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Literature summary for 2.7.4.27 extracted from

  • Smith, C.M.; Duff, S.M.; Chollet, R.
    Partial purification and characterization of maize-leaf pyruvate, orthophosphate dikinase regulatory protein: a low-abundance, mesophyll-chloroplast stromal protein (1994), Arch. Biochem. Biophys., 308, 200-206.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast mesophyll-chloroplast stroma Zea mays 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
2 * 48000, SDS-PAGE, gel filtration, pH 7.0-7.5 Zea mays
48000
-
4 * 48000, SDS-PAGE, gel filtration, pH 8.3 Zea mays
90000
-
gel filtration, pH 7.0-7.5 Zea mays
180000
-
gel filtration, pH 8.3 Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified, ammonium sulfate precipitation, dye-ligand chromatography (Blue Sepharose), gel filtration, anion exchange chromatography Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[pyruvate,phosphate dikinase]phosphate + phosphate Thr456 phosphorylated (inactive) Zea mays pyruvate,phosphate dikinase + diphosphate active ?

Subunits

Subunits Comment Organism
homodimer 2 * 48000, SDS-PAGE, gel filtration, pH 7.0-7.5 Zea mays
homotetramer 4 * 48000, SDS-PAGE, gel filtration, pH 8.3 Zea mays

Synonyms

Synonyms Comment Organism
pyruvate,orthophosphate dikinase regulatory protein
-
Zea mays