Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoic acid | - |
Rattus norvegicus | |
L-isoleucine | branched chain alpha-amino acids bind at the active site, competitive inhibition mechanism against substrates phosphocreatine and ADP, inhibition kinetics | Rattus norvegicus | |
L-leucine | branched chain alpha-amino acids bind at the active site, competitive inhibition mechanism against substrates phosphocreatine and ADP, inhibition kinetics | Rattus norvegicus | |
L-valine | branched chain alpha-amino acids bind at the active site, competitive inhibition mechanism against substrates phosphocreatine and ADP, inhibition kinetics | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Rattus norvegicus | |
0.3 | - |
ADP | pH 7.5, 37°C | Rattus norvegicus | |
0.8 | - |
phosphocreatine | pH 7.5, 37°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | Rattus norvegicus | key enzyme of energy homeostasis | ADP + phosphocreatine | - |
r | |
additional information | Rattus norvegicus | enzyme inhibition, e.g. by branched chain alpha-amino acids, might contribute to the brain damage maple syrup urine disease MSUD | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
21-day-old Wistar rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain cortex | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + creatine | - |
Rattus norvegicus | ADP + phosphocreatine | - |
r | |
ATP + creatine | key enzyme of energy homeostasis | Rattus norvegicus | ADP + phosphocreatine | - |
r | |
additional information | enzyme inhibition, e.g. by branched chain alpha-amino acids, might contribute to the brain damage maple syrup urine disease MSUD | Rattus norvegicus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Rattus norvegicus | |
ATP | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
L-isoleucine | versus phosphocreatine, pH 7.5, 37°C | Rattus norvegicus | |
14 | - |
L-valine | versus phosphocreatine, pH 7.5, 37°C | Rattus norvegicus | |
16 | - |
L-leucine | versus phosphocreatine, pH 7.5, 37°C | Rattus norvegicus | |
21 | - |
L-isoleucine | versus ADP, pH 7.5, 37°C | Rattus norvegicus | |
22 | - |
L-leucine | versus ADP, pH 7.5, 37°C | Rattus norvegicus | |
23 | - |
L-valine | versus ADP, pH 7.5, 37°C | Rattus norvegicus |