Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Rad9 protein | Saccharomyces cerevisiae | a DNA damage response mediator | ADP + Rad9 phosphoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Rad9 protein | a DNA damage response mediator | Saccharomyces cerevisiae | ADP + Rad9 phosphoprotein | - |
? | |
ATP + Rad9 protein | a DNA damage response mediator, phosphorylaation at Thr125 and Thr143 | Saccharomyces cerevisiae | ADP + Rad9 phosphoprotein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cdc28 | - |
Saccharomyces cerevisiae |
CDK | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
physiological function | a specific G2/M form of Cdc28 can phosphorylate in vitro the N-terminal region of Rad9 on nine consensus CDK phosphorylation sites, N-terminal CDK consensus sites are phosphorylated by Cdc28/Clb2 and are required for activation of Chk1. The integrity of CDK consensus sites and the activity of Cdc28 are required for both the activation of the Chk1 checkpoint kinase and its interaction with Rad9. Phosphorylation of T143 is the most important feature promoting Rad9/Chk1 interaction, while the much more abundant phosphorylation of the neighbouring T125 residue impedes the Rad9/Chk1 interaction. CDK-dependent activation of Chk1 controls Rad9/Chk1 interaction | Saccharomyces cerevisiae |