Cloned (Comment) | Organism |
---|---|
gene MAPKKK4.1, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree, quantitative RT-PCR expression analysis, recombinant expression in transgenic Nicotiana benthamiana leaves under the driving of CaMV35S promoter, recombinant coexpression with substrate proteins MKK3 and MKK6 in Saccharomyces cerevisiae strain AH109 cells | Brassica napus |
gene MAPKKK4.2, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree, quantitative RT-PCR expression analysis, recombinant expression in transgenic Nicotiana benthamiana leaves under the driving of CaMV35S promoter, recombinant coexpression with substrate proteins MKK3 and MKK6 in Saccharomyces cerevisiae strain AH109 cells | Brassica napus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Brassica napus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MKK3 | Brassica napus | substrate Brassica napus MAPK kinase 3 | ADP + phosphorylated MKK3 | - |
? | |
ATP + MKK6 | Brassica napus | substrate Brassica napus MAPK kinase 6 | ADP + phosphorylated MKK6 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | A0A0U1XFB1 | gene MAPKKK4.2 | - |
Brassica napus | A0A0U1XFE3 | gene MAPKKK4.1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MKK3 | substrate Brassica napus MAPK kinase 3 | Brassica napus | ADP + phosphorylated MKK3 | - |
? | |
ATP + MKK6 | substrate Brassica napus MAPK kinase 6 | Brassica napus | ADP + phosphorylated MKK6 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BnaMAPKKK4.2 | - |
Brassica napus |
MAPKKK4 | - |
Brassica napus |
mitogen-activated protein kinase kinase kinase 4 | - |
Brassica napus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Brassica napus |
General Information | Comment | Organism |
---|---|---|
metabolism | MAPKK kinase family enzymes play important roles in plant growth, development and immune responses. The two downstream kinases MKK3 and MKK6 act in the BnaMAPKKK4 signaling pathway | Brassica napus |
physiological function | expression of MAPKKK4 elicits reactive oxgen species, ROS, i.e. H2O2 accumulation and cell death. Upregulation of enzyme MAPKKK4 leads to increased expression of ROS accumulation, cell death, and defense response related genes | Brassica napus |