Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Phaeodactylum tricornutum |
Protein Variants | Comment | Organism |
---|---|---|
C214S | similar Vmax as wild-type, mutant behaves in a similar way as wild-type towards oxidants | Phaeodactylum tricornutum |
C21S | similar Vmax as wild-type, mutant displays slightly lower affinity toward 3-phospho-D-glycerate, mutant behaves in a similar way as wild-type towards oxidants | Phaeodactylum tricornutum |
C312S | similar Vmax as wild-type, mutant behaves in a similar way as wild-type towards oxidants | Phaeodactylum tricornutum |
C58S | similar Vmax as wild-type, mutant displays slightly lower affinity toward 3-phospho-D-glycerate, mutant is notably more sensitive to diamide oxidation than wild-type | Phaeodactylum tricornutum |
C77S | similar Vmax as wild-type, mutant displays slightly lower affinity toward 3-phospho-D-glycerate, mutant is notably more sensitive to diamide oxidation than wild-type | Phaeodactylum tricornutum |
C95S | similar Vmax as wild-type, mutant displays slightly lower affinity toward 3-phospho-D-glycerate, mutant is notably more sensitive to diamide oxidation than wild-type | Phaeodactylum tricornutum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Diamide | - |
Phaeodactylum tricornutum | |
glutathione | - |
Phaeodactylum tricornutum | |
hydrogen peroxide | - |
Phaeodactylum tricornutum | |
sodium nitroprusside | - |
Phaeodactylum tricornutum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Phaeodactylum tricornutum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaeodactylum tricornutum | - |
- |
- |
Oxidation Stability | Organism |
---|---|
loses its activity when stored in the absence of of a reducing agent | Phaeodactylum tricornutum |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Phaeodactylum tricornutum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47.5 | - |
pH 8, 30°C, purified enzyme | Phaeodactylum tricornutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Phaeodactylum tricornutum | ADP + 3-phospho-D-glyceroyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | gel filtration, 1 * 45000 | Phaeodactylum tricornutum |
Synonyms | Comment | Organism |
---|---|---|
PGKase-1 | - |
Phaeodactylum tricornutum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Phaeodactylum tricornutum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Phaeodactylum tricornutum |