Information on EC 2.7.4.27 - [pyruvate, phosphate dikinase]-phosphate phosphotransferase

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The expected taxonomic range for this enzyme is: Magnoliophyta

EC NUMBER
COMMENTARY
2.7.4.27
-
RECOMMENDED NAME
GeneOntology No.
[pyruvate, phosphate dikinase]-phosphate phosphotransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
[pyruvate, phosphate dikinase] phosphate + phosphate = [pyruvate, phosphate dikinase] + diphosphate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
[pyruvate, phosphate dikinase] phosphate:phosphate phosphotransferase
The enzyme from the plants maize and Arabidopsis is bifunctional and also catalyses the phosphorylation of pyruvate, phosphate dikinase (EC 2.7.9.1), cf. EC 2.7.11.32, [pyruvate, phosphate dikinase] kinase [2-5].
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
AtRP1
O49562
-
bifunctional regulatory protein of pyruvate,orthophosphate dikinase
-
-
PDRP1
-
-
gene name
-
PPDK regulatory protein
-
-
PPDK regulatory protein
-
bifunctional serine/threonine kinase-phosphatase
pyruvate, orthophosphate dikinase regulatory protein
-
-
pyruvate, orthophosphate dikinase regulatory protein 1
O49562
bifunctional protein kinase/protein phosphatase
pyruvate,orthophosphate dikinase regulatory protein
-
-
pyruvate,Pi dikinase regulatory protein
-
-
pyruvate,Pi dikinase regulatory protein
-
bifunctional, catalyses phosphorylation and dephosphorylation of pyruvate,Pi dikinase
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cv. Dekalb XL81
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
[pyruvate, phosphate dikinase]phosphate + phosphate
pyruvate, phosphate dikinase + diphosphate
show the reaction diagram
O49562
His458 and Thr456 phosphorylated (inactive), strict requirement for the threonyl phosphate, unable to dephosphorylate Ser-P at this same position
His458 phosphorylated (active)
-
?
[pyruvate, phosphate dikinase]phosphate + phosphate
pyruvate, phosphate dikinase + diphosphate
show the reaction diagram
-
Thr residue phosphorylated (inactive)
active
-
?
[pyruvate, phosphate dikinase]phosphate + phosphate
pyruvate, phosphate dikinase + diphosphate
show the reaction diagram
-
Thr456 and His458 phosphorylated (inactive)
His458 phosphorylated (active)
-
?
[pyruvate,phosphate dikinase]phosphate + phosphate
pyruvate,phosphate dikinase + diphosphate
show the reaction diagram
-
His458 and Thr456 phosphorylated (inactive)
His458 phosphorylated (active)
-
?
[pyruvate,phosphate dikinase]phosphate + phosphate
pyruvate,phosphate dikinase + diphosphate
show the reaction diagram
-
Thr residue phosphorylated (inactive)
active
-
ir
[pyruvate,phosphate dikinase]phosphate + phosphate
pyruvate,phosphate dikinase + diphosphate
show the reaction diagram
-
Thr456 phosphorylated (inactive)
active
-
?
[pyruvate,Pi dikinase]phosphate + phosphate
pyruvate,Pi dikinase + diphosphate
show the reaction diagram
-
a His residue and a Thr residue phosphorylated (inactive)
one His residue phosphorylated (active)
-
?
[pyruvate,Pi dikinase]phosphate + phosphate
pyruvate,Pi dikinase + diphosphate
show the reaction diagram
-
phosphorylated at a Thr residue, inactive, additionally catalytically phosphorylated substrate (at His residue) is a much poorer substrate
active
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-nitro-5-thiocyanatobenzoate
-
-
ADP
-
-
ADP
-
competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue)
ADP-beta-S
-
competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue)
AMP
-
-
AMP
-
competitive to phosphate
CDP
-
-
Cibacron blue
-
competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue)
diphosphate
-
competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue)
GDP
-
-
Phenylglyoxal
-
-
pyridoxal 5'-phosphate
-
-
pyruvate,Pi dikinase phosphorylated at a His residue
-
competitive to substrate [pyruvate,Pi dikinase]phosphate (phosphorylated at a Thr residue)
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.65
phosphate
-
pH 8.3, temperature not specified in the publication
0.67
phosphate
-
pH 8.3., 30°C
0.0007
[pyruvate,Pi dikinase]phosphate
-
phosphorylated at a Thr residue, pH 8.3, temperature not specified in the publication
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.085
ADP
-
pH 8.3, temperature not specified in the publication
0.08
ADP-beta-S
-
Ki-value below 0.08 mM, pH 8.3, temperature not specified in the publication
0.4
AMP
-
pH 8.3, temperature not specified in the publication
0.005
Cibacron blue
-
pH 8.3, temperature not specified in the publication
0.16
diphosphate
-
pH 8.3, temperature not specified in the publication
1.7
pyridoxal 5'-phosphate
-
pH 8.3, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.1
-
broad optimum pH 7.8-8.4, 68% activity at pH 7.2
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
O49562
green tissues
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
mesophyll-chloroplast stroma
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
90000
-
gel filtration, pH 7.0-7.5
645448
90000
-
pH 7.5, gel filtration
721404
180000
-
gel filtration, pH 8.3
645448
180000
-
pH 8.3, gel filtration
721404
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 45000, SDS-PAGE
?
-
x * 40000, SDS-PAGE
homodimer
-
2 * 48000, SDS-PAGE, gel filtration, pH 7.0-7.5
homodimer
-
90000 at pH 7.5
homotetramer
-
180000 at pH 8.3
homotetramer
-
4 * 48000, SDS-PAGE, gel filtration, pH 8.3
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stabilized at 25°C by phosphate, ATP, Blue Dextran and Cibacron blue
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
immobilized metal ion affinity chromatography (Ni2+)
O49562
ammonium sulfate precipitation, dye ligand chromatography (agarose-Blue Dextran), gel filtration
-
immobilized metal ion affinity chromatography (Ni2+)
-
partially purified
-
partially purified, ammonium sulfate precipitation, dye ligand chromatography (Blue-Sepharose, agarose-blue dextran), gel filtration
-
partially purified, ammonium sulfate precipitation, dye-ligand chromatography (Blue Sepharose), gel filtration, anion exchange chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-tagged and GFP-fusion proteins expressed in Escherichia coli BL21(DE3)
O49562
His-tagged protein expressed in Escherichia coli NM522
-