Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the enzyme shows hyperbolic saturation kinetics for 3-phospho-D-glycerate, ATP and 3-phospho-D-glyceroyl phosphate. For ADP it shows a high affinity binding for the reaction in the reverse direction and a low affinity inhibitory effect for the forward as well as the reverse reaction | Saccharolobus solfataricus | |
0.085 | - |
ADP | 70°C, pH 6.5 | Saccharolobus solfataricus | |
0.54 | - |
3-phospho-D-glycerate | 70°C, pH 6.5 | Saccharolobus solfataricus | |
5.6 | - |
3-phospho-D-glyceroyl phosphate | 70°C, pH 6.5 | Saccharolobus solfataricus | |
9.7 | - |
ATP | 70°C, pH 6.5 | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | P50317 | - |
- |
Saccharolobus solfataricus P2 | P50317 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate | an ordered mechanism is assumed where 3-phospho-D-glycerate and 3-phospho-D-glyceroyl phosphate bind before ADP and ATP, respectively | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Saccharolobus solfataricus | ADP + 3-phospho-D-glyceroyl phosphate | - |
r | |
ATP + 3-phospho-D-glycerate | - |
Saccharolobus solfataricus P2 | ADP + 3-phospho-D-glyceroyl phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PGK | - |
Saccharolobus solfataricus |
SSO0527 | locus name | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.14 | - |
ADP | 70°C, pH 6.5 | Saccharolobus solfataricus |
General Information | Comment | Organism |
---|---|---|
physiological function | gluconeogenic pathway | Saccharolobus solfataricus |