Cloned (Comment) | Organism |
---|---|
gene lmo1820, encodes PrkA, expression of His6-tagged PrkA catalytic domain in Escherichia coli strain M15 | Listeria monocytogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | a pattern of basic residues is followed by a predicted transmembrane domain suggesting that the N-terminal region (residues 1-338) is orientated toward the cytoplasm | Listeria monocytogenes | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Listeria monocytogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
x * 72000, about, sequence calculation of the full-length enzyme, x * 39000-43000, several forms of the isolated recombinant His6-tagged catalytic domain, SDS-PAGE | Listeria monocytogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Listeria monocytogenes | - |
two eukaryotic-type Ser/Thr-kinases encoded by lmo1820 and lmo0618 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | PrkA is able to autophosphorylate specificly the residues Thr171, Thr174, and Thr176 within the sequence 160-183 of the PrkA activation loop, mass spectrometry analysis of tryptic peptides, overview | Listeria monocytogenes |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged PrkA catalytic domain from Escherichia coli strain M15 by nickel affinity chromatography | Listeria monocytogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myelin basic protein | - |
Listeria monocytogenes | ADP + phosphorylated myelin basic protein | - |
? | |
additional information | PrkA is able to autophosphorylate specific Thr residues within its activation loop sequence | Listeria monocytogenes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, about, sequence calculation of the full-length enzyme, x * 39000-43000, several forms of the isolated recombinant His6-tagged catalytic domain, SDS-PAGE | Listeria monocytogenes |
More | the C-terminal domain sequence of PrkA shows the presence of several copies of PASTA domains, i.e. penicillin-binding protein and Ser/Thr kinase associate | Listeria monocytogenes |
Synonyms | Comment | Organism |
---|---|---|
PrkA | - |
Listeria monocytogenes |
serine/threonine protein kinase | - |
Listeria monocytogenes |
StpK | - |
Listeria monocytogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Listeria monocytogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Listeria monocytogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Listeria monocytogenes |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Listeria monocytogenes | PrkA, sequence calculation | - |
4.99 |
General Information | Comment | Organism |
---|---|---|
additional information | the C-terminal domain sequence of PrkA shows the presence of several copies of extracellular PASTA domains, i.e. penicillin-binding protein and Ser/Thr kinase associate, a wel conserved intracellular kinase domain, and a N-terminal region (residues 1-338), which is orientated toward the cytoplasm | Listeria monocytogenes |
physiological function | PrkA probably is involved in the regulation of a variety of fundamental biological processes, functional classification of the PrkAc putative interactors, overview | Listeria monocytogenes |