-
-
r
3'-phosphoadenylylsulfate + ascorbic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + benzyl alcohol
adenosine 3',5'-bisphosphate + benzyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + beta-estradiol
adenosine 3',5'-bisphosphate + beta-estradiol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + budesonide-22R
adenosine 3',5'-bisphosphate + budesonide-22R,11 sulfate + budesonide-22R,21 sulfate
show the reaction diagram
O00204
synthetic glucocorticosteroid, used in treatment of asthma and allergic reactions, rhinitis, and inflammatory bowel disease
-
-
r
3'-phosphoadenylylsulfate + budesonide-22S
adenosine 3',5'-bisphosphate + budesonide-22S,11 sulfate + budesonide-22S,21 sulfate
show the reaction diagram
O00204
synthetic glucocorticosteroid, used in treatment of asthma and allergic reactions, rhinitis, and inflammatory bowel disease
-
-
r
3'-phosphoadenylylsulfate + chloramphenicol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cholesterol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
Q29YR5
activity is higher than with pregnenolone, isoform SULT2B1b, poor activity, isoform SULT2B1a
-
-
?
3'-phosphoadenylylsulfate + cis-4-methylcyclohexanol
adenosine 3',5'-bisphosphate + cis-4-methylcyclohexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + corticosterone
adenosine 3',5'-bisphosphate + corticosterone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + corticosterone
adenosine 3',5'-bisphosphate + corticosterone sulfate
show the reaction diagram
-
relative activity 6%
-
-
r
3'-phosphoadenylylsulfate + cortisol
adenosine 3',5'-bisphosphate + cortisol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cortisone
adenosine 3',5'-bisphosphate + cortisone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + cyclohexylmethanol
adenosine 3',5'-bisphosphate + cyclohexylmethyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + D-propranolol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + daidzein
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
Q06520
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
O00204
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
P22789
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
3beta-hydroxyandrost-5-en-17-one
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
isoenzyme 3beta-HST
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
steroid and bile-acid metabolism
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
sulfation is an important pathway in the metabolism of thyroid hormones, sulfated iodothyronines are elevated in nonthyroidal illnesses and in the normal fetal circulation
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
critical step in the provision of substrates for estrogen biosynthesis by the placenta during pregnancy, sulfation represents a major enzymatic reaction in the metabolism, excretion and homeostasis of steroid hormones and bile acids in addition to numerous other endogenous compounds and xenobiotics, regulating steroid hormone bioactivity
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
biotransformation of xenobiotics and hormones through sulfate conjugation, important metabolic pathway
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
biosynthesis of proteoglycans
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
catalyzes sulfonation of exogenous products like drugs and xenobiotics as well as endogenous compounds such as steroids and bile acids by transferring a sulfonate moiety from the cofactor 3'-phosphoadenosine-5'-phosphosulfate to a hydroxyl group of the substrate, this leads to detoxification or elimination because sulfation increases the hydrophilicity of chemical compounds and therefore their excretion, hEST1 also involved in the production of stable precursors for local steroid biosynthesis or in activation of promutagenic estrogen metabolites into carcinogens
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
activation and detoxification of hormones, neurotransmitters, drugs, and carcinogens
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + androst-5-en-17-one 3-sulfate
show the reaction diagram
Escherichia coli, Escherichia coli BL-21
-
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + 1-pentyl sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylylsulfate + diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
3'-phosphoadenylylsulfate + dopamine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoform SULT1A3
-
-
?
3'-phosphoadenylylsulfate + E-(+)-alpha-hydroxytamoxifen
adenosine 3',5'-bisphosphate + sulfuric acid mono-[(E)-4-[4-(2-dimethylaminoethoxy)-phenyl]-3,4-diphenylbut-3-enyl] ester
show the reaction diagram
-
E-(+)-alpha-hydroxytamoxifen is the only substrate, E-(-)-alpha-hydroxytamoxifen, Z-(+)-alpha-hydroxytamoxifen and Z-(-)-alpha-hydroxytamoxifen inhibit sulfation of E-(+)-alpha-hydroxytamoxifen
-
-
?
3'-phosphoadenylylsulfate + ephedrine
adenosine 3',5'-bisphosphate + ephedrine sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + epiandrosterone
adenosine 3',5'-bisphosphate + 5alpha-androstan-17-one 3-sulfate
show the reaction diagram
-
3beta-hydroxy-5alpha-androstan-17-one
-
-
r
3'-phosphoadenylylsulfate + estriol
adenosine 3',5'-bisphosphate + estriol sulfate
show the reaction diagram
O35400
-
-
-
r
3'-phosphoadenylylsulfate + estriol
adenosine 3',5'-bisphosphate + estriol sulfate
show the reaction diagram
-
poor substrate, relative activity 8% with beta-estradiol as 100%
-
-
r
3'-phosphoadenylylsulfate + estrone
adenosine 3',5'-bisphosphate + estrone sulfate
show the reaction diagram
O35400
-
-
-
r
3'-phosphoadenylylsulfate + estrone
adenosine 3',5'-bisphosphate + estrone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + ethanol
adenosine 3',5'-bisphosphate + ethyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + ethanol
adenosine 3',5'-bisphosphate + ethyl sulfate
show the reaction diagram
-
high activity with SULT1A2, SULT1B1, SULT2A1 and SULT1A3. SULT1A3 might be the predominant form for the sulphonation of ethanol
-
-
?
3'-phosphoadenylylsulfate + ethynylestradiol
adenosine 3',5'-bisphosphate + ethynylestradiol sulfate
show the reaction diagram
O35400
-
-
-
r
3'-phosphoadenylylsulfate + genistein
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
Q06520
-
-
-
?
3'-phosphoadenylylsulfate + hycanthone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation
-
-
?
3'-phosphoadenylylsulfate + hydrocortisone
adenosine 3',5'-bisphosphate + hydrocortisone sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + isoamyl alcohol
adenosine 3',5'-bisphosphate + isoamyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + L-propranolol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + L-tyrosinemethyl ester
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
relative activity 9%
-
-
r
3'-phosphoadenylylsulfate + methanol
adenosine 3',5'-bisphosphate + methyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + minoxidil
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
antihypertensive agent and hair growth stimulator, activation by sulfation
-
-
?
3'-phosphoadenylylsulfate + N-hydroxy-2-acetylaminofluorene
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
isoenzyme DHEA-ST, sulfation and bioactivation to a lesser extent
-
-
?
3'-phosphoadenylylsulfate + ouabain
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + p-hydroxybenzoic acid methyl ester
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O35400
-
-
-
r
3'-phosphoadenylylsulfate + p-nitrocatechol
adenosine 3',5'-bisphosphate + p-nitrocatechol sulfate
show the reaction diagram
O35400
-
-
-
r
3'-phosphoadenylylsulfate + p-nitrophenol
adenosine 3',5'-bisphosphate + p-nitrophenyl sulfate
show the reaction diagram
O35400
-
-
-
r
3'-phosphoadenylylsulfate + pregn-5-ene-3beta,20beta-diol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate
show the reaction diagram
-
isoenzyme 3beta-HST
-
-
r
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + 5-pregnen-20-one 3-sulfate
show the reaction diagram
-
relative activity 61%, 3beta-hydroxy-5-pregnen-20-one
-
-
r
3'-phosphoadenylylsulfate + pregnenolone
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
Q29YR5
isoform SULT2B1a, isoform SULT2B1b
-
-
?
3'-phosphoadenylylsulfate + R-(+)-1-naphthyl-1-ethanol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
P22789
-
-
-
?
3'-phosphoadenylylsulfate + R-(+)-1-naphthyl-1-ethanol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
S-(-)-enantiomer is not a substrate for the enzyme
-
-
?
3'-phosphoadenylylsulfate + R-(+)-2-naphthyl-1-ethanol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
both enantiomers are substrates for the enzyme
-
-
?
3'-phosphoadenylylsulfate + retinol
adenosine 3',5'-bisphosphate + retinol sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + S-(-)-2-naphthyl-1-ethanol
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
both enantiomers are substrates for the enzyme
-
-
?
3'-phosphoadenylylsulfate + testosterone
adenosine 3',5'-bisphosphate + testosterone 3-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + testosterone
adenosine 3',5'-bisphosphate + testosterone 3-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + testosterone
adenosine 3',5'-bisphosphate + testosterone 3-sulfate
show the reaction diagram
-
relative activity 2%
-
-
r
3'-phosphoadenylylsulfate + testosterone
adenosine 3',5'-bisphosphate + testosterone 3-sulfate
show the reaction diagram
-
17beta-hydroxyandrost-4-en-3-one
-
-
r
3'-phosphoadenylylsulfate + testosterone
adenosine 3',5'-bisphosphate + testosterone 3-sulfate
show the reaction diagram
-
17-hydroxyl of testosterone
-
-
r
3'-phosphoadenylylsulfate + tetraiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
3'-phosphoadenylylsulfate + trans-4-methylcyclohexanol
adenosine 3',5'-bisphosphate + trans-4-methylcyclohexyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + tridecan-1-ol
adenosine 3',5'-bisphosphate + tridecyl sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
sulfation inactivates the substrate by addition of a sulfuryl moiety to the 4'-hydroxyl group
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
O35400
exhibits sulfotransferase activity with phenolic hydroxy groups of steroids and artificial substrates, best acceptor substrate is estrogen
-
-
-
additional information
?
-
-
major form of sulfotransferase, catalyzes the formation og genotoxic and carcinogenic sulfuric acid esters from hydroxymethyl polycyclic aromatic hydrocarbons
-
-
-
additional information
?
-
-
estrone and phenolic steroids are no substrates
-
-
-
additional information
?
-
-
activation and detoxification of both endogenous and exogenous chemicals, isozyme AST-RB2 high activity to both hydroxysteroids and amines, no activity to lithocholate
-
-
-
additional information
?
-
-
cortisol, corticosterone, betamathasone and dexamethasone are no substrates
-
-
-
additional information
?
-
-
cholesterol, phenol, 4-aminophenol, 2-aminophenol, 3-aminophenol, hydroquinone, 1-naphthol, 2-naphthol, 4-acetamidophenol. pentachlorophenol, m-cresol, p-cresol, 4-ethylphenol, 4-propylphenol, 4-sec-butylphenol, 4-octylphenol, 4-dodecylphenol, 9-phenanthrol, and estrone are no substrates
-
-
-
additional information
?
-
-
preferentially catalyzes the sulfurylation of the 3beta.-OH group of DELTA5-steroids
-
-
-
additional information
?
-
-
3beta-hydroxysteroids with a DELTA5 double bond serve as substrates while androgens, estrogens, corticosteroids, p-nitrophenol and 3,4-dihydroxyphenylalanine do not serve as substrates
-
-
-
additional information
?
-
-
hepatic clearance and metabolism of sex steroids
-
-
-
additional information
?
-
P22789
(S)-(-)-1-naphthyl-1-ethanol is not a substrate for STa
-
-
-
additional information
?
-
-
2 isoenzymes of 3-hydroxysteroid sulfotransferase
-
-
-
additional information
?
-
-
specificity of the enzyme is not limited to hydroxysteroids, a wide range of alcohols serve as sulfate acceptors
-
-
-
additional information
?
-
-
does not utilize cholesterol, 2-naphthylamine, 2-naphthol, taurolithocholic acid, estrone, progesterone and N-hydroxy-2-acetylaminofluorene as sulfate acceptors
-
-
-
additional information
?
-
-
inactive towards estrogens, cholesterol and ergocalciferol, maximum activity is observed when the steroid is of the allo-series and possesses a 3beta-hydroxyl and 17-oxo function, estrone, estradiol, cortisol, 11beta-hydroxy-pregn-4-ene-3-20-dione, beta-naphthol, alpha-naphthylamine, bilirubin and diethylstilbestrol are no substrates for the purified enzyme
-
-
-
additional information
?
-
-
isoform DHEA-ST, sulfate conjugation involved in bioactivation of compounds to reactive electrophilic forms, the hydroxymethyl group is sulfated and undergoes subsequent sponaneous rearrangement to release the sulfate moiety and generate an electrophilic benzylic carbocation that can react with cellular nucleophiles such as DNA, RNA, and protein
-
-
-
additional information
?
-
-
is a target for transcriptional induction by the vitamin D receptor
-
-
-
additional information
?
-
-
regulation of human hepatic hydroxysteroid sulfotransferase gene expression by the peroxisome proliferator-activated receptor alpha transcription factor
-
-
-
additional information
?
-
Q29YR5
no activity with dehydroepiandrosterone, isoform SULT2b1a, no activity with dehydroepiandrosterone, isoform SULT2b1b
-
-
-
additional information
?
-
-
SULT2B1 isoforms show selectivity for the sulfation of 3beta-hydroxysteroids including dehydroepiandrosterone and pregnenolone, and demonstrate little activity with 3alpha-hydroxysteroids and estrogens, SULT2B1a possesses a higher pregnenolone sulfation activity than SULT2B1b whereas SULT2B1b has a higher cholesterol sulfation activity, SULT2B1b is also capable of conjugating several drugs and xenobiotics including genistein, tibolone, and raloxifene
-
-
-
additional information
?
-
-
SULTs play a role in the metabolism of regulatory hormones, drugs, and carcinogens, they catalyze the sulfonation of simple phenols, estradiol, and thyroid hormones, as well as environmental xenobiotics and drugs, overview, role for 3'-phosphoadenylyl sulfate in priming the conformation of substrate binding loops, overview, substrate specificity and binding structure of SULT1B1, overview
-
-
-
additional information
?
-
-
SULT2B1b catalyzes the sulfonation of 3beta-hydroxysteroid hormones and cholesterol, whereas SULT2B1a preferentially catalyzes pregnenolone sulfonation
-
-
-
additional information
?
-
O00204
SULT2B1b is selective for the sulfation of 3beta-hydroxysteroids, such as DHEA, pregnenolone, androstenediol, and 5alpha-androstane-3beta,17beta-diol, no sulfation is detectable with 3alpha-hydroxysteroids or estrogens as substrates
-
-
-
additional information
?
-
O00204
SULT2B1b sulfates oxosterols, overview
-
-
-
additional information
?
-
-
EST is an enzyme that seems to play an important role in the growth of hormone-dependent tumors of the breast, prostate, and endometrium, EST expression is downregulated in endometrial stromal sarcomas, ESS. If the enzyme EST is missing in ESS, it is possible that the intratumoral levels of bio-active estrogen are high, and ESS tumor growth and progression are to be expected, overview
-
-
-
additional information
?
-
-
hydroxysteroid sulfotransferase SULT2A enzymes play important roles in hepatic steroid and xenobiotic metabolism
-
-
-
additional information
?
-
-
sulfotransferase-catalyzed sulfation plays an important role in various neuronal functions such as homeostasis of catecholamine neurotransmitters and hormones
-
-
-
additional information
?
-
-
pentachlorophenols, tetrachlorophenols, and trichlorophenols are substrates of isozyme SULT2A1, while mono- and dichlorophenol are poor substrates, cf. EC 2.8.2.15
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + 3,3',5'-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
best substrate
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5,5'-tetraiodothyroacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5-triiodothyroacetic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,3',5-triiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3,5-diiodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 3-iodothyronine
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + 7-oxocholesterol
adenosine 3',5'-bisphosphate + 7-oxocholesterol 3-sulfate
show the reaction diagram
O00204
SULT2B1b, SULT2B1a, and SULT2A1
-
-
?
3'-phosphoadenylyl sulfate + androsterone
adenosine 3',5'-bisphosphate + androsterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + aristolochic acid
adenosine 3',5'-bisphosphate + ?
show the reaction diagram
O00204
herbal preparations containing Aristolochia species lead to progressive nephropathy and urothelial cancer in humans, metabolic activation of aristolichic acid, mutagenic effect, overview
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate
show the reaction diagram
Q29YR5
-
-
-
?
3'-phosphoadenylyl sulfate + cholesterol
adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
show the reaction diagram
O00204
SULT2B1b, SULT2B1a, and SULT2A1
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
O00204
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
Q29YR5
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
O35400
-
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
O00204
SULT2A1, low activity with SULT2B1b and SULT2B1a
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
Q06520
the sulfation, catalyzed by SULT, is important in the regulation of biological activities of hormones and neurotransmitters, the metabolism of drugs, and the detoxification of xenobiotic toxicants. Sulfation also leads to the bioactivation of procarcinogens. Human SULT2A1 is a major sulfotransferase catalyzing the sulfation of hydroxysteroids and xenobiotic alcohols, mechanisms controlling the regulation of hSULT2A1, overview
-
-
?
3'-phosphoadenylyl sulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
show the reaction diagram
-
-
-
-
?
3'-phosphoadenylyl sulfate + estradiol
adenosine 3',5'-bisphosphate + estradiol sulfate
show the reaction diagram
-
estrogen sulfotransferase expression in adipose tissue is regulated by testosterone, overview
-
-
?
3'-phosphoadenylyl sulfate + pregnenolone
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate
show the reaction diagram
Q29YR5
-
-
-
?
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
O00204
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
P22789
-
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
steroid and bile-acid metabolism
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
sulfation is an important pathway in the metabolism of thyroid hormones, sulfated iodothyronines are elevated in nonthyroidal illnesses and in the normal fetal circulation
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
critical step in the provision of substrates for estrogen biosynthesis by the placenta during pregnancy, sulfation represents a major enzymatic reaction in the metabolism, excretion and homeostasis of steroid hormones and bile acids in addition to numerous other endogenous compounds and xenobiotics, regulating steroid hormone bioactivity
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
biotransformation of xenobiotics and hormones through sulfate conjugation, important metabolic pathway
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
biosynthesis of proteoglycans
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
catalyzes sulfonation of exogenous products like drugs and xenobiotics as well as endogenous compounds such as steroids and bile acids by transferring a sulfonate moiety from the cofactor 3'-phosphoadenosine-5'-phosphosulfate to a hydroxyl group of the substrate, this leads to detoxification or elimination because sulfation increases the hydrophilicity of chemical compounds and therefore their excretion, hEST1 also involved in the production of stable precursors for local steroid biosynthesis or in activation of promutagenic estrogen metabolites into carcinogens
-
-
r
3'-phosphoadenylylsulfate + dehydroepiandrosterone
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
show the reaction diagram
-
activation and detoxification of hormones, neurotransmitters, drugs, and carcinogens
-
-
r
additional information
?
-
-
is a target for transcriptional induction by the vitamin D receptor
-
-
-
additional information
?
-
-
regulation of human hepatic hydroxysteroid sulfotransferase gene expression by the peroxisome proliferator-activated receptor alpha transcription factor
-
-
-
additional information
?
-
-
SULT2B1 isoforms show selectivity for the sulfation of 3beta-hydroxysteroids including dehydroepiandrosterone and pregnenolone, and demonstrate little activity with 3alpha-hydroxysteroids and estrogens, SULT2B1a possesses a higher pregnenolone sulfation activity than SULT2B1b whereas SULT2B1b has a higher cholesterol sulfation activity, SULT2B1b is also capable of conjugating several drugs and xenobiotics including genistein, tibolone, and raloxifene
-
-
-
additional information
?
-
-
SULTs play a role in the metabolism of regulatory hormones, drugs, and carcinogens, they catalyze the sulfonation of simple phenols, estradiol, and thyroid hormones, as well as environmental xenobiotics and drugs, overview
-
-
-
additional information
?
-
-
EST is an enzyme that seems to play an important role in the growth of hormone-dependent tumors of the breast, prostate, and endometrium, EST expression is downregulated in endometrial stromal sarcomas, ESS. If the enzyme EST is missing in ESS, it is possible that the intratumoral levels of bio-active estrogen are high, and ESS tumor growth and progression are to be expected, overview
-
-
-
additional information
?
-
-
hydroxysteroid sulfotransferase SULT2A enzymes play important roles in hepatic steroid and xenobiotic metabolism
-
-
-
additional information
?
-
-
sulfotransferase-catalyzed sulfation plays an important role in various neuronal functions such as homeostasis of catecholamine neurotransmitters and hormones
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
-
activity is increased by
Mg2+
Q29YR5
-
additional information
-
crude enzyme activity is markedly stimulated by divalent cations like Fe2+, Co2+ and Mn2+, this effect is not observed with purified enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2,6-dibromophenol
-
-
2,6-Dichloro-4-nitrophenol
-
-
2,6-Dichloro-4-nitrophenol
-
-
2,6-dichlorophenol
-
-
3'-phosphoadenylyl sulfate
-
substrate inhibition of SULT2A1, structure analysis of SULT2A1/DHEA and SULT2A1/PAP or SULT2A1/ADT, overview
3,5-dibromo-4-hydroxy-benzoic acid (6-chloro-4-oxo-4H-chromen-3-ylmethylene)-hydrazide
-
DBHM
3,5-dibromo-4-hydroxybenzoic acid (6,8-dichloro-4-oxo-4H-chromen-3-ylmethylene) hydrazide
-
DBHD
4-n-octylphenol
-
-
4-tert-octylphenol
-
-
adenosine 3',5'-bisphosphate
-
enzyme appears to be highly suspectible to product inhibition
adenosine 3',5'-bisphosphate
-
competitive inhibition
adenosine 5'-(beta,gamma-imido) triphosphate
-
i.e. AMP-PNP, a non-hydrolysable ATP analogue
androsterone
-
-
bis(2-ethylhexyl)phthalate
-
-
dehydroepiandrosterone
P22789
substrate inhibition
dehydroepiandrosterone
-
substrate inhibition of SULT2A1, structure analysis of SULT2A1/DHEA and SULT2A1/PAP or SULT2A1/ADT, overview
diisodecyl phthalate
-
-
E-(-)-alpha-hydroxytamoxifen
-
inhibits sulfation of E-(+)-alpha-hydroxytamoxifen
-
p-chloromercuribenzoate
-
-
pentabromophenol
-
-
Pentachlorophenol
-
most potent competitor
pyridoxal 5'-phosphate
-
a competitive inhibitor for sulfotransferases
S-(-)-1-naphthyl-1-ethanol
P22789
-
S-(-)-1-naphthyl-1-ethanol
-
inhibitor of the sulfation of dehydroepiandrosterone
tetrabromobisphenol A
-
52% inhibition at 0.001 mM
tetrachlorobisphenol A
-
45% inhibition at 0.001 mM
tribromophenol
-
-
trichlorophenol
-
-
triiodophenol
-
-
Z-(+)-alpha-hydroxytamoxifen
-
inhibits sulfation of E-(+)-alpha-hydroxytamoxifen
-
Z-(-)-alpha-hydroxytamoxifen
-
inhibits sulfation of E-(+)-alpha-hydroxytamoxifen
-
ioxynil
-
-
additional information
-
EDTA has no effect
-
additional information
-
cholesterol is no inhibitor
-
additional information
-
isoprenaline is poor inhibitor of SULT1B1
-
additional information
-
environmental contaminants may exert effects on neuronal function both by direct inhibition of sulfotransferase enzymes and by interrupting the supply of PAPS
-
additional information
-
rifampicin-liganded PXR suppresses SULT2A1 expression by interfering with HNF4alpha activity, overview
-
additional information
-
nonylphenol and bisphenol A (non-halogenated phenol and phenolic compounds) have little effect on T3 sulfation (inhibition at 0.001 mM = 4% and 9%, respectively)
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cysteine
-
essential for optimal activity
NaCl
-
leads to a greater activation of the cDNA-expressed DHEA ST when assayed with dehydroepiandrosterone
glutathione
-
activity of the purified enzyme is slightly enhanced
additional information
-
stimulated by nonionic detergents
-
additional information
-
methotrexate and 6-(4-chlorophenyl)imidazo [2,1-b][1,3] thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime induction of hSULT2A1 through interacting with androstane receptor and retinoid X receptor alpha mediated by the IR2 element, mechanism, overview
-
additional information
-
liver X receptor antagonist TO-901317 induces estrogen sulfotransferase expression in female liver, but not in the adipose tissue
-
additional information
-
rifampicin induces SULT2A1 expression about 12fold in a manner dependent on 5'-flanking regions containing rifampicin-responsive information as a hepatocyte nuclear factor 4, HNF4, binding site. Upregulation of SULT2A1 does not occur in cells, that do not contain the binding site. Binding of HNF4 to the HNF4-54 region of the endogenous SULT2A1 gene leads to increased SULT2A1 mRNA production and enzyme activity, overview
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.12
(12-methyltetraphen-7-yl)methanol
-
pH 5.5, 37C
0.96
(R)-(+)-1-naphthyl-1-ethanol
P22789
pH 7.0, 37C
0.15
(R)-(+)-1-phenyl-1-heptanol
P22789
pH 7.0, 37C
4.7
(R)-(-)-2-heptanol
-
pH 5.5, 37C
4.9
(S)-(+)-2-heptanol
-
pH 5.5, 37C
0.31
(S)-(-)-1-phenyl-1-heptanol
P22789
pH 7.0, 37C
1.7
1-amylalcohol
-
pH 5.5, 37C
3
1-butanol
-
-
3
1-butanol
-
pH 5.5, 37C
106
1-butanol
-
pH 5.5, 37C
0.31
1-Decanol
-
pH 5.5, 37C
0.33
1-Dodecanol
-
pH 5.5, 37C
2.2
1-Heptanol
-
pH 5.5, 37C
4.3
1-Hexanol
-
pH 5.5, 37C
26.8
1-methylcyclohexanol
-
pH 5.5, 37C
0.37
1-nonanol
-
pH 5.5, 37C
0.77
1-Octanol
-
pH 5.5, 37C
1.7
1-Pentanol
-
pH 7.5, 37C
19.2
1-Pentanol
-
pH 5.5, 37C
24
1-propanol
-
pH 7.5, 37C
610
1-propanol
-
pH 5.5, 37C
0.23
1-Tetradecanol
-
pH 5.5, 37C
0.23
1-tridecanol
-
pH 5.5, 37C
0.26
1-undecanol
-
pH 5.5, 37C
0.52
11-deoxycorticosterone
-
pH 7.5, 37C
0.000897
17-hydroxypregnenolone
-
pH 7.7, 37C
24.6
2-methyl-2-hexanol
-
pH 5.5, 37C
17
2-propanol
-
pH 7.5, 37C
0.00109
3'-phosphoadenylyl sulfate
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00013
3'-phosphoadenylylsulfate
-
pH 7.5, 37C
0.0025
3'-phosphoadenylylsulfate
-
-
0.012
3'-phosphoadenylylsulfate
-
pH 5.5, 37C
0.013
3'-phosphoadenylylsulfate
-
pH 8.0, 37C
0.062
3'-phosphoadenylylsulfate
-
-
0.1
3'-phosphoadenylylsulfate
-
pH 5.5, 37C
0.00477
3,3',5-Triiodothyroacetic acid
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00022
3,3',5-triiodothyronine
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00295
3,3'-diiodothyronine
-
pH 6.0, 37C, isoform SULT2A1
0.00973
3,3'-diiodothyronine
-
pH 6.0, 37C, isoform SULT1E1
0.00484
3,5-diiodothyronine
-
pH 6.0, 37C, isoform SULT2A1
40.3
3-ethyl-3-pentanol
-
pH 5.5, 37C
1.2
3-methyl-1-butanol
-
pH 7.5, 37C
0.00063
3beta,20beta-pregnenediol
-
pH 7.5, 37C
0.0043
4,4'-isopropylidenediphenol
Q06520
pH 7.4, 37C
0.0051
4-(1,1,3,3-tetramethylbutyl)phenol
Q06520
pH 7.4, 37C
0.07
4-butylbenzyl alcohol
-
pH 5.5, 37C
0.16
4-butylbenzyl alcohol
-
pH 5.5, 37C
0.67
4-Ethylbenzyl alcohol
-
pH 5.5, 37C
7.2
4-heptanol
-
pH 5.5, 37C
0.19
4-heptylbenzyl alcohol
-
pH 5.5, 37C
0.2
4-hexylbenzyl alcohol
-
pH 5.5, 37C
0.07
4-methylbenzyl alcohol
-
pH 5.5, 37C
2.19
4-methylbenzyl alcohol
-
pH 5.5, 37C
0.0715
4-n-nonylphenol
Q06520
pH 7.4, 37C
0.29
4-octylbenzyl alcohol
-
pH 5.5, 37C
0.12
4-pentylbenzyl alcohol
-
pH 5.5, 37C
0.4
4-propylbenzyl alcohol
-
pH 5.5, 37C
0.008
5alpha-petromyzonol
-
-
0.72
7-hydroxymethyl-12-methylbenz[a]anthracene
P22789
pH 7.0, 37C
0.17
aldosterone
-
pH 7.5, 37C
0.000083
allopregnanolone
-
pH 7.7, 37C
0.000175
androsterone
-
pH 7.7, 37C
0.0002
androsterone
-
pH 7.0, 25C, recombinant mutant M137I
0.0007
androsterone
-
pH 7.0, 25C, recombinant mutant M137V; pH 7.0, 25C, recombinant mutant Y238W
0.0014
androsterone
-
pH 7.0, 25C, recombinant mutant M137I/Y238A; pH 7.0, 25C, recombinant mutant Y238A; pH 7.0, 25C, recombinant wild-type enzyme
0.0015
androsterone
-
pH 7.0, 25C, recombinant mutant Y238F
0.002
androsterone
-
pH 7.0, 25C, recombinant mutant M137W/Y238A
0.0036
androsterone
-
pH 7.0, 25C, recombinant mutant M137V/Y238A
0.0096
androsterone
-
pH 7.0, 25C, recombinant mutant M137W
25.8
benzyl alcohol
-
pH 5.5, 37C
0.035
beta-estradiol
-
-
0.035
beta-estradiol
-
pH 5.5, 37C
0.29
beta-estradiol
-
pH 5.5, 37C
0.0209
budesonide-22R
-
pH 7.4, 37C
0.0111
budesonide-22S
-
pH 7.4, 37C
0.0017
cholesterol
Q29YR5
pH 7.5, 37C, recombinant isozyme SULT2B1b
0.0025
cholesterol
-
pH 7.5, 37C
4.5
cis-4-methylcyclohexanol
-
pH 5.5, 37C
0.29
cortisol
-
pH 7.5, 37C
0.44
cortisol
-
pH 7.5, 37C
2
cyclohexylmethanol
-
pH 5.5, 37C
0.1673
daidzein
Q06520
pH 7.4, 37C
0.0006
dehydroepiandrosterone
-
pH 7.5, 37C, recombinant truncated mutant SULT2B1
0.0007
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant Y238W
0.0011
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant Y238F
0.001157
dehydroepiandrosterone
-
pH 7.7, 37C
0.0013
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant M137I; pH 7.0, 25C, recombinant mutant M137W
0.0019
dehydroepiandrosterone
-
pH 7.5, 37C
0.0019
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant M137V
0.002
dehydroepiandrosterone
Q06520
pH 7.4, 37C
0.0025
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant wild-type enzyme
0.0033
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant Y238A
0.0036
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant M137I/Y238A
0.0038
dehydroepiandrosterone
-
pH 7.5, 37C, recombinant wild-type, full-length SULT2B1
0.0042
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant M137V/Y238A
0.006
dehydroepiandrosterone
-
pH 8.0, 37C
0.0065
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant M137V/Y238A
0.0086
dehydroepiandrosterone
-
pH 7.5, 37C
0.012
dehydroepiandrosterone
-
-
0.012
dehydroepiandrosterone
-
pH 5.5, 37C
0.0179
dehydroepiandrosterone
Q29YR5
pH 7.5, 37C, recombinant isozyme SULT2B1a
0.0465
E-(+)-alpha-hydroxytamoxifen
-
pH 8.0, 37C
0.0358
E-(-)-alpha-hydroxytamoxifen
-
pH 8.0, 37C
-
0.35
estriol
-
pH 7.5, 37C
42
ethanol
-
-
42
ethanol
-
pH 5.5, 37C
47
ethanol
-
pH 7.5, 37C
0.041
genistein
Q06520
pH 7.4, 37C
1
Hexanol
-
pH 7.5, 37C
1.2
Isoamylalcohol
-
pH 5.5, 37C
0.000257
pregnenolone
-
pH 7.7, 37C
0.0016
pregnenolone
-
pH 7.5, 37C
0.0089
pregnenolone
Q29YR5
pH 7.5, 37C, recombinant isozyme SULT2B1a
0.24
R-(+)-1-naphthyl-1-ethanol
-
pH 7.0, 37C
0.25
R-(+)-2-naphthyl-1-ethanol
-
pH 7.0, 37C
-
0.54
S-(-)-2-naphthyl-1-ethanol
-
pH 7.0, 37C
0.07
testosterone
-
-
0.07
testosterone
-
pH 5.5, 37C
0.00136
tetraiodothyronine
-
pH 6.0, 37C, isoform SULT1E1
5.1
trans-4-methylcyclohexanol
-
pH 5.5, 37C
0.00463
triiodothyronine
-
pH 6.0, 37C, recombinant isoform SULT1E1
0.00607
triiodothyronine
-
pH 6.0, 37C, isoform SULT1E1
0.00704
triiodothyronine
-
pH 6.0, 37C, recombinant isoform SULT2A1
0.0143
triiodothyronine
-
pH 6.0, 37C, isoform SULT2A1
0.0669
Z-(+)-alpha-hydroxytamoxifen
-
pH 8.0, 37C
-
0.0405
Z-(-)-alpha-hydroxytamoxifen
-
pH 8.0, 37C
-
3.9
minoxidil
-
pH 7.5, 37C
additional information
additional information
Q29YR5
steady-state kinetic analysis of recombinant isozymes SULT2B1a and SULT2B1b
-
additional information
additional information
-
the proline- and serine-rich carboxyl extension of SULT2B1b is an important site in the kinetic activity of this SULT isoform, overview
-
additional information
additional information
-
substrate binding kinetics
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.283
1-butanol
-
pH 7.5, 37C
0.417
1-Hexanol
-
pH 7.5, 37C
0.45
1-Pentanol
-
pH 7.5, 37C
0.183
1-propanol
-
pH 7.5, 37C
0.183
11-deoxycorticosterone
-
pH 7.5, 37C
0.217
2-propanol
-
pH 7.5, 37C
0.417
3-methyl-1-butanol
-
pH 7.5, 37C
0.133
aldosterone
-
pH 7.5, 37C
0.583
beta-estradiol
-
pH 7.5, 37C
0.183
cortisol
-
pH 7.5, 37C
0.15
cortisone
-
pH 7.5, 37C cosubstrate ethanol
2
dehydroepidandrosterone
-
pH 7.5, 37C
-
0.167
estriol
-
pH 7.5, 37C
0.0833
methanol
-
pH 7.5, 37C
0.467
testosterone
-
pH 7.5, 37C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.25
(S)-(-)-1-naphthyl-1-ethanol
P22789
pH 5.5, 37C
0.0028
4-tert-octylphenol
-
-
0.003
adenosine 3',5'-bisphosphate
-
pH 8.0, 37C
0.0007
androsterone
-
pH 7.0, 25C, recombinant wild-type enzyme
0.0012
androsterone
-
pH 7.0, 25C, recombinant mutant Y238W
0.0022
androsterone
-
pH 7.0, 25C, recombinant mutant Y238F
0.0038
androsterone
-
pH 7.0, 25C, recombinant mutant M137W
0.0078
androsterone
-
pH 7.0, 25C, recombinant mutant M137V
0.02
androsterone
-
pH 7.0, 25C, recombinant mutant M137I
0.159
androsterone
-
pH 7.0, 25C, recombinant mutant M137I/Y238A
0.0061
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant wild-type enzyme
0.0071
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant M137W
0.012
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant Y238F
0.015
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant Y238W
0.036
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant M137I
0.045
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant Y238A
0.053
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant M137V
0.17
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant M137I/Y238A
0.466
dehydroepiandrosterone
-
pH 7.0, 25C, recombinant mutant M137V/Y238A
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00055
2,6-dibromophenol
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00049
2,6-Dichloro-4-nitrophenol
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00036
2,6-dichlorophenol
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00097
ioxynil
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00016
pentabromophenol
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00015
Pentachlorophenol
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00097
tetrabromobisphenol A
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.0008
tribromophenol
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00037
trichlorophenol
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
0.00021
triiodophenol
-
in 0.1 M sodium phosphate, pH 7.2, and 2 mM EDTA for 30 min at 16C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.00002965
O35400
substrate 4-methylumbelliferone
0.0000577
-
partially purified enzyme
0.0000596
O35400
substrate p-nitrophenol
0.00018
-
activity with 3-keto-5alpha-petromyzonol, crude larval liver extract
0.000207
O35400
substrate p-hydroxybenzoic acid methyl ester
0.000231
-
activity with 5alpha-petromyzonol, crude larval liver extract
0.0002474
O35400
substrate p-nitrocatechol
0.0002614
O35400
substrate estriol
0.0004035
O35400
substrate ethynylestradiol
0.0004193
O35400
substrate estradiol
0.0004737
O35400
substrate estrone
0.0019
-
recombinant SULT2B1b, substrate 7beta-hydroxycholesterol
0.002038
-
partially purified enzyme
0.0048
-
recombinant SULT2B1b, substrate cholesterol 7alpha-hydroperoxide
0.0071
-
recombinant SULT2B1b, substrate 7-oxocholesterol
0.0075
-
recombinant SULT2B1b, substrate cholesterol
0.021
-
recombinant SULT2B1b, substrate 7alpha-hydroxycholesterol
0.11
-
substrate estradiol
0.15
-
substrate n-butanol
0.15
-
-
additional information
-
continuous fluorimetric assay for alcohol sulfotransferase whose sensitivity is comparable to that of the end-point radioactive assay. Assay procedure can be adapted for high-throughput assay using a microplate reader
additional information
-
continuous fluorimetric assay for alcohol sulfotransferase whoses sensitivity is comparable to that of the end-point radioactive assay. Assay procedure can be adapted for high-throughput assay using a microplate reader
additional information
-
continuous fluorimetric assay for alcohol sulfotransferase whose sensitivity is comparable to that of the end-point radioactive assay. Assay procedure can be adapted for high-throughput assay using a microplate reader
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5 - 6
-
beta-estradiol and dehydroepiandrosterone as substrate
5.5 - 6.5
-
assay at
5.5
-
assay at
6
-
dehydroepiandrosterone as substrate
7 - 8
-
-
7 - 9
-
recombinant enzyme, expressed in Escherichia coli
7
-
assay at
7.1 - 7.5
-
assay at
7.4
-
isoform SULT2A1
7.5
-
1-butanol as substrate
7.5
Q29YR5
assay at
7.5
-
assay at
7.5
-
assay at
8
-
broad pH optimum centered on pH 8.0
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 9
-
enzyme activity at pH 5.0 is 6% of the maximum in the optimum range, 50% of activity maximum at pH 6.0, no activity is detected at either pH 4.0 or 10.0
6 - 10
O35400
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
-
assay at
37
Q29YR5
assay at
37
-
assay at
40 - 45
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25 - 50
-
enzyme activity at 25C and 30C dropps to 35% and 56%, repective to the maximum activity at 45C, residual activity is determined to be 62% of the maximum at 50C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5
-
preparative isoelectric focusing in sucrose gradients with ampholites in the range pH 3.0-8.0
5
-
isoelectric focusing, sucrose gradient in presence of 2.5% ampholines pH 5.0-7.0
5.12
Q29YR5
sequence calculation, SULT2B1b
5.93
Q29YR5
sequence calculation, SULT2B1a
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
fetal, low activity, isoform SULT1A1
Manually annotated by BRENDA team
-
fetal, high activity in choroid plexus, isoform SULT1A1
Manually annotated by BRENDA team
Q29YR5
isoform SULT2B1a
Manually annotated by BRENDA team
O35400
higher expression level of SULT2B1
Manually annotated by BRENDA team
Q29YR5
SULT2B1a is only expressed in brain and testis
Manually annotated by BRENDA team
-
enzyme localization in neural fibers, posterior region, and dorsal part of the brain, overview
Manually annotated by BRENDA team
-
cancer tissue
Manually annotated by BRENDA team
-
adult, isoform SULT1A1, adult, isoform SULT1A3
Manually annotated by BRENDA team
-
epidermal keratinocyte
Manually annotated by BRENDA team
-
male white epididymal fat pad, no expression in brown fat
Manually annotated by BRENDA team
-
primary culture
Manually annotated by BRENDA team
-
hepatocellular carcinoma cell line
Manually annotated by BRENDA team
-
fetal intestinal villi
Manually annotated by BRENDA team
-
fetal, high activity, isoform SULT1A3
Manually annotated by BRENDA team
Q29YR5
isoform SULT2B1b
Manually annotated by BRENDA team
O35400
lower expression level of SULT2B1
Manually annotated by BRENDA team
Q29YR5
main expression of SULT2B1b
Manually annotated by BRENDA team
-
present in proximal and distal tubules, loops of Henle and collecting ducts, but at no time expressed in the vascular glomerulus
Manually annotated by BRENDA team
-
fetal, low activity, isoform SULT1A1
Manually annotated by BRENDA team
Q29YR5
isoform SULT2B1b, main expression of SULT2B1b
Manually annotated by BRENDA team
-
adult and fetal, isoform SULT1A1
Manually annotated by BRENDA team
-
expression of three of the SULT2A isoforms is abundant in pre-pubertal male and female mouse liver but is essentially silenced in the livers of adult male mice, overview
Manually annotated by BRENDA team
-
prostate epithelial cells, prostate benign prostatic hyperplasia, prostate adenocarcinoma and LNCaP prostate adenocarcinoma cells
Manually annotated by BRENDA team
-
endometrial stromal, from 29 patients, reduced or no expression of EST
Manually annotated by BRENDA team
Q29YR5
isoform SULT2B1b, main expression of SULT2B1b
Manually annotated by BRENDA team
-
SULT2B1b, but not SULT2B1a
Manually annotated by BRENDA team
O35400
higher expression level of SULT2B1
Manually annotated by BRENDA team
-
medulloblastoma-derived cells
Manually annotated by BRENDA team
-
interstitial cells of the testis
Manually annotated by BRENDA team
O35400
testis-specific isoform
Manually annotated by BRENDA team
Q29YR5
isoform SULT2B1a, SULT2B1a is only expressed in brain and testis
Manually annotated by BRENDA team
-
of male, but not of female mice. Testosterone-dependent, gender-specific expression of estrogen sulfotransferase in adipose tissue
Manually annotated by BRENDA team
additional information
-
no expression of SULT2B1b in alveolar tissue and in liver cytosol
Manually annotated by BRENDA team
additional information
Q29YR5
no expression of SULT2B1b in placental, prostate and breast tissues in rodents
Manually annotated by BRENDA team
additional information
O35400
no expression of SULT2B1b in placental, prostate and breast tissues in rodents
Manually annotated by BRENDA team
additional information
Q29YR5
tissue distribution of isozymes SULT2B1a and SULT2B1b expression, overview
Manually annotated by BRENDA team
additional information
-
no estrogen sulfotransferase expression in brown adipose tissue, differential expression of estrogen sulfotransferase in fat and liver, overview
Manually annotated by BRENDA team
additional information
-
no expression in 293T cells
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
isozyme SULT2B1b, T47D or MCF-7 breast cancer cells, in prostate epithelial cells, prostate benign prostatic hyperplasia, prostate adenocarcinoma and LNCaP prostate adenocarcinoma cells isozyme SULT2B1b is expressed only in the cytosol
Manually annotated by BRENDA team
-
the proline- and serine-rich carboxyl extension of SULT2B1b is an important site in the nuclear translocation of this SULT isoform, overview
Manually annotated by BRENDA team
-
isozyme SULT2B1b, T47D or MCF-7 breast cancer cells, SULT2B1b is not tightly bound in the nuclei
Manually annotated by BRENDA team
-
the proline- and serine-rich carboxyl extension of SULT2B1b is an important site in the nuclear translocation of this SULT isoform, overview
Manually annotated by BRENDA team
additional information
-
a unique 3'-extension of SULT2B1b is required for nuclear localization in human BeWo placental choriocarcinoma cells, the nuclear localization is stimulated by forskolin treatment in BeWo cells and serine phosphorylation in the 3'-extension
-
Manually annotated by BRENDA team
additional information
-
subcellular localization of isozymes SULT2B1a and SULT2B1b, overview
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30200
-
SDS-PAGE
645784
32000
-
isoenzyme 3alpha-HAST, SDS-PAGE
645781
33000
-
isoenzyme 3beta-HAST, SDS-PAGE
645781
33600
P22789
SDS-PAGE
645793
33770
-
calculated from putative amino acid sequence, estimated from cDNA
645780, 645783
35930
Q06520
calculated from putative amino acid sequence, estimated from cDNA
645798
40000
-
SDS-PAGE
645792
50000
-
gel filtration
645792
67200
-
gel filtration
645801
68000
-
gel filtration
645783
106000
-
gel filtration
645777
135000
-
gel filtration
645781
180000
-
gel filtration; sucrose density gradient centrifugation
645774
180000
-
gel filtration
645776
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 47000, SDS-PAGE
?
Q29YR5
x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation
dimer
-
2 * 53000, SDS-PAGE
dimer
-
2 * 35000, SDS-PAGE
dimer
-
2 * 34000, SDS-PAGE
hexamer
-
6 * 28000, SDS-PAGE
additional information
-
detailed structural analysis and comparison of SULT family enzymes, comparison of substrate binding structures of SULT1B1, overview, chemical library preparation and ligand binding screens
additional information
-
SULT2B1 isoforms possess a 50 amino acid N-terminal extension that is enriched in serine and proline residues
additional information
-
SULT2B1b has an extension at the proline- and serine-rich carboxyl end of about 53 amino acids, structure and function, the proline- and serine-rich carboxyl extension of SULT2B1b is an important site in the immunogenicity, nuclear translocation, kinetic activity, and thermostability of this SULT isoform, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
-
recombinant wild-type, full-length SULT2B1b, but not truncated SULT2B1b, is phosphorylated by casein kinase or Cdc2 protein kinase in vitro
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DHEA-ST
-
human cholesterol sulfotransferase SULT2B1b
-
purified recombinant mutants M137I and M137W, hanging drop vapor diffusion method at 18C, 0.002 ml hanging drops by mixing equal volumes of protein solution and a reservoir solution containing 1.6 M (NH4)2SO4, 100 mM NaCl, and 0.1 M HEPES at pH 7.5, 3-7 days, X-ray diffraction structure determination and analysis at 2.6 and 3.0 A resolution, respectively, modeling
-
purified SULT1B1 is crystallized in the presence of 2 mM PAP using the hanging drop method at 20C by mixing for SULT1B1: 0.002 ml of the protein solution with 0.002 ml of the reservoir solution containing 0.1 M Bis-Tris, pH 6.5, 0.2 M ammonium sulfate and 16%-20% PEG 4000
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5
-
unstable at low pH
645773
6 - 9
-
stable for 30 min at 37C
645773
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 43
-
50% inactivation temperature for COS-expressed enzyme 42C
645786
4 - 43
-
50% inactivation temperature for SULT1E1 41.0C, for SULT2A1 45.5C
645789
42
-
30 min, the activity of the recombinant wild-type, full-length SULT2B1b is reduced by 30%, but the activity of the recombinant truncated SULT2B1bmutant is reduced 3.5fold more
673295
45
-
90% basal activity remains after 15 min
645791
additional information
-
the proline- and serine-rich carboxyl extension of SULT2B1b is an important site in the thermostability of this SULT isoform, overview
673295
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
partially purified enzyme loses 30% of upon freeze-thawing, activity can be stabilized by adenosine 3',5'-bisphosphate
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, activity towards dehydroepiandrosterone as acceptor substrate is stable for several months
-
-20C, partially purified enzyme in 50% glycerol or ethylene glycol loses 30% of activity overnight
-
-80C, stable for several months
-
4C, activity declines by 60% after 4 weeks
-
4C, loses 10% activity per week upon storage
-
4C, partially purified enzyme loses 30% of activity overnight
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme partially from brain by native PAGE
-
partially, 5 ST activities in liver preparation
-
recombinant GST-tagged SULT2B1 by glutathione affinity chromatography
-
recombinant His-tagged full-length SULT2B1b and of truncated SULT2B1b lacking proline- and serine-rich carboxyl extension from Escherichia coli strain DH5alpha by nickel affinity chromatography
-
recombinant SULT2A1 from Escherichia coli by anion exchange chromatography, ultrafiltration, and hydroxyapatite chromatography
-
; recombinant GST-tagged isozymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
Q29YR5
native and recombinant protein
P22789
sulfotransferase 1
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression analysis in brain enzyme extracts
-
; cloned and expressed in Escherichia coli
-
; SULT2A1 bacterially expressed as His tagged protein in Escherichia coli
Q06520
cDNA encoding alcohol/hydroxysteroid sulfotransferase h-ST-a cloned from a liver cDNA library
-
cDNA encoding liver DHEA-ST isolated from Uni-Zap XR human liver cDNA library cloned, expressed in green monkey COS-7 cells
-
DHEA ST and SULT2A1 cloned and expressed in COS-1 cells
-
DHEA ST cloned from cDNA and expressed in COS-1 cells
-
DHEA-ST cloned and expressed in glutathione sulfotransferase fusion form in Escherichia coli
-
DHEA-ST cloned and expressed in Salmonella typhimurium, Escherichia coli and COS cells
-
expression of GFP-tagged wild-type allozymes and mutant truncated SULT2B1a and SULT2B1b variants lacking the SULT2B1 proline-rich tail in COS-1 cells; isozymes SULT2B1a and SULT2B1b are encoded by a single SULT2B1 gene as a result of alternative transcription initiation and alternative splicing, genotyping of African-American and Caucasian-American individuals, transient expression of GFP-tagged SULT2B1a and SULT2B1b in COS-1 cells, in vitro transcription and translation of truncated SULT2B1a and SULT2B1b variants that lack the SULT2B1 proline-rich tail using rabbit reticulocyte lysate
-
expression of GST-tagged SULT2B1; expression of SULT2B1b eliminates the cytotoxic effect of 7-oxo-cholesterol on 293T cells, which are normally devoid of SULT2B1b
-
expression of His-tagged full-length SULT2B1b and of truncated SULT2B1b lacking proline- and serine-rich carboxyl extension in Escherichia coli strain DH5alpha, expression and serine phosphorylation of SULT2B1b in transfected BeWo cells, overview
-
expression of SULTs in Escherichia coli strain BL21 DE3, in Salmonella typhimurium strain TA1538, and in chinese hamster V79 cells, and application of aristolochic acid, mutagenicity with expression of different SULT isozymes and allozymes of SULT1A1, detailed overview
O00204
SULT2A1 real time RT-PCR expression analysis in presence of different plasticizers
-
SULT2A1, expression in Caco-2 cells and analysis of the transcriptional regulation mechanism of hSULT2A1 promoter, quantitative enzyme expression analysis. Constitutive androstane receptor and retinoid X receptor alpha are involved in the transcriptional regulation of hSULT2A1, overview
-
SULT2A1, expression in Escherichia coli
-
SULT2B1a and SULT2B1b, DNA and amino acid sequence determination and analysis, genetic structure, the two different isozymes are encoded by the gene SULT2B1 utilizing different start sites of transcription resulting in the incorporation of different first exons, SULT2B1a and SULT2B1b are 350 and 365 amino acids in length, respectively, and the last 342 amino acids are identical, expression of His-tagged or GST-tagged SULT2B1b in Escherichia coli, expression of SULT2B1a and SULT2B1b in COS-1 cells
-
transient expression of SULT2A1 in Hep-G2 cells, expression analysis in presence or absence of rifampicin and/or PXR, regulation, overview
-
cDNA of placental EST cloned, COS-7 cells transfected with mpEST
O35400
cloned and expressed in Escherichia coli
-
expression in HepG2 cells or CaCo-2 cells
-
presence of seven SULT2A genes, including SULT2A1, within a cluster on chromosome 7, DNA and amino acid sequence determination and analysis, age- and sex-dependent expressions of the seven genes, overview
-
SULT2B1a and SULT2B1b, DNA and amino acid sequence determination and analysis, genetic structure
O35400
cloning of AST-RB2 (ST2A8) from cDNA library, expressed in Escherichia coli
-
; gene SULT2B1, isozymes SULT2B1a and SULT2B1b as a result of an alternative exon I, DNA and amino acid sequence determination and analysis, genetic structure, expression analysis, comparison of sequence and genetic structure, overview, overexpression of GST-tagged isozymes in Escherichia coli strain BL21(DE3)
Q29YR5
STa cloned into pET-3c vector and expressed in Escherichia coli BL21
P22789
SULT2B1a and SULT2B1b, DNA and amino acid sequence determination and analysis, genetic structure
Q29YR5
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
iodothyronine sulfating activity in the liver cytosol is increased in tadpoles at premetamorphic (stages IX-X) and metamorphic climax (stage XX) stages, and in adult frogs
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D176N
-
naturally occuring polymorphism in SULT2B1a
L36S
-
naturally occuring polymorphism in SULT2B1a
M137I
-
site-directed mutagenesis, substrate binding structure, the Ki for androsterone is increased 28.6fold compared to the wild-type enzyme, overview
M137K
-
site-directed mutagenesis, inactive mutant
M137K/Y238A
-
site-directed mutagenesis, inactive mutant
M137V
-
site-directed mutagenesis, substrate binding structure, the Ki for androsterone is increased 5fold and for dehydroepiandrosterone 9fold compared to the wild-type enzyme, overview
M137V/Y238A
-
site-directed mutagenesis, the mutant shows no substrate inhibition by dehydroepiandrosterone
M137W
-
site-directed mutagenesis, substrate binding structure, the Ki for androsterone is increased 11.1fold compared to the wild-type enzyme, overview
P330L
-
naturally occuring polymorphism in SULT2B1a
R215H
-
naturally occuring polymorphism in SULT2B1a
V260A
-
site-directed mutagenesis, a monomeric mutant
V260A/Y238A
-
site-directed mutagenesis, a monomeric mutant, shows increased Ki for dehydroepiandosterone compared to the wild-type enzyme, no inhibition by androsterone
Y238A
-
site-directed mutagenesis, exhibits no substrate inhibition with ADT as substrate, the Ki value of DHEA increases 7.4fold compared with that of wild-type SULT2A1, no inhibition by androsterone
Y238F
-
site-directed mutagenesis, the Ki values of DHEA and ADT for Y238F SULT2A1 mutant increase for approximately 2 to 3fold compared with those of the wild type enzyme
M137W/Y238A
-
site-directed mutagenesis, the mutant shows no substrate inhibition by dehydroepiandrosterone
additional information
-
construction of a truncated mutant SULT2B1b lacking the 53 amino acid-proline- and serine-rich carboxyl extension, the mutant enzyme shows decreased Km for dehydroepiandrosterone and a kcat reduced by 91% compared to the wild-type full-length SULT2B1b
additional information
-
construction of truncated SULT2B1a and SULT2B1b variants that lack the SULT2B1 proline-rich tail, genotype-to-phenotype approach to identify and characterize common sequence variation in SULT2B1, determination and analysis of 100 polymorphisms in African-American and Caucasian-American individuals, including four nonsynonymous coding single nucleotide polymorphisms and one 6-base pair deletion, within the shared region of the open reading frame, overview, construction of truncated SULT2b1a and SULT2B1b variants by site-directed mutagenesis to introduce a translation termination codon immediately before the final 53 amino acids at the C terminus of the protein. Pharmacogenomics, gene sequence variation and functional genomics, determination of 100 polymorphisms, including four nonsynonymous coding single nucleotide polymorphisms and one 6-base pair deletion, in African-American and Caucasian-American individuals, overview
additional information
-
SULT2A1 downreguletion by siRNA, and progressive promoter deletion, expression analysis. An IR2 element of the hSULT2A1 promoter region mediates the methotrexate induction of hSULT2A1 through interacting with androstane receptor and retinoid X receptor alpha
Y238W
-
site-directed mutagenesis, the Ki values of DHEA and ADT for Y238W SULT2A1 mutant increase for approximately 2 to 3fold compared with those of the wild type enzyme
additional information
-
male estrogen sulfotransferase-knockout mice develop increased epididymal fat accumulation with enlarged adipocyte size, phenotype, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
diagnostics
-
EST expression is a prognostic factor in breast cancer
medicine
-
hEST1 involved in the production of stable precursors for local steroid biosynthesis or in activation of promutagenic estrogen metabolites into carcinogens
medicine
-
isoenzyme EST plays important roles in regulating the in situ production of estrogens in breast carcinoma tissue, immunoreactivity of the enzymes is a potent prognostic factor for cancer
medicine
-
diagnostic of liver diseases, in chronic hepatitis the immunopositive area of DHEA-ST is significantly increased compared to normal liver
medicine
P22789
model studies on the carcinogenesis of benzylic and allylic alcohols