Cloned (Comment) | Organism |
---|---|
gene pgk-A, quantitative RT-PCR enzyme expression analysis, recombinant expression of N-terminally His-tagged wild-type and truncated mutant enzymes and of the 80 amino acids long peptide corresponding to the PGKA N-terminal insertion in Escherichia coli strain BL21(DE3) | Trypanosoma cruzi |
gene pgk-B, quantitative RT-PCR enzyme expression analysis | Trypanosoma cruzi |
gene pgk-C, quantitative RT-PCR enzyme expression analysis | Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a mutant not possessing the 80 amino-acids insertion in its N-terminal domain, the PGKA N-terminal 80 amino-acids insertion is deleted by site-directed mutagenesis, obtaining an internally truncated rPGKA with a molecular weight of 48 kDa | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | isozyme PGKC is partly inhibited by 0.13 mM | Trypanosoma cruzi | |
suramin | - |
Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Trypanosoma cruzi | |
0.01 | - |
ATP | isozyme PGKC, pH 7.5, 22°C | Trypanosoma cruzi | |
0.174 | - |
3-phospho-D-glycerate | native isozyme PGKA, pH 7.5, 22°C | Trypanosoma cruzi | |
0.192 | - |
3-phospho-D-glycerate | isozyme PGKC, pH 7.5, 22°C | Trypanosoma cruzi | |
0.217 | - |
ATP | native isozyme PGKA, pH 7.5, 22°C | Trypanosoma cruzi | |
0.236 | - |
ATP | recombinant wild-type isozyme PGKA, pH 7.5, 22°C | Trypanosoma cruzi | |
0.63 | - |
3-phospho-D-glycerate | recombinant truncated isozyme PGKA mutant, pH 7.5, 22°C | Trypanosoma cruzi | |
0.64 | - |
ATP | recombinant truncated isozyme PGKA mutant, pH 7.5, 22°C | Trypanosoma cruzi | |
0.85 | - |
3-phospho-D-glycerate | recombinant wild-type isozyme PGKA, pH 7.5, 22°C | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trypanosoma cruzi | 5829 | - |
glycosome | - |
Trypanosoma cruzi | 20015 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
- |
Trypanosoma cruzi |
48000 | - |
recombinant truncated mutant enzyme, gel filtration | Trypanosoma cruzi |
52000 | - |
- |
Trypanosoma cruzi |
57000 | - |
recombinant wild-type enzyme, gel filtration | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | Trypanosoma cruzi | - |
ADP + 3-phospho-D-glyceroyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
isozyme PGKC, gene pgk-C | - |
Trypanosoma cruzi | Q95PK8 | isozyme PGKB, gene pgk-B | - |
Trypanosoma cruzi | Q95PK9 | isozyme PGKA, gene pgk-A | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged wild-type and truncated mutant enzymes and 80 amino acids long peptide corresponding to the PGKA N-terminal insertion to homogeneity from Escherichia coli strain BL21(DE3) by metal affinity chromatography | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | - |
Trypanosoma cruzi | - |
epimastigote | - |
Trypanosoma cruzi | - |
additional information | differential expression of the PGK isoenzymes in amastigote, trypomastigote and epimastigote forms, quantitative RT-PCR expression analysis, overview | Trypanosoma cruzi | - |
trypomastigote | - |
Trypanosoma cruzi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.12 | - |
purified truncated enzyme mutant, pH 7.5, 22°C | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Trypanosoma cruzi | ADP + 3-phospho-D-glyceroyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, wild-type isozyme PGKC, SDS-PAGE | Trypanosoma cruzi |
monomer | 1 * 52000, wild-type isozyme PGKA, SDS-PAGE, 1 * 48000, truncated enzyme mutant, SDS-PAGE | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
PGK | - |
Trypanosoma cruzi |
pgk-B | - |
Trypanosoma cruzi |
PGKA | - |
Trypanosoma cruzi |
PGKB | - |
Trypanosoma cruzi |
PGKC | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Trypanosoma cruzi |
8 | - |
recombinant isozyme PGKA | Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0101 | - |
suramin | versus ATP, pH 7.5, 22°C | Trypanosoma cruzi | |
0.0121 | - |
suramin | versus 3-phospho-D-glycerate, pH 7.5, 22°C | Trypanosoma cruzi | |
0.27 | - |
ATP | isozyme PGKC, pH 7.5, 22°C | Trypanosoma cruzi |