Literature summary for 2.7.11.1 extracted from

Akizuki, K.; Toyama, T.; Yamashita, M.; Sugiyama, Y.; Ishida, A.; Kameshita, I.; Sueyoshi, N.
Facile preparation of highly active casein kinase 1 using Escherichia coli constitutively expressing lambda phosphatase (2018), Anal. Biochem., 549, 99-106 .

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant CK1 undergoes autoinactivation via autophosphorylation in Escherichia coli cells and thus is undesirably prepared as a phosphorylated and inactivated kinase. To circumvent this problem, a protein expression system is established using Escherichia coli strain BL21(DE3)plambdaPP in which lambda protein phosphatase (lambdaPPase) is constitutively expressed. Using this system, recombinant CK1 isoforms (alpha, delta and epsilon) are readily prepared as unphosphorylated forms. CK1s prepared using BL21(DE3)plambdaPP show markedly higher activity than those prepared by the conventional BL21(DE3). The kinase activity of CK1delta from BL21(DE3)plambdaPP is higher than that prepared by a conventional method consisting of troublesome steps such as in vitro lambdaPPase treatment. The simple method using BL21(DE3)plambdaPP is valuable for preparing highly active CK1s	Mus musculus

Cloned (Comment)

Organism

recombinant CK1 undergoes autoinactivation via autophosphorylation in Escherichia coli cells and thus is undesirably prepared as a phosphorylated and inactivated kinase. To circumvent this problem, a protein expression system is established using Escherichia coli strain BL21(DE3)plambdaPP in which lambda protein phosphatase (lambdaPPase) is constitutively expressed. Using this system, recombinant CK1 isoforms (alpha, delta and epsilon) are readily prepared as unphosphorylated forms. CK1s prepared using BL21(DE3)plambdaPP show markedly higher activity than those prepared by the conventional BL21(DE3). The kinase activity of CK1delta from BL21(DE3)plambdaPP is higher than that prepared by a conventional method consisting of troublesome steps such as in vitro lambdaPPase treatment. The simple method using BL21(DE3)plambdaPP is valuable for preparing highly active CK1s

Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q8BK63	-	-
Mus musculus	Q9DC28	-	-
Mus musculus	Q9JMK2	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Mus musculus	-

Synonyms

Synonyms	Comment	Organism
casein kinase 1	-	Mus musculus
CK1alpha	-	Mus musculus
CK1delta	-	Mus musculus
CK1epsilon	-	Mus musculus

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Release 2023.1 (January 2023)